Identification of genetic diversity among mutant lemon and mandarin varieties using different molecular markers

Sülü, Görkem; Kaçar, Yıldız Aka; Polat, İlknur; Kitapçı, Aytül; Turgutoğlu, Ertuğrul; Şimşek, Özhan; Satar, Gül

doi:10.3906/tar-1909-67

Cited by 18 publications

(6 citation statements)

References 46 publications

(72 reference statements)

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“…ISSR marker clustering analysis is associated with differences in phenotypic traits ( Wu et al., 2020 ), and this identification technique can help distinguish between morphologically distinct plants; it can also provide a solution for early seedling targeted breeding to screen for mutants, as the undirected nature of mutagenesis leads breeders to overlook invisible resistance indicators. Sulu et al. (2020) also used this approach to demonstrate induced mutations and genetic differentiation of citrus and lemon mutant genotypes as an effective tool for determining plant genotypes.…”

Section: Discussionmentioning

confidence: 99%

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

Lai

et al. 2023

Front. Plant Sci.

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Plumbago indica L. is a perennial herb with ornamental and anticancer medicinal functions widely distributed in the tropics. It is affected by temperature and cannot bloom normally in colder subtropical regions, which seriously affects its ornamental value. To create low-temperature resistance mutants and enrich new germplasm resources, this study used tissue culture and chemical reagent (0.5 mmol/L NaN3) and low-temperature stress (0°C, full darkness for 48h) induction to target and screen for cold-resistance mutants. The results showed that the ISSR band polymorphism ratio of the 24 suspected mutant materials was 87.5%. The DNA profiles of the 9 mutants initially identified were altered. The content of plumbagin in the stems and leaves of the mutants was examined, and it was found that the accumulation in the leaves of the mutant SA24 could be as high as 3.84 times that of the control, which was 0.5991%. There were significant differences in the anatomical structures of roots, stems and leaves. The mutants mostly exhibited reduced root diameter (only 0.17-0.69 times that of CK), increased stem diameter (up to 2.19 times that of CK), enlarged mesophyll cells, increased thickness (up to 1.83 times that of CK) and high specificity, which are thought to be important for the different cold resistance obtained by the mutants. In the cold resistance experiment, four cold-tolerant mutants were successfully screened according to their morphological characteristics and physiological indexes, and the mutagenesis efficiency could be as high as 2.22% and did not affect the accumulation of plumbagin in their stems and leaves, even higher than CK. The responses of the screened mutants SA15, SA19, SA23 and SA24 to low temperature showed slower leaf wilting, higher light energy conversion efficiency, less accumulation of MDA content, increased enzymatic activities of antioxidant enzymes (SOD, CAT, POD) and more accumulation of soluble sugars and proline content. These characteristics are consistent with the response of cold-resistance plants to low temperatures. The cold- resistance mutants cultivated in soil were observed of agronomic and ornamental traits for one year, mainly manifested as delayed flowering and delayed entry into the senescence stage. This study provides a more rapid and accurate technique for identifying and screening cold-tolerant mutants, and lays the foundation for future experiments on the creation of new cold-resistant varieties.

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Section: Discussionmentioning

confidence: 99%

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

Lai

et al. 2023

Front. Plant Sci.

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“…The higher value of I reflects the effectiveness of SSR markers used to identify the variation created by irradiation treatment. Several reports of utilisation of molecular markers for the detection of genetic diversity in mutant population are available in different fruit crops like citrus (Sülü et al, 2020), grapes (Dev et al, 2021), olive (Tekin et al, 2022), and pomegranate (Perveen et al, 2023b). SSR markers have been widely preferred for germplasm characterisation and phylogenetic studies in mango (Azam et al, 2018;Azam et al, 2019;Arogundade et al, 2022).…”

Section: Characterisation Of Putative Mutant Progenies Using Ssr Markersmentioning

confidence: 99%

Volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango (Mangifera indica L.)

Perveen,

Dinesh,

Sankaran

et al. 2023

Front. Plant Sci.

