Identification of genes related to mechanical stress in human periodontal ligament cells using microarray analysis

Araujo, R M S de; Oba, Yasuo; Moriyama, Keiji

doi:10.1111/j.1600-0765.2006.00906.x

Cited by 63 publications

(43 citation statements)

References 56 publications

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“…IL-6 is a strong osteoclast activator (22) that increase in PDL http://bmbreports.org cells in response to static compressive force (5,6). IL-6 decreased in expression after 12 hours of static tensional force ( Table 2).…”

Section: Genes Related To New Bone Formationmentioning

confidence: 99%

“…Recently, the molecular mechanism of bony resorption in the compressive side was investigated by DNA microarray technology and a threedimensional cell culture system (5,6). There it was shown the expression of interleukin-6 (IL-6), a strong osteoclastogenesisactivating cytokine, increased in response to a static compressive force in PDL cells (5,6). Moreover, the activity of alkaline phosphatase (ALP) in PDL cells decreased by the same static compressive force (5).…”

Section: Introductionmentioning

confidence: 99%

“…These responses to mechanical stress have been used for orthodontic tooth movement and both are dependent on the intensity and duration of the applied force (4). Recently, the molecular mechanism of bony resorption in the compressive side was investigated by DNA microarray technology and a threedimensional cell culture system (5,6). There it was shown the expression of interleukin-6 (IL-6), a strong osteoclastogenesisactivating cytokine, increased in response to a static compressive force in PDL cells (5,6).…”

Section: Introductionmentioning

confidence: 99%

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Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture

Ku¹,

Chang²,

Chae³

et al. 2009

BMB Reports

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Orthodontic tooth movement results from the combinational process of both bone resorption and formation in the compressive and tension sides, respectively. However, the genes responsible for new bone formation in tension sides have not been determined. In this study, we used DNA microarray and real-time RT-PCR to identify genes in human periodontal ligament (PDL) cells that undergo significant changes in expression in response to static tensional forces (2 or 12 hours). The genes found were alkaline phospatase (ALP), matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and several collagen genes. Furthermore, an ELISA evaluating the expression of VEGF, type IV collagen and MMP-2 found levels significantly increased after 24 and 72 hours (P ＜ 0.05). ALP activity was also increased after 24 hours (P ＜ 0.05). Collectively, we found the genes up-regulated in our study by the static tensional force are related to osteogenic processes such as matrix synthesis and angiogenesis. [BMB reports 2009; 42(7): 427-432]

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Section: Genes Related To New Bone Formationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture

Ku¹,

Chang²,

Chae³

et al. 2009

BMB Reports

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“…According to the literature, this distinction extends to inflammatory mediators as well. Static compression can upregulate IL-6 (40), PGE2, and COX2 (14), supporting the catabolic side of remodeling, whereas tension can suppress IL-1b-induced upregulation of PGE2, COX2, and MMP-1 and -3 (43).…”

Section: Discussionmentioning

confidence: 90%

Pneumatic pressure bioreactor for cyclic hydrostatic stress application: mechanobiology effects on periodontal ligament cells

Wenger

El‐Awady²,

Messer³

et al. 2011

Journal of Applied Physiology

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“…Interestingly, de Araujo et al (33) reported a microarray analysis of human PDL cells under compressive forces using an in vitro three-dimensional culture system. They identified several up-regulated genes, including inflammation-related molecules, such as COX-2 (cyclooxygenase-2), PGE 2 (prostaglandin E 2 ), IL-6 (interleukin-6), and IL-1␤ (interleukin-1␤), which were not listed in our present study.…”

Section: Discussionmentioning

confidence: 99%

Role of Mechanical Stress-induced Glutamate Signaling-associated Molecules in Cytodifferentiation of Periodontal Ligament Cells

Fujihara

Yamada

Ozaki

et al. 2010

Journal of Biological Chemistry

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The ability of cells to sense and respond to physical stress is required for tissue homeostasis and normal development. In muscle, bone, tendon, periodontium, and the cardiovascular system, applied forces of physiological magnitude regulate cellular processes that are critical for normal tissue and organ functions, such as differentiation, proliferation, and migration (1). The periodontal ligament (PDL) 3 is a connective tissue interposed between the roots of teeth and the inner wall of the tooth-supporting bone (alveolar bone) socket. The PDL constitutively and iatrogenically receives mechanical stress, such as occlusal pressure and orthodontic forces, which have effects on the homeostasis of the PDL (2). Proper mechanical stress on teeth induces not only the proliferation and differentiation of PDL cells into osteoblasts and cementoblasts but also the synthesis and degradation of extracellular matrix (ECM) molecules (3). For example, during orthodontic tooth movement, two types of sites (tension sites and pressure sites) arise around the tooth through the orthodontic force. At the tension sites, the PDL is stretched, and the expressions of bone-related genes, such as osteocalcin (4) and bone sialoprotein (5), are up-regulated, such that bone formation is finally induced on the alveolar bone facing the tooth root (6). On the other hand, at the pressure sites, the PDL is compressed, and osteoclasts are activated. Consequently, resorption of the alveolar bone is induced. An orchestrated balance between bone formation and resorption controls tooth movement (7). In contrast, elimination of mechanical stress on teeth is known to cause atrophy of the PDL in vivo (8). Kaneko et al. (9) reported that loss of occlusal function by extraction of the antagonistic upper molars of rats caused atrophic changes in the PDL of the lower molars, such as narrowing of the space, disorientation of collagen fibers, and decreases in proteoglycans. These findings indicate that mechanical stress on teeth affects the remodeling of the PDL, cementum, and alveolar bone. Thus, it is important to clarify the physiological functions of mechanical stress on the PDL.To clarify the molecular basis of the mechanical stress-regulated PDL functions, we analyzed the gene expression profile of human PDL cells receiving tensile mechanical stress in vitro. Interestingly, an oligo-DNA chip analysis identified two glutamate signaling-associated genes, HOMER1 (homer homolog 1) and GRIN3A (glutamate receptor ionotropic N-methyl-D-aspartate 3A), among the up-regulated genes. L-Glutamate is the most abundant amino acid in the central nervous system and plays important roles in neurotransmission (10

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Identification of genes related to mechanical stress in human periodontal ligament cells using microarray analysis

Abstract: Our microarray is the first to show the gene profile in PDL cells caused by mechanical stress; however, further studies to clarify the physiological function of these molecules in PDL cells are required.

Cited by 63 publications

References 56 publications

Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture

Static tensional forces increase osteogenic gene expression in three-dimensional periodontal ligament cell culture

Pneumatic pressure bioreactor for cyclic hydrostatic stress application: mechanobiology effects on periodontal ligament cells

Role of Mechanical Stress-induced Glutamate Signaling-associated Molecules in Cytodifferentiation of Periodontal Ligament Cells

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