Identification of Fusarium Species Associated with Onion (&amp;lt;i&amp;gt;Allium cepa&amp;lt;/i&amp;gt; L.) Plants in Field in Burkina Faso

Kintega, Konwende Raïssa; Zida, P. Elisabeth; Tarpaga, Vianney W.; Sankara, Philippe; Sereme, P.

doi:10.4236/abb.2020.113008

Cited by 8 publications

(6 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This has been observed in laboratory and greenhouse. Thus, the pathogenicity of the species identified in this study was confirmed, as already mentioned by some authors (Haapalainen et al, 2016;Nasr Esfahani, 2018;Kintega et al, 2020b;Tirado-Ramirez et al, 2021). All the strains used, in this study also, showed an inhibitory effect on coleoptile growth.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Pathogenic Characterization of Three Fusarium Species Associated with Onion (Allium cepa L.) In Burkina Faso

Hamidou¹,

Koïta²,

Alassane³

et al. 2022

Int. J. Phytopathol.

View full text Add to dashboard Cite

Basal rot is a widespread onion disease caused by fungi belonging to Fusarium genus. The disease is known to incur onion yield loss worldwide. This study aims to identify and determine the pathogenicity of Fusarium species associated with basal rot of onions in Burkina Faso. Thirteen Fusarium isolates from seven regions of Burkina Faso were identified based on morphological observations and molecular diagnosis and their pathogenicity was assessed in laboratory and Greenhouse. Results showed that, the 13 isolates belonged to Fusarium falciforme, Fusarium acutatum and Fusarium oxysporum species. F. falciforme, F. acutatum and F. oxysporum were pathogenic on onion seeds, seedlings and bulbs. F. falciforme caused lower seedling damping-off rate compared to that caused by F. acutatum and F. oxysporum. All these species of Fusarium genus induced onion bulbs rots. The results of this study confirmed the presence of these species in Burkina Faso and that they are pathogenic on onion. Therefore, it would be useful to suggest an implement sustainable management approach of these pathogens.

show abstract

Section: Discussionsupporting

confidence: 89%

“…The same quantity of water was used for the controls. Rot length was observed for each treatment after two weeks of incubation and was noted by measurement (Kalman et al, 2020;Kintega et al, 2020b).…”

Section: Fusarium Species Effects On Bulbs Assaymentioning

confidence: 99%

Pathogenic Characterization of Three Fusarium Species Associated with Onion (Allium cepa L.) In Burkina Faso

Hamidou¹,

Koïta²,

Alassane³

et al. 2022

Int. J. Phytopathol.

View full text Add to dashboard Cite

show abstract

“…The pathogenicity of 33 monospore isolates of Fusarium belonging to five species: Fusarium oxysporum (13 isolates); Fusarium proliferatum (15 isolates); Fusarium solani (2 isolates); Fusarium fujikuroi (2 isolates); Fusarium thapsinum (1 isolate), was assessed on onion seedlings. These isolates were collected from onion plants from 17 production sites in Burkina Faso: Yako, Ouahigouya, Titao, Korsimoro, Kongoussi, Mogtédo, Loumbila, Donsin, Kokologho, Koudougou, Réo, Gnassan, Di, Tougan, Dano, Soumasso and Bama (Figure 1) and identified using molecular technology [24].…”

Section: Pathogenicity Assessment Of 33 Isolates Of Different Fusariu...mentioning

confidence: 99%

Determination of <i>Fusarium</i> Species Associated with Onion Plants (<i>Allium cepa</i>) in Field in Burkina Faso Causing Damping-Off and Bulb Rots

Kintega¹,

Zida²,

Soalla³

et al. 2020

AJPS

Self Cite

View full text Add to dashboard Cite

Onion (Allium cepa L.) is the second most important vegetable crop in Burkina Faso and provides an important source of income for those involved in the sector. However, producers are facing significant pre-harvest and post-harvest losses. To date, knowledge of major diseases of the crop is limited, limiting the development of effective control strategies. The objective of this study was to test the pathogenicity of some species of Fusarium. To this end, 33 fungal isolates collected from onion plants in 17 localities and belonging to five Fusarium species were used to inoculate onion seeds and bulbs to determine the pathogenic species responsible for damping-off on seedlings and basal bulb rot in Burkina Faso. The virulence of pathogenic isolates was determined according to the percentages of seedling damping-off evaluated 28 Days After Sowing, and the extent of rot in millimetres on inoculated bulbs. The evaluation of isolates on seedlings revealed that the most pathogenic isolates belong to the species F. proliferatum (I29, I21, I37, I33, I31), F. thapsinum (I35) and F. solani (I38) which resulted in 58.33% -70.83% of seedling damping-off. The most pathogenic isolates on bulbs belong to the species F. proliferatum (I4, I29, I32) and F. oxysporum (I52, I50, I16) which caused 21.67 to 25 mm of rot on bulbs. Isolate I29 was very virulent on both seedlings and bulbs. The isolates of F. fujikuroi species were all low pathogenic on seedlings but one of them, (I27), expressed average pathogenicity on bulbs.

