Identification of genes expressed in the liver of Japanese flounder Paralichthys olivaceus by expressed sequence tags

Abstract

A list of genes expressed in the liver of Japanese flounder was compiled using an expressed sequence tag (EST) strategy; 940 EST derived from liver cDNA clones were determined. Bioinformatic analysis revealed that 537 (57%) of the EST could be ascribed to the transcriptional products of identified genes including 397 previously described in fish. The identified genes were classified with respect to a cellular role, which showed that 314 (58%) of the identified genes encoded proteins associated with primary liver functions such as transport, acute phase response, and blood clotting. Furthermore, comparative analysis revealed that 26 unique genes had homologous to known genes highly expressed in mammalian liver systems. cDNA clones corresponding to antimicrobial peptide origin in the mammalian liver, the hepcidin-like peptide, had conserved cysteine residues similar to the previously identified hepcidin-like peptide type II from winter flounder and were also found in Japanese flounder.

Expression Analysis of Selected Immune‐Relevant Genes in Channel Catfish during Edwardsiella ictaluri Infection

“…See Figure 3 for additional details. Wright et al 1996;Hirono and Aoki 1997;Gabay and Kushner 1999;Stafford and Belosevic 2003;Arma et al 2005). The EB1, b1-integrin, and Hsp70 genes displayed differences in their expression levels among the tissues tested.…”

Heparin cofactor II (RbHCII) from rock bream (Oplegnathus fasciatus): Molecular characterization, cloning and expression analysis

“…In addition, the recently established Fugu rubripes and Tetradon nigroviridis fish genome databases contain most hemostatic and serpin genes. Although such genes have been identified in different fish species, including serpins such as ATIII from Atlantic halibut [39], A1AT, ATIII, C1 inhibitor from Japanese flounder [40], only a few of them have been characterized, for instance, factor VII, IX, X, prothrombin, ATIII and HCII from zebrafish [41], a SPI from carp [42] and ATIII from salmon [43]. In this study, we report a novel heparin cofactor II gene from rock bream, O. fasciatus.…”

Identification of a novel C-type lectin gene in Japanese flounder, Paralichthys olivaceus

“…A total of 2087 Japanese flounder liver EST sequences in DDBJ/EMBL/GenBank database including those reported so far [18,19] were assembled using the CAP3 program [20]. The most abundant contigs containing EST sequences were selected and homology searches were performed using the BLASTX program [21].…”