Identification of gram-negative bacteria of Bali cattle with repeat breeding cases on East Lombok, West Nusa Tenggara Province

Aminuddi, S. P.; Alpian, S. B.; Dita, P.; Kholik, Kholik

doi:10.1088/1742-6596/1430/1/012013

Cited by 6 publications

(6 citation statements)

References 5 publications

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“…These results are close to the results of the previous study, which stated that 33.33% (3/10) of E. coli were found in 10 isolates of Gram-negative bacteria in repeat breeder cattle in Aceh (9). Research on Bali cattle in Lombok also documented the presence of E. coli, Citrobacter freundii, and Proteus vulgaris bacteria in 4 samples of reproductive tract fluid from Bali cattle that experienced repeat breeder (18).…”

Section: Discussionsupporting

confidence: 89%

Characterization and phylogenetics of beta-lactamase Temoneira gene in Escherichia coli of the Bali cattle on Lombok island, Indonesia

Kholik

Srianto

Aulanni’am

et al. 2023

IJVS

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This study aims to determine the character and phylogenetics of the β-lactamase Temoneira (blaTEM) gene in Escherichia coli isolated from the reproductive tract of Bali cattle with repeat breeder cases. This study was conducted in June 2021 that used 16 female Bali cattle with repeat breeder cases to take their reproductive tract fluid using a plastic sheet gun. Isolation of Escherichia coli, using Eosin Methylene Blue Agar was identified by biochemical tests. Antibiotic susceptibility test on E. coli samples was carried out by disc diffusion method. The screening test for the presence of extended-spectrum β-lactamase was done using the double-disk approximation test method. Characterization of the blaTEM gene in E coli, using the PCR method and sequences of the blaTEM gene, were phylogenetically analyzed. The results showed that 4 E. coli isolates were obtained from 16 samples of reproductive tract fluid. The sensitivity test to antibiotics of 4 isolated samples of E. coli showed that 50% were resistant to penicillin and cefotaxime. Samples of E. coli resistant to penicillin and cefotaxime showed synergy results in the double-disk approximation test. The PCR results showed that the samples of E coli encoded the blaTEM gene which was located at the 560 bp position on gel electrophoresis. The phylogenetic tree analysis found that the blaTEM gene encoded by E. coli was related to E. coli encoding the blaTEM-206 gene. The character blaTEM gene in samples of E. coli showed the character of blaTEM-206 gene of extended-spectrum β-lactamase-producing E. coli. Escherichia coli from the reproductive tract of Bali cattle showed the character of E. coli S2.2-EK pEC-S2.2 blaTEM gene for class A broad-spectrum B-lactamase TEM-206 with a high degree of kinship.

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Section: Discussionsupporting

confidence: 89%

Characterization and phylogenetics of beta-lactamase Temoneira gene in Escherichia coli of the Bali cattle on Lombok island, Indonesia

Kholik

Srianto

Aulanni’am

et al. 2023

IJVS

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“…E. coli in methyl red and indole tests showed positive results and negative reactions for the Voges-Proskauer test and citrate, the results of these tests that distinguish them from other digestive tract bacteria (Cornelissen et al, 2013). This statement is corroborated by the research of Aminuddi et al (2020) who documented that E.coli isolated from the reproductive tract of Bali cattle ferment carbohydrates and produce gas. E. coli with the same biochemical results have also been isolated from anal swabs in bats, where E. coli ferments glucose and lactose (Kholik et al, 2019).…”

Section: E Coli Colonies Grown In Eosin Methylenementioning

confidence: 58%

“…Escherichia coli is used as an indicator because the AMR profile of E. coli almost reflects the use of antimicrobials in animals for food production (EFSA, 2011). Previous research data on the island of Lombok documented that E. coli had been isolated from Bali cattle with reproductive disorders in Central Lombok (Aminuddi et al, 2020). Gunawan et al (2018) have reported that among 94.4% of E. coli were isolated from 20 samples of Bali cattle feces in smallholder farms in Central Lombok, 61% of E. coli samples were resistant to Erythromycin and Tetracycline, and 16.7% of E. coli samples were resistant to Ciprofloxacin.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance in Escherichia coli Isolated from Feces of Bali Cattle With Reproductive Disorders

