Identification of genes expressed after noise exposure in the chick basilar papilla

Gong, Tzy-Wen; Hegeman, Adrian D.; Shin, Jouyoung J.; Adler, Henry J.; Raphael, Yehoash; Lomax, Margaret I.

doi:10.1016/0378-5955(96)00013-5

Cited by 53 publications

(34 citation statements)

References 49 publications

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“…AF188735) was cloned in a differential display (Liang and Pardee, 1992;Liang et al, 1993) experiment designed to identify genes that are differentially expressed in the chick cochlea after noise overstimulation (acoustic trauma) and during regeneration of the auditory epithelium (Gong et al, 1996). This cDNA sequence had high identity to the 3Ј-UTRs of mouse, Xenopus, and ze- brafish ZIC2 cDNAs, suggesting that we had cloned the chick ZIC2 cDNA.…”

Section: Isolation Of Chick Probes For Zic Genesmentioning

confidence: 99%

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Expression of ZIC genes in the development of the chick inner ear and nervous system

Warner

Hutson

et al. 2003

Developmental Dynamics

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ZIC genes, vertebrate homologues of the Drosophila pair-rule gene odd-paired (opa), function in embryonic pattern formation, in the early stages of central nervous system neurogenesis and in cerebellar maturation. Mouse Zic genes are expressed in restricted, and in some cases overlapping, patterns during development, particularly in the central and peripheral nervous systems. We identified chick ZIC2 in a differential display analysis of the auditory system designed to find genes up-regulated after noise trauma. In this study, we examined the expression of chick ZIC1, ZIC2, and ZIC3 by in situ hybridization in normal inner ear development and in the tissues that influence its development, including the hindbrain, the neural crest, and the periotic mesenchyme. Between Hamburger and Hamilton stages 13 and 24, all three ZIC genes were found in the dorsal periotic mesenchyme adjacent to the developing inner ear. ZIC1 mRNA was expressed in the otocyst epithelium between stages 12 and 24, in some sensory tissue, as well as in a striped pattern in the floorplate of the hindbrain that appears to be complementary to that of Chordin, a gene known to regulate ZIC expression in frogs. Chick ZIC genes are also expressed in the neuroectoderm, paraxial mesenchyme, brain, spinal cord, neural crest, and/or the overlying ectoderm as well as the limb buds. In general, ZIC1 and ZIC2 expression patterns overlapped, although ZIC2 expression was less robust; ZIC3 expression was minimal. These observations suggest that ZIC genes, in addition to their known roles in brain development, may play an important role in the development of the chick inner ear. Developmental Dynamics 226:702-712, 2003.

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Section: Isolation Of Chick Probes For Zic Genesmentioning

confidence: 99%

“…In earlier studies, we identified a partial chick cDNA for ZIC2 in a differential display experiment designed to find genes whose expression is elevated after noise trauma in the chicken (Gong et al, 1996). Chick inner ear sensory epithelium, unlike that of mammals, is capable to some extent of regeneration (Stone and Rubel, 2000).…”

Section: Introductionmentioning

confidence: 99%

Expression of ZIC genes in the development of the chick inner ear and nervous system

Warner

Hutson

et al. 2003

Developmental Dynamics

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“…This over stimulation induces a softening of the hair bundle that spontaneously resolves in a manner thought to result from a restructuring of the cytoskeleton within the stereocilia (Duncan et al 1995;Duncan and Saunders 2000). Noise trauma increases expression of cdc-42 in the sensory epithelium of the chick cochlea (Gong et al 1996). This finding is significant because cdc-42 is involved in regulating actin dynamics (Hall 1998).…”

Section: Introductionmentioning

confidence: 99%

The Involvement of Arl-5b in the Repair of Hair Cells in Sea Anemones

Watson

Graugnard

Mire

2007

JARO

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The subcellular processes involved in repair of hair cells are not well understood. Sea anemones repair hair bundle mechanoreceptors on their tentacles after severe trauma caused by 1-h exposure to calcium-depleted seawater. Repair is dependent on the synthesis and secretion of large protein complexes named Brepair proteins.^A cDNA library on traumatized anemone tissue was probed using polyclonal antibodies raised to a specific chromatographic fraction of the repair protein mixture. An ADPribosylation factor-like protein, Arl-5b, was identified. The amino acid sequence of the Arl-5b protein in sea anemones is similar to that among several model vertebrates and humans. A polyclonal antibody raised to a peptide of the anemone Arl-5b labels some but not all hair bundles in healthy control animals. The abundance of labeled hair bundles significantly increases above healthy controls after trauma and continuing through the first hour of recovery. Dilute anti-Arl-5b blocks the spontaneous repair of hair bundle mechanoreceptors, suggesting that Arl-5b acts on the extracellular face of the plasma membrane. Immunoelectron microscopy indicates that Arl-5b is located along the length of stereocilia including sites in the vicinity of tip links. We propose that Arl-5b is involved in installing replacement linkages into damaged hair bundle mechanoreceptors.

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“…mRNA from the basilar papilla of normal chicks was compared with that obtained from chicks exposed to an octave band noise (center frequency 1.5 kHz, 118 dB, 6 h) and from chicks exposed to noise and allowed to recover for 2 days. Among the chick cDNAs identified were the parathyroid hormone-related protein, an immediate early gene; the delta-subunit of the neuronal-specific Ca 2+ /calmodulin-regulated protein kinase II, the GTP-binding protein CDC42 and a novel chick WDR1 gene [Adler et al, 1999;Gong et al, 1996]. WDR1 encodes a protein that mediates actin interaction with other proteins.…”

Section: Avian Hair Cells Regeneratementioning

confidence: 99%

From Gene Identification to Gene Therapy

Kanzaki¹,

Kawamoto²,

Oh³

et al. 2002

Audiol Neurotol

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Inner ear disease due to hair cell loss is common, and no restorative treatments for the balance and hearing impairment are currently available. To develop clinical means for enhancing protection and regeneration in the inner ear, it is necessary to understand the molecular basis for hereditary and acquired deafness and vestibular disorders. One approach is to identify and characterize genes that regulate protection or repair in other systems. For that purpose, we have used the differential display assay and compared gene expression between normal and acoustically traumatized inner ears of chicks. Several chick cDNAs that were identified are considered as candidates for roles in the reparative process that follows trauma in the basilar papilla. The mammalian vestibular epithelium has a limited regenerative capability. To identify genes that may participate in the regenerative response, we have used gene arrays profiling, comparing normal to drug-traumatized vestibular epithelia. We identified several genes that are differentially expressed in traumatized vestibular epithelium, including several insulin-like growth factor-I binding proteins. To use this molecular knowledge for enhancing protection and repair in the organ of Corti, it is necessary to overexpress the genes of choice in the inner ear. Using viral-mediated gene transfer, we overexpressed transgenic glial cell line-derived neurotrophic factor and demonstrated a robust protective effect against acoustic and ototoxic inner ear trauma. Future identification of the genes that are important for protection and regeneration, along with improved gene transfer technology, will allow the use of gene therapy for treating hereditary and environmental inner ear disease.

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Identification of genes expressed after noise exposure in the chick basilar papilla

Cited by 53 publications

References 49 publications

Expression of ZIC genes in the development of the chick inner ear and nervous system

Expression of ZIC genes in the development of the chick inner ear and nervous system

The Involvement of Arl-5b in the Repair of Hair Cells in Sea Anemones

From Gene Identification to Gene Therapy

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