Identification of genomic regions associated with stay green in sorghum by testing RILs in multiple environments

Tao, Yuezhi; Henzell, R. G.; Jordan, David R.; Butler, Douglas N.; Kelly, Alison; McIntyre, C. Lynne

doi:10.1007/s001220051428

Cited by 172 publications

(145 citation statements)

References 17 publications

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“…Tx642 (B35) has been a useful source of staygreen for research and development of drought tolerant hybrids (Harris et al 2007;Tao et al 2000). It is an inbred BC 1 derivative line of IS12555 durra sorghum from Ethiopia, and varying dominance is exhibited by its genes regulating the staygreen trait (Xu et al 2000;Sanchez et al 2002).…”

Section: Germplasm Mapping Population and Evaluation Environmentsmentioning

confidence: 99%

“…This corresponds to the ability of a plant to maintain active photosynthetic processes with delayed senescence post-anthesis. In sorghum, staygreen has been associated with QTL that are stable across environments (Tao et al 2000;Haussmann et al 2002;Kassahun et al 2010). In general, four major QTL for staygreen individually exhibit different tolerance to droughtinduced senescence (Harris et al 2007;Kassahun et al 2010;Xu et al 2000;Rosenthal and Gerik 1991), as defined by the ability of some sorghum genotypes to stay green during post-anthesis water stress (Blum 2005;Tao et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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QTL mapping and loci dissection for leaf epicuticular wax load and canopy temperature depression and their association with QTL for staygreen in Sorghum bicolor under stress

et al. 2017

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Plant waxes and staygreen are distinct phenotypic traits that have been independently implicated in heat and drought tolerance among grasses. The association between these two traits has not been fully explored, which makes the exploitation of synergy between them difficult. This study assessed the association between QTL regulating the staygreen (Stg) trait in sorghum and those regulating epicuticular wax load (WL) and associated canopy temperature depression (TD). Using a recombinant inbred line (RIL) population derived from Tx642 and Tx7000, phenotypic data were collected in three replicated field trials and one greenhouse trial. High absolute TD generally corresponded to high WL. The r 2 of TD against WL was highest under non-stress conditions in the greenhouse while it was much larger in the cooler and irrigated field conditions compared to hotter, drier field trials. The genetic correlations between the two traits also followed this pattern. Composite interval mapping identified a total of 28 QTL, 15 of which had significant overlaps between different traits. Most of the wax QTL were associated with pre-anthesis drought tolerant Tx7000. However, one QTL for WL overlapped with a QTL for staygreen (Stg2) and was represented by a single, isolated marker near the centromeric region on the short arm of SBI-01. The marker is identified by a Cis-acting regulatory module and is part of a 2-kb multifunctional motif-rich region which includes core promoter and enhancer regions and transcription elements, including a droughtresponsive MYB binding site. We suggest that this QTL may be pleiotropic for important stress tolerance mechanisms regulating both staygreen and leaf wax in sorghum.

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Section: Germplasm Mapping Population and Evaluation Environmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

QTL mapping and loci dissection for leaf epicuticular wax load and canopy temperature depression and their association with QTL for staygreen in Sorghum bicolor under stress

et al. 2017

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“…Studies in sorghum have identified several QTLs for stay-green characters, which together explained more than half of the phenotypic variation in these qualities (Sanchez et al 2002). Multiple studies using crosses between different parental lines appear to have identified the same set of QTLs, on the basis of map location (Tuinstra et al 1997;Subudhi et al 2000;Tao et al 2000). The consistency of these QTLs among multiple sorghum crosses and in multiple environments indicates that allelic variants with large trait effects occur at relatively high frequencies at these loci among sorghum cultivars.…”

Section: Fig 1 Principles Of Qtl Mappingmentioning

confidence: 99%

Candidate Genes, Quantitative Trait Loci, and Functional Trait Evolution in Plants

