Identification of functional apple scab resistance gene promoters

Silfverberg-Dilworth, E.; Besse, S.; Paris, Roberta; Belfanti, E.; Tartarini, Stefano; Sansavini, S.; Patocchi, Andrea; Gessler, C.

doi:10.1007/s00122-005-1940-9

Cited by 20 publications

(17 citation statements)

References 29 publications

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“…3), but this was below the expression obtained with the 288-and 799-bp fragments. Although the result is consistent with those previously obtained by Silfverberg-Dilworth et al (2005) using quantitative GUS assessments, we cannot exclude that the integration site and the number of integrations play a role. Both P2-115::HcrVf2 lines contain two copies possibly resulting in posttranscriptional gene silencing.…”

Section: Discussionsupporting

confidence: 88%

“…Silfverberg-Dilworth et al (2005) used different promoter deletion constructs along with the gus gene in transient expression assays. Construct design was based upon differences observed between promoter sequences of the three transcribed genes of the HcrVf cluster HcrVf1, HcrVf2, and HcrVf4.…”

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confidence: 99%

“…From the HcrVf2 promoter, two constructs were designed with 288 and 779 bp of the sequence. The lengths of 288 and 779 bp were chosen because they cover regions of greatest sequence discrepancy between −229 and −667 (Silfverberg-Dilworth et al 2005). Sequences between −1 and −115 bp of all three genes have 95% identity; therefore, it was assumed that results obtained from the promoter region −1 and −115 from HcrVf1 are true for HcrVf2 as well.…”

mentioning

confidence: 99%

“…In this study, we used the previously identified promoter sequences (Silfverberg-Dilworth et al 2005) along with the HcrVf2 gene. Three gene constructs containing the HcrVf2 gene along with different lengths (115, 288, and 779 bp) of the own promoter region were used in stable transformations of the scab-susceptible apple cvs.…”

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Highly scab-resistant transgenic apple lines achieved by introgression of HcrVf2 controlled by different native promoter lengths

Szankowski

Waidmann

Degenhardt

et al. 2008

Tree Genetics & Genomes

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Apple scab, caused by the ascomycete Venturia inaequalis, is the most damaging fungal disease of commercial apple orchards. Functional scab resistance genes are present in some wild Malus species. The HcrVf2 gene, derived from the Vf-region of the wild apple Malus floribunda 821 and encoding a receptor-like protein, has proved to confer scab resistance in a transgenic susceptible cultivar. In order to minimize nonplant DNA in genetically modified apple and to go a step toward the development of cisgenic apples, we have studied the capability of the HcrVf2 gene to confer apple scab resistance when it is controlled by its own promoter. Three promoter deletion constructs containing 115, 288, and 779 bp of the 5′ untranslated region and the HcrVf2 gene were used to transform the scab susceptible apple cvs. 'Gala' and 'Elstar.' The influence of the promoter length on both the HcrVf2 expression level and the response to V. inaequalis was analyzed in different transgenic lines. Promoter length was found to influence both the constitutive transcription levels of HcrVf2 in transgenic lines and the resistance level. Highly scab resistant 'Elstar' and 'Gala' plants were obtained, proving that the HcrVf2 gene controlled by its native promoter is effective in conferring resistance to V. inaequalis similarly as Vf introgressed in apple cvs. through classical breeding.

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Section: Discussionsupporting

confidence: 88%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Highly scab-resistant transgenic apple lines achieved by introgression of HcrVf2 controlled by different native promoter lengths

Szankowski

Waidmann

Degenhardt

et al. 2008

Tree Genetics & Genomes

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“…At 21 days postinoculation, leaves were prepared for microscopy by following the methods of Silfverberg-Dilworth et al (24).…”

Section: Methodsmentioning

confidence: 99%

The HcrVf2 gene from a wild apple confers scab resistance to a transgenic cultivated variety

Belfanti

Silfverberg-Dilworth

Tartarini

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

230

137

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The Vf gene from the wild species Malus floribunda 821 is the most studied apple scab resistance gene. Several molecular markers mapping around this gene were the starting point for a positional cloning project. The analysis of the bacterial artificial chromosome clones spanning the Vf region led to the identification of a cluster of genes homologous to the Cladosporium fulvum resistance gene family of tomato. One of these genes, HcrVf2 (homologue of the C. fulvum resistance genes of the Vf region), was used to transform the susceptible apple cultivar Gala. Four independent transformed lines resistant to apple scab were produced, proving that HcrVf2 is sufficient to confer scab resistance to a susceptible cultivar. The results show that direct gene transfer between cross-compatible species can be viable when, as in apple, the use of backcrosses to introduce resistance genes from wild species cannot exactly reconstitute the heterozygous genotype of clonally propagated cultivars.

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Apple

Malnoy

Korban

Boresjza-Wysocka

et al. 2008

Compendium of Transgenic Crop Plants

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The first report of transformed apple plants in 1989 raised expectations for new apple cultivars that would be better tasting, healthier, and easier to grow. Although, many different traits have now been introduced successfully into apple, no transformed cultivars have yet made it to commercial production. Most early reports on transformed apple described “proof of concept” experiments involving the development of regeneration protocols, and the choice of appropriate promoters and selectable markers. More recently, the focus has moved onto functional testing of traits of potential commercial interest. These traits can be grouped into two categories: horticultural production traits and fruit‐focused traits. Production traits of interest include bacterial, fungal and pest resistance, dwarfing, propagation, stress resistance, precocity, storage life, and self‐fertility. Examples of fruit‐focused traits include novel health properties, flavor, reduced browning, color, and reduced allergenicity. This review will consider reports of characters introduced into apple that are useful to growers and consumers, and looks toward future trends, targets, and challenges.

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Identification of functional apple scab resistance gene promoters

Cited by 20 publications

References 29 publications

Highly scab-resistant transgenic apple lines achieved by introgression of HcrVf2 controlled by different native promoter lengths

Highly scab-resistant transgenic apple lines achieved by introgression of HcrVf2 controlled by different native promoter lengths

The HcrVf2 gene from a wild apple confers scab resistance to a transgenic cultivated variety

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