Identification of Genetic Loci of Black Point in Chinese Common Wheat by Genome-Wide Association Study and Linkage Mapping

Abstract

<jats:p> Black point is a common disease in wheat all over the world. The disease could downgrade wheat quality and cause human health problems. In this study, 406 wheat cultivars were used to investigate black point resistance. In the field tests, 20, 65.5, and 14.5% of the tested cultivars were resistant, moderately resistant, and susceptible, respectively, suggesting that improving black point resistance is necessary in Chinese wheat breeding. A genome-wide association study (GWAS) identified 386 single-nucleotide polymorphisms (SNPs) significantly related to black point resistance in the tested wheat cultivars, and they were located on all chromosomes. Linkage mapping in a biparental population identified three quantitative trait loci (QTL) for black point resistance—QBP.hau-3A, QBP.hau-6D, and QBP.hau-7D—with 6.76, 7.79, and 8.84% phenotypic variation explained, respectively. Based on both the GWAS and linkage analyses, QBP.hau-6D covered six significant SNPs from the GWAS, and the position of these SNPs indicated that this QTL is a new locus for black point resistance. This study provides valuable germplasm for breeding wheat cultivars with resistance to black point and information for further understanding of molecular and genetic basis of black point resistance. </jats:p>

QTL for resistance to black point caused by Bipolaris sorokiniana in bread wheat

“…However, this assumption needs to be veri ed in wheat breeding programs. QBB.hau-5A has been reported in numerous studies (Liu et al 2016, Yang et al 2019, Lv et al 2020Wang et al 2021;Li et al 2020bLi et al , 2022. QBB.hau-5D.1 with a large and consistent effect is likely to be a new locus identi ed in the present study.…”

N6-methyladenosine RNA modification promotes viral genomic RNA stability and infection

“…A total of 362 wheat accessions, composed of an association panel containing 243 current varieties 60 , 61 and 119 historical varieties 62 , 63 , were planted in wheat yellow mosaic virus (WYMV) nursery field in Xiping (N33.40°, E113.3°) in 2016-2017 cropping seasons with one replicate and in the 2017-2018 and 2018-2019 cropping seasons with two replicates. An F 8 RIL (recombinant inbred lines) population composed of 138 lines, derived from the cross between Xianyangdasui (resistant to WYMV) and Xinmai 208 (susceptible to WYMV), was also planted in the same field with two replicates in 2017-2018, 2018-2019, 2019-2020 and 2020-2021 cropping seasons.…”

Genome-Wide Association Study and QTL Mapping Revealed Genetic Loci for Fusarium Head Blight Resistance in Chinese Hexaploid Wheat

“…GWAS was performed based on the mixed linear model (PCA+K) using GAPIT packages in R software (Lipka et al, 2012;Lv et al, 2020; the R Core Team, 2019). The corrected Pvalues of 1.0e-3 and 3.16e-3 were used as the appropriate signi cance threshold for and II, respectively.…”

“…The development of wheat high-density SNP arrays (Sun et al 2020) including the Wheat 90K iSelect assay (Wang et al, 2014), 660K (Zhou et al, 2018), and 35K Wheat Breeder's assay (Allen et al, 2017) made it feasible to rapidly excavate genetic loci underlying complex traits in the whole wheat genome by genome-wide association studies (GWAS). In hexaploid wheat, GWAS has been widely used to identify many important genetic loci for regulating kinds of traits, including black point (Liu et al, 2017;Lv et al 2020), herbicide resistance (Shi et al 2020), Fusarium crown rot (Yang et al, 2019), and quality traits (Chen et al, 2019), etc.…”