Remington

Purugganan

2003

International Journal of Plant Sciences

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Two key characteristics of the neo-Darwinian synthesis in evolutionary biology have been its emphasis on the importance of mutations of small effect (micromutationism) and the view that studies of individual gene function shed relatively little light on evolutionary processes. Recent advances in molecular biology, however, have broken down many of the barriers between functional and evolutionary inquiry, opening the door to detailed studies of the genetic basis of functional trait evolution in plants. In this article, we review the insights into plant evolution that have been provided by molecular methods and address future research needs. Quantitative trait locus (QTL) mapping in crop and model plants has shown that individual loci often have large effects on trait variation, at variance with the micromutationist perspective. Evidence so far indicates that QTLs with large effects are also important in wild populations, underlying interspecific differences as well as intraspecific variation. Isolation of some of these QTLs, in particular for flowering time variation, has revealed a prominent role for regulatory genes known to function in regulation of flowering and exposed the complexity of regulatory processes. Preliminary evidence indicates that plant growth variation may be directly regulated rather than primarily the indirect result of selection on constituent processes. Future research should expand the number of traits that are intensively studied and make greater use of QTL mapping in wild plant taxa, especially those undergoing adaptive radiations, while continuing to draw on insights from model plants. Promising techniques include testing of candidate gene-trait associations in wild populations, genetic mapping in hybrid zones, and microarray analyses of gene expression.

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“…The trait is likely to be more easily manipulated using a marker-assisted breeding approach, using as selection criteria speciWc alleles at molecular loci linked to genomic regions contributing to the stay-green trait (i.e., quantitative trait loci = QTLs), identiWed in carefully managed, replicated, multi-environment tests. The work of diVerent groups has led to a better understanding of the inheritance of stay-green (Walulu et al 1994;van Oosterom et al 1996;Tuinstra et al 1997), to the mapping of QTLs associated with postXowering drought tolerance in sorghum (Tuinstra et al 1996(Tuinstra et al , 1998Crasta et al 1999;Tao et al 2000;Xu et al 2000b;Subudhi et al 2000;Kebede et al 2001;Sanchez et al 2002;Haussmann et al 2002) and identiWcation of markers linked to these QTLs (Hash et al 2003;Harris et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Stay-green expression in early generation sorghum [Sorghum bicolor (L.) Moench] QTL introgression lines

et al. 2009

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Reduced leaf senescence (stay-green) has been demonstrated to improve tolerance of post-Xowering moisture stress in grain sorghum. A number of quantitative trait loci (QTLs) associated with staygreen have been identiWed in sorghum, to facilitate transfer of this trait into adapted genetic backgrounds. This study reports initial evaluations, in both well watered and post-Xowering stress environments, following partial introgression (BC 2 F 3 /BC 1 F 4 generations) of four stable stay-green QTLs (StgB, Stg1, Stg3 and Stg4) from donor parent B35 to senescent variety R 16. The majority of the introgression lines had higher leaf chlorophyll levels at Xowering (a distinctive trait of the donor parent) and a greater percentage green leaf area during the latter part of grain Wlling, than did R 16, indicating that the stay-green QTLs were expressed phenotypically in the R 16 background. None of the QTL introgression lines achieved the same level of stay-green as B35, however.Maintenance of a greater relative green leaf area during the latter half of grain Wlling was related to a greater relative grain yield in two of three post-Xowering moisture deWcit environments in which the materials were evaluated (r 2 = 0.34 in 2004-2005 and r 2 = 0.76 in [2005][2006], as was a direct measure of leaf chlorophyll in one of the post-Xowering stress environments in which this was measured (r 2 = 0.42, P < 0.05). Thus the study provided useful evidence that the marker-assisted backcross transfer of staygreen QTLs from B35 into an adapted, but senescent background has the potential to enhance tolerance of post-Xowering drought stress in sorghum.

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Identification of genomic regions associated with stay green in sorghum by testing RILs in multiple environments

Cited by 172 publications

References 17 publications

QTL mapping and loci dissection for leaf epicuticular wax load and canopy temperature depression and their association with QTL for staygreen in Sorghum bicolor under stress

QTL mapping and loci dissection for leaf epicuticular wax load and canopy temperature depression and their association with QTL for staygreen in Sorghum bicolor under stress

Candidate Genes, Quantitative Trait Loci, and Functional Trait Evolution in Plants

Stay-green expression in early generation sorghum [Sorghum bicolor (L.) Moench] QTL introgression lines

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