Identification of G-Proteins Coupling to the Vasoactive Intestinal Peptide Receptor VPAC1 Using Immunoaffinity Chromatography: Evidence for Precoupling

Shreeve, S. Martin

doi:10.1006/bbrc.2002.6342

Cited by 15 publications

(9 citation statements)

References 45 publications

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“…Any of these actions would cause a VGCC‐dependent inhibition of GABA release. The fact that the VPAC 1 receptor is operating in a G i ‐dependent (instead of a G s ‐dependent) manner is not surprising as the ability of the VPAC 1 receptor to couple to G i proteins has previously been observed in CHO cells and hippocampal membranes (van Rampelbergh et al ., ; Shreeve, ).…”

Section: Discussionmentioning

confidence: 99%

“…However, VPAC 1 and VPAC 2 receptors can also couple to other signalling/G protein-dependent mechanisms in different brain preparations (Fatatis et al, 1994;Gressens et al, 1998;Nielsen et al, 2002). Furthermore, VPAC 1 receptor couples to G i/o proteins in the hippocampus (Shreeve, 2002), and VPAC 1 -mediated enhancement of synaptic transmission in the CA1 area of the hippocampus is dependent on both PKA and PKC activity (Cunha-Reis et al, 2005). This suggests that VIP receptors can couple to different signalling pathways in the hippocampus.…”

Section: Introductionmentioning

confidence: 99%

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VPAC₁ and VPAC₂ receptor activation on GABA release from hippocampal nerve terminals involve several different signalling pathways

Cunha‐Reis

Ribeiro

Almeida

et al. 2017

British J Pharmacology

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Our results show that VPAC and VPAC VIP receptors have opposing actions on GABA release from hippocampal nerve terminals through activation of different transduction pathways. As VPAC and VPAC receptors are located in different layers of Ammon's horn, our results suggest that these VIP receptors underlie different modulation of synaptic transmission to pyramidal cell dendrites and cell bodies, with important consequences for their possible therapeutic application in the treatment of epilepsy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

VPAC₁ and VPAC₂ receptor activation on GABA release from hippocampal nerve terminals involve several different signalling pathways

Cunha‐Reis

Ribeiro

Almeida

et al. 2017

British J Pharmacology

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“…Our data were obtained using two complementary approaches that have been used already for either identifying the coupling between a receptor and a G protein or estimating the efficacy of this coupling (Shreeve, 2002). The immunoaffinity copurification technique relies on the high specificity of the antiserum immobilized on the immunoaffinity column.…”

Section: Discussionmentioning

confidence: 99%

Regional Differences in the Coupling of 5-Hydroxytryptamine-1A Receptors to G Proteins in the Rat Brain