et al. 2021

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Antimicrobial resistance (AMR) has become a global issue in animal, human and environmental health. The AMR profile of Escherichia coli reflects the use of antibiotics in production animals. The purpose of this study was to determine the antibiotic resistance of Escherichia coli bacteria isolated from the feces of female Bali cattle with reproductive disorders. Feces samples were taken purposively using a swab on 4 rectums from 7 Bali cattle with reproductive disorders in June 2021 in Lando Village, East Lombok Regency. Escherichia coli samples were isolated on Eosin Methylene Blue Agar media and identified by biochemical tests. An antibiotic resistance test against Escherichia coli was carried out by the disk diffusion method. The antibiotics used in the test were Penicillin G 10 U, Oxytetracycline 30 g, Gentamicin 10 g, and Tetracycline 30 g, and Cefotaxime 30 g. The results of planting on Eosin Methylene Blue Agar media obtained 4 Escherichia coli which were successfully isolated from 4 samples of Bali cattle feces. Data on the level of Escherichia coli susceptibility level to various antibiotics were analyzed using the chi-square test. The results of the susceptibility test to antibiotics showed that 4 samples of Escherichia coli (100%) were resistant to Penicillin G, (25%) were resistant to Oxytetracycline, (25%) were resistant to Cefotaxime, and (100%) samples of Escherichia coli were sensitive to Gentamicin and Tetracycline. The chi-square test on the level of Escherichia coli susceptibility to various antibiotics was significant with p˂ 0.05 (p-value = 0.012). The results of this study have shown that Escherichia coli from Bali cattle feces experience multidrug resistance which later on might have an impact on human health and the environment.

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“…Ganguan penyakit sebagai akibat adanya infestasi penyakit yang dapat menggangu kesehatan. Peningkatan produksi dan reproduksi akan optimal, bila secara simultan disertai penyediaan pakan yang memadai dan pengendalian penyakit yang efektif [9]. Diantara sekian banyak penyakit hewan di Indonesia, penyakit parasit masih kurang mendapat perhatian dari para peternak [10].…”

Section: Pendahuluanunclassified

Prevalensi Cacing Fasciola Hepatica Pada Sapi Akseptor Program Upsus Siwab Di Kabupaten Muna

Zulkarnain

Aku

Rahmatullah

et al. 2021

JLAH

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UPSUS SIWAB adalah program pemerintah yang bertujuan untuk meningkatkan populasi sapi melalui perbaikan manajemen pemeliharaan dan reproduksi ternak. Salah satu kendala dalam pencapaian program tersebut adalah infeksi penyakit. Infestasi parasit cacing dilaporkan mampu menurunkan produktivitas ternak seperti penurunan bobot badan, daya kerja, kualitas daging, kulit, jeroan, dan terhambatnya pertumbuhan pada sapi muda serta berpotensi sebagai penular penyakit pada manusia (agen zoonosis). Penelitian ini bertujuan untuk mengetahui prevalensi cacing Fasciola hepatica (F. hepatica) pada sapi akseptor program UPSUS SIWAB di Kabupaten Muna. Sebanyak 270 sampel feses sapi dikoleksi dari sembilan kecamatan menggunakan metode random sampling. Variabel yang diamati, yaitu jenis telur cacing yang menginfestasi sapi akseptor menggunakan metode natif dan pembesaran mikroskop 100x (10x10). Selanjutnya, data dianalisis menggunakan persamaan prevalensi. Hasil studi menunjukkan bahwa tingkat prevalensi kejadian F. hepatica di Kabupaten Muna adalah 4,9%. Berdasarkan lokasinya, infestasi cacing F. hepatica dideteksi pada sapi akseptor yang tersebar di lima kecamatan, yaitu Kecamatan Parigi (0,74% ; 2/270), Lasapela (0,37% ; 1/270), Kabawo (2,59% ; 7/270), Watopute (0,37% ; 1/270) dan Tangkuno Selatan (0,74% ; 2/270). Faktor yang diduga berpengaruh terhadap variasi infeksi ini adalah sistem pemeliharaan ternak, keberadaan inang perantara dan tampungan air serta metode diagnose yang digunakan pada studi ini.

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Identification of gram-negative bacteria of Bali cattle with repeat breeding cases on East Lombok, West Nusa Tenggara Province

Cited by 6 publications

References 5 publications

Characterization and phylogenetics of beta-lactamase Temoneira gene in Escherichia coli of the Bali cattle on Lombok island, Indonesia

Characterization and phylogenetics of beta-lactamase Temoneira gene in Escherichia coli of the Bali cattle on Lombok island, Indonesia

Antibiotic Resistance in Escherichia coli Isolated from Feces of Bali Cattle With Reproductive Disorders

Prevalensi Cacing Fasciola Hepatica Pada Sapi Akseptor Program Upsus Siwab Di Kabupaten Muna

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