Identification of genetic loci affecting the establishment and development of Echinococcus multilocularis larvae in mice

Kameda, Yayoi; Kouguchi, Hirokazu; Matsumoto, Jun; Dang, Zhisheng; Simon, Ayo Yila; Torigoe, Daisuke; Sasaki, Nobuya; Oku, Yuzaburo; Sugimoto, Chihiro; Agui, Takashi; Yagi, Kinpei

doi:10.1016/j.ijpara.2011.06.007

Cited by 11 publications

(10 citation statements)

References 42 publications

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“…1). This region covered highly significant region of the QTL peak detected in the previous QTL analysis (Nakao et al, 2011). 215…”

Section: Confirmation Of Empsc1 Using Congenic Micementioning

confidence: 76%

“…The infection experiments were performed according to Nakao et al, (2011). Briefly, E. multilocularis eggs were collected from feces of the infected dogs.…”

Section: Infection Experiments and Phenotype Assessmentmentioning

confidence: 99%

“…The genetic factors responsible for difference in the susceptibility to E. multilocularis parasite still remains unknown. Considering the marked difference between B6 and D2 mice in susceptibility to E. multilocularis infection, quantitative trait locus (QTL) analysis was conducted in backcrossed progenies from B6 and D2 mice (Nakao et al, 2011). QTL mapping is a promising tool for the detection of genetic loci that contribute to the determination of differences in phenotypic variation.…”

Section: Introductionmentioning

confidence: 99%

“…QTL mapping is a promising tool for the detection of genetic loci that contribute to the determination of differences in phenotypic variation. QTL analysis has revealed a significant QTL, Emcys1 for the establishment of E. multilocularis cysts in the mouse liver, suggesting that it controls the number of cysts in the liver (Nakao et al, 2011). Furthermore, the development of protoscoleces in cysts in the mouse liver were controlled by a distinct highly significant QTL, Empsc1, indicating a role of different host factor interplaying with parasites at each developmental stage (Nakao et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…QTL analysis has revealed a significant QTL, Emcys1 for the establishment of E. multilocularis cysts in the mouse liver, suggesting that it controls the number of cysts in the liver (Nakao et al, 2011). Furthermore, the development of protoscoleces in cysts in the mouse liver were controlled by a distinct highly significant QTL, Empsc1, indicating a role of different host factor interplaying with parasites at each developmental stage (Nakao et al, 2011). Lack of information in the interplay between parasite and the intermediate host makes it difficult to focus on certain genes responsible for resistance/susceptibility to E. multilocularis infection.…”

Section: Introductionmentioning

confidence: 99%

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Analysis for genetic loci controlling protoscolex development in the Echinococcus multilocularis infection using congenic mice

Islam

Torigoe

Kameda

et al. 2018

Infection, Genetics and Evolution

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The resistance/susceptibility to Echinococcus multilocularis infection in mice is genetically controlled. However, genetic factors responsible for these differences remain unknown. Our previous study in genetic linkage analysis has revealed that there is a significant quantitative trait locus (QTL) for the establishment of cyst (Emcys1), and a highly significant QTL for the development of protoscolex of E. multilocularis larvae (Empsc1), on mouse chromosomes 6 and 1, respectively. The current study aimed to confirm these QTLs and narrow down the critical genetic region that controls resistance/susceptibility to E. multilocularis infection by establishing congenic and subcongenic lines from C57BL/6 (B6) and DBA/2 (D2) mice. For protoscolex development phenotype, two congenic lines, B6.D2-Empsc1 and D2.B6-Empsc1 were developed, where responsible QTL, Empsc1 was introgressed from D2 into B6 background and vice versa. For cyst establishment phenotype, two congenic lines, B6.D2-Emcys1 and D2.B6-Emcys1 were developed, where responsible QTL, Emcys1 was introgressed from D2 into B6 background and vice versa. Because there was no significant difference in cyst establishment between B6.D2-Emcys1 and D2.B6-Emcys1 mice after challenge with E. multilocularis, it is suggested that the Emcys1 does not solely control the cyst establishment in mouse liver. However, infection experiments with B6.D2-Empsc1 and D2.B6-Empsc1 mice showed a significant difference in protoscolex development in the cyst. It confirms that the Empsc1 controls phenotype of the protoscolex development in the cyst. Subsequently, two subcongenic lines, B6.D2-Empsc1.1 and B6.D2-Empsc1.2 from B6.D2-Emcys1 and one subcongenic line, D2.B6-Empsc1.1 from D2.B6-Empsc1 were developed to narrow down the critical region responsible for protoscolex development. From the results of infection experiments with E. multilocularis in these subcongenic mice, it is concluded that a gene responsible for protoscolex development is located between D1Mit290 (68.1 cM) and D1Mit511 (97.3 cM).

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“…1). This region covered highly significant region of the QTL peak detected in the previous QTL analysis (Nakao et al, 2011). 215…”

Section: Confirmation Of Empsc1 Using Congenic Micementioning

confidence: 76%

“…The infection experiments were performed according to Nakao et al, (2011). Briefly, E. multilocularis eggs were collected from feces of the infected dogs.…”

Section: Infection Experiments and Phenotype Assessmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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