Identification of genes essential for anaerobic growth of Listeria monocytogenes

Müller-Herbst, Stefanie; Wüstner, Stefanie; Mühlig, Anna; Eder, D.; Fuchs, Thilo M.; Held, Claudia; Ehrenreich, Armin; Scherer, Siegfried

doi:10.1099/mic.0.075242-0

Cited by 46 publications

(53 citation statements)

References 55 publications

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“…A positive family history of migraine (relatives of first or second grade) was found in 65.2%. A positive family history for periodic syndromes and/or migraine had been reported previously ( 42 , 43 , 51 , 52 ). Parents of children with vertigo/dizziness should always be asked about this.…”

Section: Discussionmentioning

confidence: 62%

Vestibular Migraine in Children and Adolescents: Clinical Findings and Laboratory Tests

et al. 2015

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Introduction: Vestibular migraine (VM) is the most common cause of episodic vertigo in children. We summarize the clinical findings and laboratory test results in a cohort of children and adolescents with VM. We discuss the limitations of current classification criteria for dizzy children.Methods: A retrospective chart analysis was performed on 118 children with migraine related vertigo at a tertiary care center. Patients were grouped in the following categories: (1) definite vestibular migraine (dVM); (2) probable vestibular migraine (pVM); (3) suspected vestibular migraine (sVM); (4) benign paroxysmal vertigo (BPV); and (5) migraine with/without aura (oM) plus vertigo/dizziness according to the International Classification of Headache Disorders, 3rd edition (beta version).Results: The mean age of all patients was 12 ± 3 years (range 3–18 years, 70 females). 36 patients (30%) fulfilled criteria for dVM, 33 (28%) for pVM, 34 (29%) for sVM, 7 (6%) for BPV, and 8 (7%) for oM. Somatoform vertigo (SV) co-occurred in 27% of patients. Episodic syndromes were reported in 8%; the family history of migraine was positive in 65%. Mild central ocular motor signs were found in 24% (most frequently horizontal saccadic pursuit). Laboratory tests showed that about 20% had pathological function of the horizontal vestibulo-ocular reflex, and almost 50% had abnormal postural sway patterns.Conclusion: Patients with definite, probable, and suspected VM do not differ in the frequency of ocular motor, vestibular, or postural abnormalities. VM is the best explanation for their symptoms. It is essential to establish diagnostic criteria in clinical studies. In clinical practice, however, the most reasonable diagnosis should be made in order to begin treatment. Such a procedure also minimizes the fear of the parents and children, reduces the need to interrupt leisure time and school activities, and prevents the development of SV.

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Section: Discussionmentioning

confidence: 62%

Vestibular Migraine in Children and Adolescents: Clinical Findings and Laboratory Tests

et al. 2015

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“…The construction of the in‐frame deletion mutant L. monocytogenes Δ glnR and the in cis complementation mutant L. monocytogenes Δ glnR‐ comp was performed as described previously (Müller‐Herbst et al ., ). Oligonucleotides and plasmids used in this study are listed in Tables S3 and S4.…”

Section: Methodsmentioning

confidence: 97%

“…For RNA isolation, cells were grown in a chemically defined minimal medium until an optical density at 600 nm (OD 600 nm ) of 0.80–0.87 and harvested [4200 g , 8 min, room temperature (RT)], and the pellets were shock frozen in liquid nitrogen and stored at −80 °C until further processing. RNA isolation was performed as described (Müller‐Herbst et al ., ).…”

Section: Methodsmentioning

confidence: 97%

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Temperature- and nitrogen source-dependent regulation of GlnR target genes inListeria monocytogenes

Kaspar

Auer

Schardt

et al. 2014

FEMS Microbiol Lett

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The ubiquitous pathogen Listeria monocytogenes lives either saprophytically in the environment or within cells in a vertebrate host, thus adapting its lifestyle to its ecological niche. Growth experiments at 24 and 37 °C (environmental and host temperature) with ammonium or glutamine as nitrogen sources revealed that ammonium is the preferred nitrogen source of L. monocytogenes. Reduced growth on glutamine is more obvious at 24 °C. Global transcriptional microarray analyses showed that the most striking difference in temperature-dependent transcription was observed for central nitrogen metabolism genes, glnR (glutamine synthetase repressor GlnR), glnA (glutamine synthetase GlnA), amtB (ammonium transporter AmtB), glnK (PII regulatory protein GlnK), and gdh (glutamate dehydrogenase) when cells were grown on glutamine. When grown on ammonium, both at 24 and 37 °C, the transcriptional level of these genes resembles that of cells grown with glutamine at 37 °C. Electrophoretic mobility shift assay studies and qPCR analyses in the wild-type L. monocytogenes and the deletion mutant L. monocytogenes ∆glnR revealed that the transcriptional regulator GlnR is directly involved in temperature- and nitrogen source-dependent regulation of the respective genes. Glutamine, a metabolite known to influence GlnR activity, seems unlikely to be the (sole) intracellular signal mediating this temperature-and nitrogen source-dependent metabolic adaptation.

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“…Before assembly, adaptor sequences, empty reads and low-quality sequences were removed. Data evaluation was performed according to the description of Müller-Herbst et al [63]. To annotate isotigs and singletons, the non-redundant sequences were searched against the protein databases obtained from NCBI with a search threshold of E -value cut-off 10 −5 [18].…”

Section: Methodsmentioning

confidence: 99%

Comparative Transcriptional Analysis of Loquat Fruit Identifies Major Signal Networks Involved in Fruit Development and Ripening Process

Song

Zhao

et al. 2016

IJMS

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Loquat (Eriobotrya japonica Lindl.) is an important non-climacteric fruit and rich in essential nutrients such as minerals and carotenoids. During fruit development and ripening, thousands of the differentially expressed genes (DEGs) from various metabolic pathways cause a series of physiological and biochemical changes. To better understand the underlying mechanism of fruit development, the Solexa/Illumina RNA-seq high-throughput sequencing was used to evaluate the global changes of gene transcription levels. More than 51,610,234 high quality reads from ten runs of fruit development were sequenced and assembled into 48,838 unigenes. Among 3256 DEGs, 2304 unigenes could be annotated to the Gene Ontology database. These DEGs were distributed into 119 pathways described in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A large number of DEGs were involved in carbohydrate metabolism, hormone signaling, and cell-wall degradation. The real-time reverse transcription (qRT)-PCR analyses revealed that several genes related to cell expansion, auxin signaling and ethylene response were differentially expressed during fruit development. Other members of transcription factor families were also identified. There were 952 DEGs considered as novel genes with no annotation in any databases. These unigenes will serve as an invaluable genetic resource for loquat molecular breeding and postharvest storage.

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Identification of genes essential for anaerobic growth of Listeria monocytogenes

Cited by 46 publications

References 55 publications

Vestibular Migraine in Children and Adolescents: Clinical Findings and Laboratory Tests

Vestibular Migraine in Children and Adolescents: Clinical Findings and Laboratory Tests

Temperature- and nitrogen source-dependent regulation of GlnR target genes inListeria monocytogenes

Comparative Transcriptional Analysis of Loquat Fruit Identifies Major Signal Networks Involved in Fruit Development and Ripening Process

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