Identification of Genes Specific for Viability of Prostate Cancer Cells using a Pooled Lentiviral shRNA Expression Library

Tedesco, Donato; Makhanov, Mikhail; Bonneau, Kyle; Diehl, Paul; Deng, Dan; Chenchik, Alex

doi:10.2144/000113830

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Screening Strategies To Uncover Synthetic Interactions Using1

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2014

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“…We also have run a tissue-specific dropout viability study in-house to identify genes specifically lethal to androgen-receptor positive prostate cancer cells using a small shRNA library targeting 1,200 prostate-cancer associated human genes. This small screen identified cyclin D1 as specifically essential in these cells but not in androgen receptor negative variants (Figure 2) [33]. …”

Section: Screening Strategies To Uncover Synthetic Interactions Usingmentioning

confidence: 99%

Use of RNAi screens to uncover resistance mechanisms in cancer cells and identify synthetic lethal interactions

Diehl

Tedesco

Chenchik

2014

Drug Discovery Today: Technologies

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RNAi loss-of-function screens, which have proven effective to identify genes functionally responsible for cellular phenotypes, can be designed to use different genetic backgrounds or altered environmental conditions to elucidate genetic dependencies. These sorts of screening approaches can be exploited to identify genetic targets that minimize resistance to approved drugs, and provide a basis on which to develop new targeted therapies and predict the secondary targets for combinatorial treatments. Four types of pooled short hairpin RNA (shRNA) screens, in particular, have been used to look for genetic targets that work together with known drugs or other anticancer targets, either in an additive or synergistic fashion. Each method produces results that provide a useful but limited picture of the genetic elements driving oncogenesis.

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Section: Screening Strategies To Uncover Synthetic Interactions Usingmentioning

confidence: 99%

Use of RNAi screens to uncover resistance mechanisms in cancer cells and identify synthetic lethal interactions

Diehl

Tedesco

Chenchik

2014

Drug Discovery Today: Technologies

View full text Add to dashboard Cite

show abstract

Identification of Genes Specific for Viability of Prostate Cancer Cells using a Pooled Lentiviral shRNA Expression Library

Cited by 1 publication

References 0 publications

Use of RNAi screens to uncover resistance mechanisms in cancer cells and identify synthetic lethal interactions

Use of RNAi screens to uncover resistance mechanisms in cancer cells and identify synthetic lethal interactions

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