show abstract

“…Previous studies suggested that molecular analysis is generally preferred for characterization and identification of Fusarium species, and provides easy detection of the differences in this species (Nayyar et al, 2018;Guan et al, 2020). Combinations of both morphological observations and molecular characters are necessary to accurately identify Fusarium species (Zhu et al, 2014;Chang et al, 2018;Guan et al, 2020;Kintega et al, 2020) Geiser et al, 2004;Geiser et al, 2005;O'Donnell et al, 2009O'Donnell et al, , 2010 conducted molecular studies relating to the phylogenetic relationship of Fusarium species. Likewise, it was stated that an ITS of the rDNA gene has been widely used for the identification and phylogenetic analysis of Fusarium species by many researchers (Nilsson et al, 2010;Schoch et al, 2012;Jedidi et al, 2018;Minati and Mohammed-Ameen, 2019;Moummi et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Fusarium incarnatum Causing Fusarium Wilt on Protea (Protea cynaroides L.) in Turkey

Çat

2022

Türkiye Tarımsal Araştırmalar Dergisi

View full text Add to dashboard Cite

Protea (Protea cynaroides L.) is one of the most important ornamental plants commercially cultivated in Turkey and other parts of the world. In May 2017, protea plants heavily showed wilting symptoms were obtained from the protea growing areas at Hatay province of Turkey. Brown vascular tissue in the stems of the infected plants was observed. To determine the causal agent of the disease, necrotic tissue pieces taken from diseased plants were surface-sterilized with 10% sodium hypochlorite, two times rinsed with sterile distilled water, placed on Petri dishes containing potato dextrose agar (PDA), and incubated at 24 oC for seven days. Microconidia were no septate, ovoid, hyaline, single-celled, and 9 to 12 × 1 to 3 μm. Macroconidia were slightly curved, four to six septate, and 27 to 31 × 3 to 5 μm. Based on the morphology and cultural features, the fungus was identified as F. incarnatum, which belongs to the F. incarnatum-equiseti species complex (FIESC). In addition, a pathogenicity test was made using the apple fruits. The pathogen was re-isolated from inoculated apple fruits and both cultural and morphological characteristics of the pathogen were identical. To confirm the identity of one representative isolate, the internal transcribed spacer (ITS) region including 5.8S rDNA was amplified and sequenced with primers of ITS-1 and ITS-4. Fusarium MLST, Fusarium-ID and GenBank database were used for the identification of sequence. The amplified 465 bp product has 99% nucleotide identity with the sequences of F. incarnatum-equiseti species complex of MLST types and deposited in GenBank under accession number (MH005097). According to the literature, this is the first molecular identification of F. incarnatum on protea plants in Turkey.

show abstract

Identification of Fusarium Species Associated with Onion (<i>Allium cepa</i> L.) Plants in Field in Burkina Faso

Cited by 8 publications

References 9 publications

Pathogenic Characterization of Three Fusarium Species Associated with Onion (Allium cepa L.) In Burkina Faso

Pathogenic Characterization of Three Fusarium Species Associated with Onion (Allium cepa L.) In Burkina Faso

Determination of <i>Fusarium</i> Species Associated with Onion Plants (<i>Allium cepa</i>) in Field in Burkina Faso Causing Damping-Off and Bulb Rots

Fusarium incarnatum Causing Fusarium Wilt on Protea (Protea cynaroides L.) in Turkey

Contact Info

Product

Resources

About

Identification of Fusarium Species Associated with Onion (&lt;i&gt;Allium cepa&lt;/i&gt; L.) Plants in Field in Burkina Faso

Cited by 8 publications

References 9 publications

Pathogenic Characterization of Three Fusarium Species Associated with Onion (Allium cepa L.) In Burkina Faso

Pathogenic Characterization of Three Fusarium Species Associated with Onion (Allium cepa L.) In Burkina Faso

Determination of &lt;i&gt;Fusarium&lt;/i&gt; Species Associated with Onion Plants (&lt;i&gt;Allium cepa&lt;/i&gt;) in Field in Burkina Faso Causing Damping-Off and Bulb Rots

Fusarium incarnatum Causing Fusarium Wilt on Protea (Protea cynaroides L.) in Turkey

Contact Info

Product

Resources

About

Identification of Fusarium Species Associated with Onion (<i>Allium cepa</i> L.) Plants in Field in Burkina Faso

Determination of <i>Fusarium</i> Species Associated with Onion Plants (<i>Allium cepa</i>) in Field in Burkina Faso Causing Damping-Off and Bulb Rots