Cour

Mestikawy²,

Hanoun³

et al. 2006

Mol Pharmacol

125

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Numerous data showed that 5-hydroxytryptamine-1A (5-HT 1A ) receptors couple to G␣ o /␣ i proteins for signal transduction. However, the ␣ subunit isoforms really involved in 5-HT 1A receptor coupling in brain remain to be identified. Moreover, regional differences in the functional characteristics of brain 5-HT 1A receptors have been evidenced repeatedly. Because such differences could be due to variations in G proteins interacting with the same receptor, relevant approaches were used for identifying ␣ subunits physically coupled to 5-HT 1A receptors in different regions of the rat brain. Using immunoaffinity chromatography coupled to Western blot detection, 5-HT 1A receptors were found to interact equally with G␣ o and G␣ i3 in the cerebral cortex, mainly with G␣ o and weakly with G␣ i3 in the hippocampus and exclusively with G␣ i3 in the anterior raphe area. In the hypothalamus, 5-HT 1A receptors seemed to be coupled to the latter two G proteins plus G␣ i1 and G␣ z . Complementary experiments based on an antibody capture technique coupled to both classic radioactivity and scintillation proximity assay detections showed that hippocampal 5-HT 1A receptor stimulation induced 5Ј-O-(3-[35 S]thio)triphosphate binding to immunoprecipitates with G␣ i3 and G␣ o antisera. In the anterior raphe, such 5-HT 1A receptor-mediated effect was obtained with G␣ i3 antiserum only. These results demonstrated the existence of regional differences in the coupling of 5-HT 1A receptors to G proteins in the rat brain. In the anterior raphe, 5-HT 1A receptors seem to interact specifically with G␣ i3 , whereas in the hippocampus, they are mainly coupled to G␣ o proteins. Such a disparity in G-protein coupling might explain regional differences in adaptive regulations of brain 5-HT 1A receptors.Because of its involvement in various psychiatric pathologies, such as affective disorders, the 5-HT 1A type of serotonin (5-hydroxytryptamine, 5-HT) receptors is a subject of great interest. 5-HT 1A receptor stimulation has been shown to activate different signaling pathways sensitive to pertussis toxin through G␣ i /G␣ o proteins (Raymond et al., 1993). Inhibition of adenylyl cyclase (AC) activity mediated by 5-HT 1A receptors has been evidenced in most brain regions as a result of G␣ i protein activation (De Vivo and Maayani, 1986;Hamon, 1997). In addition, in the hippocampus, 5-HT 1A receptors have been shown to mediate G-protein-gated inwardly rectifying K ϩ current (GIRK) through a direct interaction of the ionic channel with the ␤␥ subunits of the G␣ o isoform (Andrade and Nicoll, 1987;Oleskevich, 1995).The development of heterologous recombinant systems expressing the 5-HT 1A receptor, such as COS-7, HeLa, Chinese hamster ovary, Sf9, GH4C1, LLC-PK1, and NIH-3T3 transfected cells, has provided relevant models to study the receptor coupling with different G-protein subtypes and to identify

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“…In fact, such a basis exists, because 5-HT 7 receptors (as opposed to the 5-HT 4(b) receptor) exhibit multiple properties similar to other G protein-coupled receptors known to form a tight complex with G protein in the absence of ligand (Roka et al, 1999;Vasquez and Lewis, 1999;Mukhopadhyay et al, 2000;Shreeve, 2002). For example, a very high fraction of 5-HT 7 receptors exist in a high-affinity agonist binding state, which is insensitive to the destabilizing effect of guanine nucleotides (Alberts et al, 2001;Krobert et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…Experimental support for the existence of receptors tightly associated (preassociated) to their respective G protein, in the absence of ligand, has been reported for the CB 1 -cannabinoid receptor (Vasquez and Lewis, 1999;Mukhopadhyay et al, 2000), the Mel 1a melatonin receptor (Roka et al, 1999) and the vasoactive intestinal peptide VPAC 1 receptor (Shreeve, 2002). Although the CB 1 receptor-G␣ i/o association is sensitive to the destabilizing effect of guanine nucleotides (Mukhopadhyay et al, 2000), high-affinity agonist binding at the Mel 1a receptor is resistant to both the destabilizing effect of guanine nucleotides and pertussis toxin (Roka et al, 1999).…”

mentioning

confidence: 93%

Activation of Adenylyl Cyclase by Endogenous G_s-Coupled Receptors in Human Embryonic Kidney 293 Cells Is Attenuated by 5-HT₇Receptor Expression

