Identification of genes differentially expressed by calorie restriction in the rotifer (Brachionus plicatilis)

Oo, Aung Kyaw Swar; Kaneko, Gen; Hirayama, Makoto; Kinoshita, Shigeharu; Watabe, Shugo

doi:10.1007/s00360-009-0389-6

Cited by 23 publications

(8 citation statements)

References 82 publications

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“…All isolates belong to the B. plicatili s cryptic species complex, made up of at least 13 morphologically similar but genetically distinct groups (Gómez et al, 2002; Mills et al, 2007) that have been the focus of several recent aging studies (Gribble and Mark Welch, 2013; Kaneko et al, 2010; Oo et al, 2010; Snell et al, 2012). Ten of the 12 isolates used in this study were from a single clade (Clade A) within the species complex and are able to interbreed, suggesting any differences in CR have evolved recently (Gribble and Mark Welch, 2012; Snell, 1989; Snell and Hawkinson, 1983; Suatoni et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Patterns of intraspecific variability in the response to caloric restriction

Gribble

Kaido

Jarvis

et al. 2014

Experimental Gerontology

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Caloric restriction (CR) is cited as the most robust means of increasing lifespan across a range of taxa, yet there is a high degree of variability in the response to CR, both within and between species. To examine the intraspecific evolutionary conservation of lifespan extension by CR, we tested the effects of chronic caloric restriction (CCR) at multiple food levels and of intermittent fasting (IF) in twelve isolates from the Brachionus plicatilis species complex of monogonont rotifers. While CCR generally increased or did not change lifespan and total fecundity, IF caused increased, unchanged, or decreased lifespan, depending upon the isolate, and decreased total fecundity in all but one isolate. Lifespan under ad libitum (AL) feeding varied among isolates and predicted the lifespan response to CR: longer-lived isolates under AL were less likely to have a significant increase in lifespan under CCR and were more likely to have a significantly shortened lifespan under IF. Lifespan under AL conditions and the response to CR were not correlated with hydroperiodicity of native habitat or with time in culture. Lack of trade-off between lifespan and fecundity under CCR, and differences in lifespan and fecundity under CCR and IF, even when average food intake was similar, suggest that longevity changes are not always directly determined by energy intake and that CCR and IF regimens extend lifespan through diverse genetic mechanisms.

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Section: Introductionmentioning

confidence: 99%

Patterns of intraspecific variability in the response to caloric restriction

Gribble

Kaido

Jarvis

et al. 2014

Experimental Gerontology

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“…The rotifer Brachionus plicatilis is a zooplankton species used as a model organism in CR studies [12][13][14]. CR induces resistance to oxidative stress evoked by paraquat (1,1-dimethyl-4,4-bipyrimidyl chloride), an exogenous ROS generator [15], whereas the relative mRNA levels of Mn SOD and catalase are enhanced in the rotifer under normal physiological conditions without oxidative stress [16].…”

Section: Introductionmentioning

confidence: 99%

Effects of calorie restriction on the expression of manganese superoxide dismutase and catalase under oxidative stress conditions in the rotifer Brachionus plicatilis

Kailasam

Kaneko

et al. 2011

Fish Sci

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Calorie restriction (CR) in the rotifer Brachionus plicatilis extends its lifespan, as it enhances the expression of antioxidant enzymes such as manganese superoxide dismutase (Mn SOD) and catalase. Here we show that CR also increased the mRNA levels of these antioxidant enzymes upon exposure to oxidative stress. Rotifers cultured under CR showed a higher survival rate than those fed ad libitum (AL) upon exposure to 0.05-0.2 lM juglone, an oxidative stress inducer. The relative mRNA levels of Mn SOD and catalase before exposure to juglone were slightly higher in the CR rotifers than in their AL counterparts, although these differences were not statistically significant. AL rotifers showed no apparent upregulation of the mRNA levels of these antioxidant enzymes upon exposure to 0.025 and 0.05 lM juglone. In contrast, the CR rotifers increased the mRNA levels of Mn SOD and catalase by up to 5.4-fold and 4.2-fold, respectively, resulting in significant differences between their levels in AL and CR rotifers under oxidative stress conditions. Furthermore, the protein level of catalase was clearly higher in CR than in AL rotifers 6 h after exposure to oxidative stress. These results suggest that the upregulation of Mn SOD and catalase genes is involved in CR-induced resistance to oxidative stress in the rotifer.

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“…Genes differentially expressed genes in dietary restricted B. plicatilis were identified by Oo et al (2010). Controls were fed the alga Nannochloropsis oculata continuously whereas the DR treatment was fed only 3 h per day.…”

Section: Dietary Restriction In Rotifersmentioning

confidence: 99%

Rotifers as models for the biology of aging

Snell

2014

Internat. Rev. Hydrobiol.

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It has been two decades since 1993 when research on the biology of rotifer aging was last reviewed by Enesco. Much has transpired during this time as rotifer biologists have adapted to the “omics” revolution and incorporated these techniques into the experimental analysis of rotifers. Rotifers are amenable to many of these approaches and getting adequate quantities of DNA, RNA, and protein from rotifers is not difficult. Analysis of rotifer genomes, transcriptomes, and proteomes is rapidly yielding candidate genes that likely regulate a variety of features of rotifer biology. Parallel developments in aging biology have recognized the limitations of standard animal models like worms and flies and that comparative aging research has essentially ignored a large fraction of animal phylogeny in the lophotrochozoans. As experimentally tractable members of this group, rotifers have attracted interest as models of aging. In this paper, I review advances over the past 20 years in the biology of aging in rotifers, with emphasis on the unique contributions of rotifer models for understanding aging. The majority of experimental work has manipulated rotifer diet and followed changes in survival and reproductive dynamics like mean lifespan, maximum lifespan, reproductive lifespan, and mortality rate doubling time. The main dietary manipulation has been some form of caloric restriction, withholding food for some period or feeding continuously at low levels. There have been comparative studies of several rotifer species, with some species responding to caloric restriction with life extension, but others not, at least under the tested food regimens. Other aspects of diet are less explored, like nutritional properties of different algae species and their capacity to extend rotifer lifespan. Several descriptive studies have reported many genes involved in rotifer aging by comparing gene expression in young and old individuals. Classes of genes up or down-regulated during aging have become prime targets for rotifer aging investigations. Alterations of gene expression by exposure to specific inhibitors or RNAi knockdown will probably yield valuable insights into the cellular mechanisms of rotifer life extension. I highlight major experimental contributions in each of these areas and indicate opportunities where I believe additional investigation is likely to be profitable.

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Identification of genes differentially expressed by calorie restriction in the rotifer (Brachionus plicatilis)

Cited by 23 publications

References 82 publications

Patterns of intraspecific variability in the response to caloric restriction

Patterns of intraspecific variability in the response to caloric restriction

Effects of calorie restriction on the expression of manganese superoxide dismutase and catalase under oxidative stress conditions in the rotifer Brachionus plicatilis

Rotifers as models for the biology of aging

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