Identification of fusarium head blight resistance related metabolites specific to doubled-haploid lines in barley

Chamarthi, Sivakumar K.; Kumar, K.; Gunnaiah, Raghavendra; Kushalappa, Ajjamada C.; Dion, Yves; Choo, T. M.

doi:10.1007/s10658-013-0302-8

Cited by 34 publications

(34 citation statements)

References 46 publications

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“…Anti-fusarium metabolites have also been explored in chickpeas, and their effect was tested for protection of wheat from FHB [27]. Additionally, investigations of metabolites commonly involved in biotic responses [21,36], as well as plant hormones with possible roles in resistance [7,18,37] have also been performed. Although there is some overlap in metabolites that accumulate as a response to biotic stress across these distinct experiments and across different species, there are also many unique metabolites identified in the different reports.…”

Section: Resultsmentioning

confidence: 99%

Metabolomics and Cheminformatics Analysis of Antifungal Function of Plant Metabolites

Čuperlović-Culf¹,

Rajagopalan²,

Tulpan³

et al. 2016

Metabolites

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Fusarium head blight (FHB), primarily caused by Fusarium graminearum, is a devastating disease of wheat. Partial resistance to FHB of several wheat cultivars includes specific metabolic responses to inoculation. Previously published studies have determined major metabolic changes induced by pathogens in resistant and susceptible plants. Functionality of the majority of these metabolites in resistance remains unknown. In this work we have made a compilation of all metabolites determined as selectively accumulated following FHB inoculation in resistant plants. Characteristics, as well as possible functions and targets of these metabolites, are investigated using cheminformatics approaches with focus on the likelihood of these metabolites acting as drug-like molecules against fungal pathogens. Results of computational analyses of binding properties of several representative metabolites to homology models of fungal proteins are presented. Theoretical analysis highlights the possibility for strong inhibitory activity of several metabolites against some major proteins in Fusarium graminearum, such as carbonic anhydrases and cytochrome P450s. Activity of several of these compounds has been experimentally confirmed in fungal growth inhibition assays. Analysis of anti-fungal properties of plant metabolites can lead to the development of more resistant wheat varieties while showing novel application of cheminformatics approaches in the analysis of plant/pathogen interactions.

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Section: Resultsmentioning

confidence: 99%

Metabolomics and Cheminformatics Analysis of Antifungal Function of Plant Metabolites

Čuperlović-Culf¹,

Rajagopalan²,

Tulpan³

et al. 2016

Metabolites

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“…For spore production, F. graminearum was further subcultured on Rye B agar media and kept inverted by exposing the plates to near UV light for a period of 3 days. Macroconidia were harvested from 7-day-old cultures, spore concentration was determined using a haemocytometer (American Scientific, Ohio, USA), and the final concentration was adjusted to 1 9 10 5 macroconidia/mL (Chamarthi et al, 2014). At 50% anthesis (GS = 65), three alternate spikelet pairs of ten spikes per replicate were individually inoculated with 10 lL of fungal spore suspension containing of 1 9 10 5 macroconidia/mL or mock solution (water) using a syringe with an autodispenser (GASTIGHT 1750DAD, Reno).…”

Section: Pathogen Production and Inoculationmentioning

confidence: 99%

Identification and characterization of a fusarium head blight resistance gene TaACT in wheat QTL‐2DL

Kage

Karre

Kushalappa

et al. 2016

Plant Biotechnology Journal

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SummaryFusarium head blight (FHB) resistance in wheat is considered to be polygenic in nature. Cell wall fortification is one of the best resistance mechanisms in wheat against Fusarium graminearum which causes FHB. Metabolomics approach in our study led to the identification of a wide array of resistance‐related (RR) metabolites, among which hydroxycinnamic acid amides (HCAAs), such as coumaroylagmatine and coumaroylputrescine, were the highest fold change RR metabolites in the rachis of a resistant near‐isogenic line (NIL‐R) upon F. graminearum infection. Placement of these metabolites in the secondary metabolic pathway led to the identification of a gene encoding agmatine coumaroyl transferase, herein referred to as TaACT, as a candidate gene. Based on wheat survey sequence, TaACT was located within a FHB quantitative trait loci on chromosome 2DL (FHB QTL‐2DL) between the flanking markers WMC245 and GWM608. Phylogenetic analysis suggested that TaACT shared closest phylogenetic relationship with an ACT ortholog in barley. Sequence analysis of TaACT in resistant and susceptible NILs, with contrasting levels of resistance to FHB, led to the identification of several single nucleotide polymorphisms (SNPs) and two inversions that may be important for gene function. Further, a role for TaACT in FHB resistance was functionally validated by virus‐induced gene silencing (VIGS) in wheat NIL‐R and based on complementation studies in Arabidopsis with act mutant background. The disease severity, fungal biomass and RR metabolite analysis confirmed TaACT as an important gene in wheat FHB QTL‐2DL, conferring resistance to F. graminearum.

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“…Secologanin production is induced by the application of methyl jasmonate in C. roseus, perhaps suggesting a link between defense-related signaling pathways and monoterpene indole alkaloid production. A study using double haploid barley lines differing in Fusarium head blight sensitivity observed metabolite accumulation and found secologanin was constitutively produced in resistant lines [54]. Few alkaloid compounds have been identified within wheat.…”

Section: Alkaloidsmentioning

confidence: 99%

Fusarium Mycotoxins and Metabolites that Modulate Their Production

Jiménez-García¹,

García-Mier²,

García‐Trejo³

et al. 2018

Fusarium - Plant Diseases, Pathogen Diversity, Genetic Diversity, Resistance and Molecular Markers

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The genus Fusarium is a group of fungi producing several types of toxins with toxicological effect in both humans and animals. Such fungi are commonly found in soils so it can contaminate various types of crops, preferably cereals, leading to significant economic losses. Relative humidity, storage temperature and various handling in cereales increase the possibility of contamination by Fusarium toxins. Cereals naturally have secondary metabolites that may help attenuate contamination by these toxins, but it is necessary to know strategies and mechanisms that generate inactivation mycotoxins. This chapter reviews relevant information about cereal mycotoxin contamination, as well as the production of cereal secondary metabolites as a strategy to reduce the possibility of mycotoxin contamination.

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Identification of fusarium head blight resistance related metabolites specific to doubled-haploid lines in barley

Cited by 34 publications

References 46 publications

Metabolomics and Cheminformatics Analysis of Antifungal Function of Plant Metabolites

Metabolomics and Cheminformatics Analysis of Antifungal Function of Plant Metabolites

Identification and characterization of a fusarium head blight resistance gene TaACT in wheat QTL‐2DL

Fusarium Mycotoxins and Metabolites that Modulate Their Production

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