Identification of Fusarium verticillioides Resistance Alleles in Three Maize Populations With Teosinte Gene Introgression

Abstract

Fusarium ear rot (FER) is a common fungal disease in maize (Zea mays L.) caused by Fusarium verticillioides. Resistant germplasm resources for FER are rare in cultivated maize; however, teosintes (Z. mays ssp. parviglumis and Z. mays ssp. diploperennis), which are wild-type species of maize, have the potential to offer a novel source of resistance alleles to enhance pathogen resistance in modern maize. Therefore, the aim of this study was to identify favorable alleles that confer significant levels of resistance toward FER. Three populations of BC2F8 recombinant inbred lines (RILs) were developed by crossing two different teosintes, Z. diploperennis and Z. parviglumis, with maize inbred lines B73 and Zheng58, and were screened for FER resistance. We found that Z. diploperennis and Z. parviglumis had higher resistance toward F. verticillioides in the leaves than B73 and Zheng58. However, the resistance toward F. verticillioides in the leaf and ear was unrelated among RILs. FER resistance was positively correlated with grain yield in the B73 × diploperennis (BD) and Zheng58 × parviglumis (ZP) populations, partly because the quantitative trait loci (QTLs) of FER resistance and yield traits were located close together. Four coincident QTLs (qFERbd5.177, qFERbd10.140, qFERzp4.066, and qFERzp5.116) and two highly reliable resistance-yield synergistic QTLs (qFERbd10.140 and qFERzp4.066) were identified in the BD and ZP populations, opening up the possibility of breeding for FER resistance without reducing yield.

Mapping of QTL for resistance to fusarium stalk rot (FSR) in tropical maize (Zea mays L.)

“…In DHF 2 s of the cross VL121096 × CM202 (MP2), the QTL detected showed near major effects in both the populations (qFSR_7_2, qFSR_6_2, qFSR_7_3, and qFSR_7_4). Several others also detected major effect QTL for resistance to Fusarium ear rot on chromosomes 6 and 7 as in present study (Perez-Brito et al 2001;Robertson-Hoyt et al 2006;Ding et al 2008;Chen et al 2012;Maschiettoet al 2017;Giomiet al 2021;Rashid et al 2022;Feng et al 2022). Resistant parent CM212 contributed the favourable alleles for QTLs detected in the cross VL1043 × CM212.…”

“…Quantitative trait loci were mapped for FSR on chromosomes 1, 5 and 10 in MP1 but all with minor effects. Similarly, Giomi et al (2021), Rashid et al 2021 andFeng et al (2022) also reported QTLs on these chromosomes. Liu et al (2021) reported QTL in bins 7.02, 7.04, 10.03, and 10.04, which explained variations with a range from 1.39% to 2.04%.…”

“…While, in VL121096 × CM202 favourable alleles for QTLs were contributed by resistant parent CM202 except for qFSR_7_3 detected in winter, 2019-20 and q_FSR_7_4 detected in combined QTL analysis. The QTL detected on a linkage group 10 (qFSR_10_1) was stable across two populations which could be used successfully in transferring resistance to FSR and annotation of this genomic region might result in the identification of useful genes for resistance (Feng et al 2022).…”

Meta-analysis and co-expression analysis revealed stable QTL and candidate genes conferring resistances to Fusarium and Gibberella ear rots while reducing mycotoxin contamination in maize

“…Finally, 22 publications satisfied our inclusion criteria and were therefore considered for full text screening. Fifteen publications were SNP-based QTL mapping studies which were used to collect relevant information required for the QTL meta-analysis ( Supplementary File 2 ) ( Chen et al., 2016 ; Giomi et al., 2016 ; Han et al., 2016 ; Kebede et al., 2016 ; Maschietto et al., 2017 ; Han et al., 2018 ; Galić et al., 2019 ; Wen et al., 2020 ; Yuan et al., 2020a ; Galiano-Carneiro et al., 2021 ; Giomi et al., 2021 ; Wen et al., 2021b ; Zhou et al., 2021 ; Feng et al., 2022 ; Guo et al., 2022 ). Seven papers were related to genome-wide association study and used to cross-validate the meta-analysis ( Butrón et al., 2019 ; Samayoa et al., 2019 ; Wu et al., 2020 ; Gaikpa et al., 2021 ; Gesteiro et al., 2021 ; Liu et al., 2021 ; da Silva et al., 2022 ).…”

The effect of using fresh farmyard manure (animal manure) on the severity of Fusarium verticilioides in soil, root, stem, and kernels as well as lodging and borer incidence of maize plants

“…For instance, Fusarium stalk reduces maize yield by 10% and 30–50% in severely affected areas ( Li et al., 2010 ). Similarly, ( Feng et al, 2022 ) demonstrated that Fusarium species attack the stem base of maize plants, causing stalk rot – usually during August and September. A relationship has been found between the aetiology of the Fusarium that produces stalk rot and ear rot.…”