2006
DOI: 10.1271/bbb.70.646
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Identification of Genes Affecting Lipid Content Using Transposon Mutagenesis inSaccharomyces cerevisiae

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Cited by 43 publications
(33 citation statements)
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“…However, total lipid content in S. pombe cell is not high and around 5% to 10% of dry cell weight (DCW). This value is similar to the level in S. cerevisiae (Kamisaka et al 2006). For instance, the total FA content/DCW of sample C in Fig.…”
Section: Discussionsupporting
confidence: 84%
“…However, total lipid content in S. pombe cell is not high and around 5% to 10% of dry cell weight (DCW). This value is similar to the level in S. cerevisiae (Kamisaka et al 2006). For instance, the total FA content/DCW of sample C in Fig.…”
Section: Discussionsupporting
confidence: 84%
“…3% of fatty acid content, comparable to the values obtained in the systems of Beaudoin et al (2) and Domergue et al (8). The total lipid content of S. cerevisiae is not high (22) and may have to be increased for our long-term goal of producing PUFAs by use of transgenic S. cerevisiae in sustainable quantities.…”
Section: (0)supporting
confidence: 78%
“…NSD was examined because the carbon/nitrogen ratio affects the amount of lipids in the cell (22,32). Prior to this experiment, we preliminarily examined the effect of carbon sources that result in oxidative metabolism, as PUFA production requires an active cytochrome system.…”
Section: Resultsmentioning
confidence: 99%
“…TAG metabolism is well characterized in S. cerevisiae (Sorger and Daum 2003;Czabany et al 2007), and overexpression of DGAT genes has been reported to increase the lipid content in plants and yeasts (Hobbs et al 1999;Bouvier-Nave et al 2000;Jako et al 2001). Kamisaka et al (2006) found that the disruption of SNF2, a gene encoding a transcription factor forming part of the SWI/SNF chromatin-remodeling complex, increased the lipid content in S. cerevisiae. Overexpression of DGA1 (diacylglycerol acyltransferase) and LEU2 genes in the snf2 disruptant has achieved 29 % of total lipid content to DCW, high enough to use this strain as a model of oleaginous yeast (Kamisaka et al 2007).…”
Section: Future Perspectivesmentioning
confidence: 99%