Identification of genetic polymorphism in Mystus cavasius (Hamilton-Buchanan, 1822) from Gandhisagar reservoir and Bansagar reservoir, Madhya Pradesh, India

Garg, R. K.; Batav, Nitin; Singh, R. K.

doi:10.1016/j.jobaz.2013.06.001

Cited by 4 publications

(5 citation statements)

References 19 publications

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“…Total genomic DNA was extracted as protocol provided by [14], [15] with using some modifications. One hundred mg tissue sample was taken in pre-chilled eppendorf tube (1.5 ml capacity) and grinded tissue with the help of micro pestle within the tube.…”

Section: Methodsmentioning

confidence: 99%

Molecular insights into the genetic and haplotype diversity among four populations of Catla catla from Madhya Pradesh revealed through mtDNA cyto b gene sequences

Garg

Mishra

2018

Journal of Genetic Engineering and Biotechnology

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In the present investigation, the genetic structure of four populations of Catla catla, sequences of mitochondrial gene, cytochrome b (cyto b) from four populations were sequenced and analyzed. The sequences of mitochondrial regions revealed high haplotype diversity and low nucleotide diversity. The lowest 249 polymorphic sites and 0.00 parsimony informative sites were detected in populations of Fish Federation Pond (CCFFB) whereas highest 330 polymorphic sites and 56 parsimony informative sites were detected in populations of Narmada River (CCNRH) in the cyto b gene sequences in Catla catla populations. The twelve different haplotypes were detected among the four populations studied, lowest population specific haplotype as 2.00 was observed in Fish Federation Pond (CCFFB) and highest was in Population of Narmada River and Tighra reservoir. Sequencing of cyto b gene revealed 12 number of haplotypes (h) with haplotype (gene) diversity (Hd) 0.8736 and nucleotide diversity (π) 0.6474. These data clearly indicated that, feral/wild population showing highest values of polymorphisms, parsimony, haplotype diversity showing good, healthy habitat is lotic water (Narmada River) and lentic water body (Tighra reservoir). The results also concluded that the partial cyto b is polymorphic and can be a potential marker to determine ecological habitat based genetic differentiation among the populations.

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Section: Methodsmentioning

confidence: 99%

Molecular insights into the genetic and haplotype diversity among four populations of Catla catla from Madhya Pradesh revealed through mtDNA cyto b gene sequences

Garg

Mishra

2018

Journal of Genetic Engineering and Biotechnology

Self Cite

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“…Total genomic DNA was extracted by using phenol: chloroform: isoamyl-alcohol (25:24:1) method with some modifications [17][18][19][20]. 100 mg tissue sample was taken in pre-chilled eppendorf tube (1.5 ml capacity) and grinded tissue with the help of micro pestle within the tube.…”

Section: Extraction Of Genomic Dna From Tissues Samplesmentioning

confidence: 99%

Mitochondrial COI Gene Sequence Analyses of Puntius ticto and Compared with Seven Species of Genus Puntius of Family Cyprinidae: A Finding for Phylogenetic Positioning and DNA Barcoding as Model Study for Cryptic Species

Garg¹,

Dubey²,

Batav³

et al. 2017

J Proteomics Bioinform

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“…As Garg et al . () stated that extracted DNA with absorption ratio of A 260 /A 280 within 1.5–2.0 can be easily used for PCR, the extracted DNA from the DNeasy and NucleoSpin methods were considered satisfactorily for further PCR amplification.…”

Section: Resultsmentioning

confidence: 99%

“…Both DNeasy and NucleoSpin methods with silica membrane spin-column format produced similar range of DNA purity of 1.5-2.0 (Table 1) for all honey samples including the commercial. As Garg et al (2013) stated that extracted DNA with absorption ratio of A 260 /A 280 within 1.5-2.0 can be easily used for PCR, the extracted DNA from the DNeasy and NucleoSpin methods were considered satisfactorily for further PCR amplification.…”

Section: Purity Of Extracted Dna (A 260 /A 280 )mentioning

confidence: 99%

Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method

Kek

Chin

Tan

et al. 2018

Int J of Food Sci Tech

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Summary Four different DNA protocols were compared to obtain the best method for DNA extraction of bees in honey samples. The efficiency was evaluated in terms of DNA concentration and purity, PCR amplification capacity targeting mitochondrial 16S rRNA gene, and execution time. The most efficient DNA extraction method was selected using simple additive weighting (SAW) analysis. The DNeasy method with silica membrane spin‐column format scored highest in the final ranking of SAW compared to CTAB‐based, Wizard and NucleoSpin methods. It yielded extracted DNA with sufficient concentration (second highest), acceptable purity (1.5–2.0), amplifiable for 16S rRNA gene region, and required least extraction time. This recommended DNA extraction protocol suggests that genomic methodologies using a 150 bp amplicon of the 16S rRNA gene of bees enables identification of the entomological origin of honey.

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Identification of genetic polymorphism in Mystus cavasius (Hamilton-Buchanan, 1822) from Gandhisagar reservoir and Bansagar reservoir, Madhya Pradesh, India

Cited by 4 publications

References 19 publications

Molecular insights into the genetic and haplotype diversity among four populations of Catla catla from Madhya Pradesh revealed through mtDNA cyto b gene sequences

Molecular insights into the genetic and haplotype diversity among four populations of Catla catla from Madhya Pradesh revealed through mtDNA cyto b gene sequences

Mitochondrial COI Gene Sequence Analyses of Puntius ticto and Compared with Seven Species of Genus Puntius of Family Cyprinidae: A Finding for Phylogenetic Positioning and DNA Barcoding as Model Study for Cryptic Species

Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method

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