Identification of Giardia lamblia Genotypes among Children in Tikrit City by Using Nested PCR

Abstract

The parasite Giardia lamblia is one of the most widespread intestinal protozoa that infects humans all over the world, causing giardiasis. It was first diagnosed in the duodenum and ileum in human digestive tract, where its effect is primarily on children. Giardia disease affects more than 280 million people every year worldwide. It is also one of the most important parasitic infections in many countries in terms of incidence and morbidity. 615 stool samples were collected from children attending Tikrit Teaching Hospital and private laboratories during the study pehriod to investigate the spread of G. lamblia among children, and the total infection rate was 14.30%. Where the rate of infecting children in the city was 11.54% of the total of 50 samples. Through the molecular examination in this study, the parasite G. lamblia, the target gene TPI triose phosphate isomerase was used to identify clusters of G. lamblia. All positive samples were positive with group B and no infection was registered with group A.

Giardia duodenalis Genotyping Not Linked to Clinical Symptomatology and Nutritional Status of School-Aged Children of Soledad and Galapa Municipality Schools, Atlántico, Colombia

“…Giardiasis in humans and animals is caused by genotypes A, B, and E. Genotype A is responsible for 99.2% of cases of giardiasis reported in humans and 33.8% of cases in animals such as domestic canines (Arruda-Fontenele et al, 2020;Hama Hasan et al, 2021;Pereira et al, 2021). Genotype A is also associated with the most significant clinical manifestations for health in children relative to those reported for genotype B (Sahagun et al, 2008;Atherton et al, 2013;Fantinatti et al, 2016;Arruda-Fontenele et al, 2020).…”