Identification of genes regulated by oleic acid in Jurkat cells by suppressive subtractive hybridization analysis

Abstract

In this study, the effect of oleic acid (50 microM) on gene expression of Jurkat cells (human T lymphocytes cell line) was examined using the suppressive subtractive hybridization approach. This technique allowed us to identify genes with higher or lower expression after cell treatment with oleic acid as compared to untreated cells. Oleic acid upregulated the expression of the translation elongation factor alpha 1 and ATP synthase 8 and downregulated gp96 (human tumor rejection antigen gp96), heat-shock protein 60 and subtilisin-like protein 4. These results suggest that oleic acid, at plasma physiological concentration, can regulate the expression of important genes to maintain the machinery that ensures cell functioning.

An Ethanolic Extract of Cucurbita pepo L. Seeds Modifies Neuroendocrine Disruption in Chronic Stressed Rats and Adrenal Expression of Inflammatory Markers and HSP70

“…This might point to the beneficial effect of the relatively high level of HSP70 induced by both FLU and CP as it resulted in the reduction of caspase-3–mediated apoptosis and release of inflammatory cytokines. Although the effect of pumpkins on heat shock protein family was not previously described, oleic acid, which represents the main constituents of pumpkins used in this study, was described to downregulate the expression of the HSP60 in the human T lymphocyte cell line (Martins De Lima et al, 2004 ), which is supportive to our study finding. The proposed mechanism of action of Cucurbita pepo as an antidepressant-like substance is summarized in Figure 7 .…”

Mechanisms by which fatty acids regulate leucocyte function

“…Our group has also used the suppressive subtractive hybridization approach to identify genes with high or low expression after treatment of Jurkat cells with OA (50 µmol/l) for 24 h. OA was shown to regulate the expression of important genes, such as the translation elongation factor α1, ATP synthase 8, gp96 (human tumour rejection antigen gp96), Hsp60 and SPC4 (subtilisin-like protein 4) [47]. These results suggest that OA, at physiological plasma concentrations, can regulate the expression of important genes in maintaining the machinery that ensures normal cell function.…”

Avian influenza virus infection induces differential expression of genes in chicken kidney

“…Briefly, the DNA probes specific for the GST, G6PC, SytXV, MGC4504, ADH, MGC68696, ISG12, LY6E, and GAPDH gene were separately generated by PCR and labeled with DIG as described above. Total RNA (20 lg) from control and HPAIV-infected chicken kidney tissues were fractionated by electrophoresis on agarose-formaldehyde gels, and then transferred onto nylon membranes as previously described (Martins de Lima et al, 2004). The bound RNA was hybridized with individual DIG-probe at 42°C for 16 h.…”