Identification of goat cashmere and sheep wool by PCR-RFLP analysis of mitochondrial 12S rRNA gene

Geng, Rong; Yuan, Chao; Chen, Yulin

doi:10.3109/19401736.2012.710206

Cited by 10 publications

(3 citation statements)

References 20 publications

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“…Among these techniques, PCR-RFLP is considered as a highly discriminatory, reliable, and reproducibility method. Furthermore, the advantage of mitochondrial DNA-based PCR-RFLP analysis derives from the fact that there are many mitochondria per cell and many mitochondrial DNA molecules within each mitochondrion, making mtDNA a naturally amplified source of genetic variation [ 10 , 14 , 24 , 32 , 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

A PCR Method That Can Be Further Developed into PCR-RFLP Assay for Eight Animal Species Identification

Gao

Jin

Zhao

et al. 2018

Journal of Analytical Methods in Chemistry

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There are many PCR-based methods for animal species identification; however, their detection numbers are limited or could not identify unknown species. We set out to solve this problem by developing a universal primer PCR assay for simultaneous identification of eight animal species, including goat, sheep, deer, buffalo, cattle, yak, pig, and camel. In this assay, the variable lengths of mitochondrial DNA were amplified using a pair of universal primers. PCR amplifications yielded 760 bp, 737 bp, 537 bp, 486 bp, 481 bp, 464 bp, 429 bp, and 359 bp length fragments for goat, sheep, deer, buffalo, cattle, yak, pig, and camel, respectively. This primer pair had no cross-reaction with other common domestic animals and fish. The limit of detection varied from 0.01 to 0.05 ng of genomic DNA for eight animal species in a 20 µl PCR mixture. Each PCR product could be further digested into fragments with variable sizes and qualitative analysis by SspI restriction enzyme. This developed PCR-RFLP assay was sufficient to distinguish all targeted species. Compared with the previous published related methods, this approach is simple, with high throughput, fast processing rates, and more cost-effective for routine identification of meat in foodstuffs.

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Section: Resultsmentioning

confidence: 99%

A PCR Method That Can Be Further Developed into PCR-RFLP Assay for Eight Animal Species Identification

Gao

Jin

Zhao

et al. 2018

Journal of Analytical Methods in Chemistry

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“…DNA-analysis of mitochondrion DNA (mtDNA) extracted from animal hair or fibers through DNA amplification techniques (end point PCR or real-time PCR), is able to detect even a minute amount of these fibers [7]- [13]. With this technique, not only cashmere and wool would be distinguished but also their content in a cashmere-wool mixture can be quantified.…”

Section: Introductionmentioning

confidence: 99%

Identification and Quantitation of Cashmere (Pashmina) Fiber and Wool Using Novel Microchip Based Real-Time PCR Technology

Gill¹,

Gill²,

Slyadnev³

et al. 2018

JTST

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The textile industrial chain all over the world is facing a challenge of differentiating cashmere fiber from mixture of wool and other fibers in case cashmere stocks are adulterated with wool or other fibers. For identification of cashmere in such mixtures, the development of microchip based real-time PCR technology offers a very sensitive, specific, and accurate solution. The technology has been validated with cashmere and wool samples procured from distant farms, and from cashmere goats and sheep of different age and sex. Model samples with incremental raw cashmere or wool content were tested. The experimentally determined content was found to be comparable to the weighed content of the respective fibers in the samples. This technology may prove a cost cutter since it needs only 1.2 µl of the PCR reagent mix. It is substantially faster than traditional real-time PCR systems for being carried as miniature reaction volume in metal microchip. These features allow faster thermal equilibrium and thermal uniformity over the entire array of microreactors. For routine tests or in commercial set up, the microchips are available as ready-to-run with lyophilized reagents in its microreactors to which only 1 µl of the 10-fold diluted isolated DNA sample is added. The lyophilized microchips offer user-friendly handling in testing laboratories and help minimize human error.

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“…To date, a series of candidate genes have been identified for wool or cashmere traits in domestic animals (Zhou et al, 2011;Geng et al, 2012;Wang et al, 2012). Desmoglein 4 (DSG4) is an example of a candidate gene, whose role in the biological function of coat and skin has been demonstrated in many studies.…”

Section: Introductionmentioning

confidence: 99%

Desmoglein 4 diversity and correlation analysis with coat color in goat

Zhao

Cao

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Desmoglein 4 (DSG4) has an important role in the development of wool traits in domestic animals. The full-length DSG4 gene, which contains 3918 bp, a complete open-reading-frame, and encodes a 1040-amino acid protein, was amplified from Liaoning cashmere goat. The sequence was compared with that of DSG4 from other animals and the results show that the DSG4 coding region is consistent with interspecies conservation. Thirteen single-nucleotide polymorphisms (SNPs) were identified in a highly variable region of DSG4, and one SNP (M-1, G>T) was significantly correlated with white and black coat color in goat. Haplotype distribution of the highly variable region of DSG4 was assessed in 179 individuals from seven goat breeds to investigate its association with coat color and its differentiation among populations. However, the lack of a signature result indicates DGS4 haplotypes related with the color of goat coat.

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Identification of goat cashmere and sheep wool by PCR-RFLP analysis of mitochondrial 12S rRNA gene

Cited by 10 publications

References 20 publications

A PCR Method That Can Be Further Developed into PCR-RFLP Assay for Eight Animal Species Identification

A PCR Method That Can Be Further Developed into PCR-RFLP Assay for Eight Animal Species Identification

Identification and Quantitation of Cashmere (Pashmina) Fiber and Wool Using Novel Microchip Based Real-Time PCR Technology

Desmoglein 4 diversity and correlation analysis with coat color in goat

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