Identification of Glutathione Peroxidase (GPX) Gene Family in Rhodiola crenulata and Gene Expression Analysis under Stress Conditions

Abstract

Glutathione peroxidases (GPXs) are important enzymes in the glutathione-ascorbate cycle for catalyzing the reduction of H2O2 or organic hydroperoxides to water. GPXs play an essential role in plant growth and development by participating in photosynthesis, respiration, and stress tolerance. Rhodiola crenulata is a popular traditional Chinese medicinal plant which displays an extreme energy of tolerance to harsh alpine climate. The GPXs gene family might provide R. crenulata for extensively tolerance to environment stimulus. In this study, five GPX genes were isolated from R. crenulata. The protein amino acid sequences were analyzed by bioinformation softwares with the results that RcGPXs gene sequences contained three conserve cysteine residues, and the subcellular location predication were in the chloroplast, endoplasmic reticulum, or cytoplasm. Five RcGPXs members presented spatial and temporal specific expression with higher levels in young and green organs. And the expression patterns of RcGPXs in response to stresses or plant hormones were investigated by quantitative real-time PCR. In addition, the putative interaction proteins of RcGPXs were obtained by yeast two-hybrid with the results that RcGPXs could physically interact with specific proteins of multiple pathways like transcription factor, calmodulin, thioredoxin, and abscisic acid signal pathway. These results showed the regulation mechanism of RcGPXs were complicated and they were necessary for R. crenulata to adapt to the treacherous weather in highland.

Genome-Wide Characterization of Glutathione Peroxidase (GPX) Gene Family in Rapeseed (Brassica napus L.) Revealed Their Role in Multiple Abiotic Stress Response and Hormone Signaling

“…Further, GPX enzyme and GPX genes also contribute to several plant physiological, biochemical, and molecular mechanisms to withstand harsh environmental stresses [ 5 , 6 ]. In the recent past, GPX gene families have been reported in numerous crop plants, including five GPX genes in date palm ( Phoenix dactylifera L.) [ 51 ] and in Rhodiola crenulate [ 52 ]; six genes in cucumber ( Cucumis sativus L.) [ 53 ], watermelon ( Citrullus lanatus L.) [ 54 ], and Lotus japonicus [ 55 ]; seven genes in sorghum ( Sorghum bicolor L.) [ 19 ]; eight genes in Thellungiella salsuginea [ 56 ] and peach fruit ( Prunus persica L.) [ 57 ]; 13 genes in cotton ( Gossypium hirsutum L.) [ 58 ]; and 14 genes in bread wheat [ 59 ]. To the best of our knowledge, the GPX gene family was yet to be reported in rapeseed that responds to multiple stress conditions.…”

The clinical significance of glutathione peroxidase 2 in glioblastoma multiforme

“…GPX is composed of a series of isozymes (GPX1-8) that catalyze the reduction in H 2 O 2 or organic hydroperoxides to water or corresponding alcohols [ 11 , 20 ]. Among them, GPX2 is a gastrointestinal tract-enriched selenium-containing and GSH-dependent antioxidant enzyme [ 11 ].…”

Whole-Transcriptome RNA Sequencing Reveals the Global Molecular Responses and CeRNA Regulatory Network of mRNAs, lncRNAs, miRNAs and circRNAs in Response to Salt Stress in Sugar Beet (Beta vulgaris)

“…For example, choline monooxygenase ( Bv6_146100_wdro.t1 ) and betaine aldehyde dehydrogenase ( Bv5_116230_ntjn.t1 ), which are involved in the synthesis of betaine [ 45 ]; glutathione peroxidase ( Bv2_044150_kdwp.t1 ), ferrochelatase 2 ( Bv2_033330_nrat.t1 ) and soul heme-binding family protein ( Bv4_094190_wifn.t1 ) involved in heme biosynthesis in non-photosynthetic tissues and induced by oxidative stress in photosynthetic tissues to supply heme for defensive hemoproteins. Studies of rhodiola crenulata have shown that glutathione peroxidase can physically interact with specific proteins in multiple pathways which participate in photosynthesis, respiration and stress tolerance [ 46 ]. Further, alpha amylase ( Bv1_021820_ccxs.t1 ) was significantly up-regulated under salt stress, which suggests faster starch degradation by mesophyll cells.…”