Andressen¹,

Norum²,

Levy³

et al. 2005

Mol Pharmacol

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Human 5-hydroxytryptamine 7 (5-HT 7 ) receptors display characteristics shared with receptors believed to form a tight physical coupling with G protein in the absence of ligand. Some receptors apparently preassociated with G i/o and G q/11 are reported to inhibit the signaling of other similarly coupled G protein-coupled receptors by limiting their access to activate a common G protein pool. Therefore, we determined whether 5-HT 7 receptor expression was sufficient to limit signaling of endogenously expressed G s -coupled receptors in human embryonic kidney (HEK) 293 cells. Using the ecdysone-inducible expression system, which allows for the titration of increasing receptor density in the same clonal cell line, we compared the effects of 5-HT 4(b) and 5-HT 7(a,b,d) receptor expression on adenylyl cyclase (AC) stimulation by the endogenous G s -coupled ␤-adrenergic (␤AR) and prostanoid EP (EPR) receptors. ␤AR-and EPR-stimulated AC activity was attenuated by 5-HT 7 receptor expression in both membrane preparations and intact HEK293 cells. ␤AR-and EPR-stimulated AC activity was unaffected by expression of the G s -coupled 5-HT 4 receptor. The mechanism of this heterologous desensitization seems independent of protein kinase A activation, nor does it occur at the level of G protein activation because 1) ␤AR-and EPR-stimulated AC activity was not restored to control values when G␣ s was overexpressed; and 2) ␤ 1 AR and ␤ 2 AR activation of G␣ s was unaffected by the expression of 5-HT 7 receptors. In addition, overexpression of AC isoforms was unable to rescue ␤AR-and EPR-stimulated AC activity. Therefore, 5-HT 7 receptors probably limit access and/or impede activation of AC by ␤AR and EP receptors. Although the 5-HT 7 receptor may preassociate with G protein and/or AC, the mechanism of this heterologous desensitization remains elusive.Serotonin (5-hydroxytryptamine, 5-HT) mediates its diverse physiological effects through at least 14 different receptor subtypes, of which 13 belong to the G protein-coupled receptor family (Hoyer et al., 1994). Among the human 5-HT receptors, three different subtypes, 5-HT 4 , 5-HT 6 , and 5-HT 7 , are coupled to G s and at least the 5-HT 4 and 5-HT 7 receptors are expressed as several different functional splice variants (Gerald et al., 1995;Heidmann et al., 1997). The functional significance of 5-HT 7 splice variants, which differ only in the carboxyl terminus (Heidmann et al., 1997), remains unknown (Krobert et al., 2001Krobert and Levy, 2002), whereas among the 5-HT 4 splice variants, constitutive activation of AC is dependent on the different carboxyl termini (Bockaert et al., 2004). We have shown previously that the 5-HT 4(b) and 5-HT 7(a) signaling properties differ fundamentally. The potency of 5-HT to stimulate AC increased with increasing receptor density in clones expressing 5-HT 4(b) but not 5-HT 7(a) receptors, even though 5-HT-stimulated AC activity in clones expressing 5-HT 7(a) receptors had reached asymptotic levels (Bruheim et al., 2003). This indicates that...

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Identification of G-Proteins Coupling to the Vasoactive Intestinal Peptide Receptor VPAC1 Using Immunoaffinity Chromatography: Evidence for Precoupling

Cited by 15 publications

References 45 publications

VPAC₁ and VPAC₂ receptor activation on GABA release from hippocampal nerve terminals involve several different signalling pathways

VPAC₁ and VPAC₂ receptor activation on GABA release from hippocampal nerve terminals involve several different signalling pathways

Regional Differences in the Coupling of 5-Hydroxytryptamine-1A Receptors to G Proteins in the Rat Brain

Activation of Adenylyl Cyclase by Endogenous G_s-Coupled Receptors in Human Embryonic Kidney 293 Cells Is Attenuated by 5-HT₇Receptor Expression

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Identification of G-Proteins Coupling to the Vasoactive Intestinal Peptide Receptor VPAC1 Using Immunoaffinity Chromatography: Evidence for Precoupling

Cited by 15 publications

References 45 publications

VPAC1 and VPAC2 receptor activation on GABA release from hippocampal nerve terminals involve several different signalling pathways

VPAC1 and VPAC2 receptor activation on GABA release from hippocampal nerve terminals involve several different signalling pathways

Regional Differences in the Coupling of 5-Hydroxytryptamine-1A Receptors to G Proteins in the Rat Brain

Activation of Adenylyl Cyclase by Endogenous Gs-Coupled Receptors in Human Embryonic Kidney 293 Cells Is Attenuated by 5-HT7Receptor Expression

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VPAC₁ and VPAC₂ receptor activation on GABA release from hippocampal nerve terminals involve several different signalling pathways

VPAC₁ and VPAC₂ receptor activation on GABA release from hippocampal nerve terminals involve several different signalling pathways

Activation of Adenylyl Cyclase by Endogenous G_s-Coupled Receptors in Human Embryonic Kidney 293 Cells Is Attenuated by 5-HT₇Receptor Expression