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IntroductionPutative mutants were generated through gamma irradiation in the polyembryonic mango genotype Nekkare. The putative mutant progenies along with control seedlings and mother plants were evaluated by comparing the compositions and relative proportions of their major volatile compounds.MethodsVolatile profiling was done using headspace-solid phase micro-extraction (HS SPME) method coupled with gas chromatography–mass spectrometry (GC-MS MS). Furthermore, characterisation of putative mutants and control seedlings was carried out using simple sequence repeat (SSR) markers to ascertain the genetic diversity present in the samples under study.ResultsMonoterpenes were the most abundant volatile compound in all the studied samples (ranging from 34.76% to 91.41%) out of which I-Phellandrene and cis-Ocimene formed the major fraction in mother plants (20.45%–21.86% and 16.17%–21.27%, respectively) and control seedlings (23.32%–24.95% and 18.95%–20.81%, respectively), while beta-Phellandrene was dominant in the selected putative mutant samples (2.34%–29.53%). Among sesquiterpenes, trans-Caryophyllene was detected only in the putative mutant samples (0.10%–30.18%). Grouping together of mother plants and control seedlings was seen in the cluster analysis, while the putative mutants grouped apart from them suggesting genetic diversity. Genetic distance between the mother plants and control seedlings ranged from 0.97 to 2.73, while between putative mutants, control seedlings, and mother plants, it ranged from 6.54 to 9.82. SSR-based characterisation of putative mutant seedlings showed that mutation caused variability in the treated population. This was evident from the high allelic richness ranging from 4 to 12 with a mean of 7 and a higher mean Shannon’s Information Index (1.50) of the putative mutant population.DiscussionThe study demonstrates that volatile profiling and molecular characterisation using SSR markers could be used as a tool to detect variation in a mutated population. In addition, volatile profiling can be used to validate putative mutants in polyembryonic mango genotypes where the seedlings of nucellar origin are similar to mother plants.

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“…Thusly, in previous studies, differences in cultivars were put forth with principal component analysis in walnuts [42,[59][60][61], kiwifruit [62], strawberry tree [63], hazelnuts [33], almonds [35], raspberry [64], citrus [65] and apricot [66]. All above studies indicated that PCA allows the extraction of the maximum information from used cultivars and underlining the interrelations between variables and individuals, either by similarity or opposition.…”

Section: Eigen Statistics For Two Principal Componentsmentioning

confidence: 99%

Morphological Characteristics of Grapevine Cultivars and Closed Contour Analysis with Elliptic Fourier Descriptors

Küpe

Sayinci

Demır

et al. 2021

Plants

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Morphology is the most visible and distinct character of plant organs and is accepted as one of the most important tools for plant biologists, plant breeders and growers. A number of methods based on plant morphology are applied to discriminate in particular close cultivars. In this study, image processing analysis was used on 20 grape cultivars (“Amasya beyazı“, “Antep karası“, “Bahçeli karası”, “Çavuş“, “Cevşen“, “Crimson“, “Dimrit“, “Erenköy beyazı“, “Hafızali“, “Karaşabi“, “Kırmızı“, “İzabella (Isabella) “, “Morşabi“, “Müşgüle“, “Nuniya“, “Royal“, “Sultani çekirdeksiz (Sultanina)“, “Yalova incisi“, “Yerli beyazv“, “Yuvarlak çekirdeksiz“) to classify them. According to image processing analysis, the longest and the greatest projected area values were observed in “Antep karası“ cultivar. The “Sultani çekirdeksiz“ cultivar had the least geometric mean diameter. The greatest sphericity ratios were observed in “Yerli beyaz“, “Erenköy beyazı“ and “Amasya beyazı“ cultivars. According to principal component analysis, dimensional attributes were identified as the most significant source of variation discriminant grape cultivars from each other. Morphological differences between the cultivars were explained by sphericity and elongation variables. According to elliptic Fourier analysis (EFA) results, grape morphology largely looks like ellipse and sphere. However, there are some cultivars that look similar to a water drop. The cultivars with similar morphology were identified by a pair-wise comparison test conducted with the use of linear discriminant analysis, and they were presented in a scatter plot. According to cluster analysis, present grape cultivars were classified into seven sub-groups, which indicated great diversity.

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Identification of genetic diversity among mutant lemon and mandarin varieties using different molecular markers

Cited by 18 publications

References 46 publications

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

Volatile profiling as a potential biochemical marker for validation of gamma irradiation derived putative mutants in polyembryonic genotypes of mango (Mangifera indica L.)

Morphological Characteristics of Grapevine Cultivars and Closed Contour Analysis with Elliptic Fourier Descriptors

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