Identification of Gram-Positive Organisms

Colaninno, Peter M.

doi:10.1201/b17871-7

Cited by 2 publications

(2 citation statements)

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“…High salt in mannitol salt agar allows few organisms that tolerate salt to grow such as Staphylococci. To be sure in identification of S. aureus, mannitol fermenter colonies on mannitol salt agar was confirmed by tube coagulase test and not the slide method which detects a less specific clumping factor which may be positive in strains other than S. aureus (1,20,41) . Different carrier figures of S. aureus were reported.…”

Section: Discussionmentioning

confidence: 99%

“…Confirmation of isolated S. aureus was performed using Gram stain, tube coagulase test (20,21) . S. aureus isolates were sub-cultured on less salt coating media such as nutrient agar before it was subjected to antimicrobial susceptibility test using Kirby-Bauer method (22) , using five antimicrobial agents; Amoxicillin/Calvulinic acid 30 mcg, Oxacillin 1mcg, Vancomycin 30 mcg, Fusidic acid 10 Mcg, Methicillin 10 mcg (Bioanalyse Ltd., Turkey).…”

Section: Methodsmentioning

confidence: 99%

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Nasal Carriers of Methicillin-Resistant Staphylococcus Aureus (Mrsa), Infection Safety and Hand Hygiene Among Health Workers in Sulaimani City

Fathi¹,

Omer²,

Mohammed³

2013

JSMC

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BackgroundStaphylococcus aureus, a well known constant human pathogen that also exploit human as carrier to new hosts when inhabiting area like nose and skin. Over years strains had emerged with ability to resist the effect of several antimicrobial agents. The ability to resist the methicillin caused by different genetic backgrounds was one of the problems in the fight against these organisms. ObjectivesThis study was aimed to isolate and identify Methicillin resistant Staphylococcus aureus (MRSA) inhabiting the nose of health workers in the main hospitals in Sulaimani city and to identify the susceptibility of these strains to several antimicrobial agents, also to inquire about daily infection safety and hand hygiene practice of the health workers. MethodsTo those who agreed to participate in the study, questionnaires were documented. Nasal swabs were obtained and isolation of Staphylococci was done on selective media, then S. aureus was confirmed and subjected to antimicrobial susceptibility using disk diffusion method. ResultsThe participants were grouped in to nine occupational groups. Gloves were always used by 30.5% of the participants, not used in 18.8% and the other 50.6% were selective in using gloves. Hand cleansing with antiseptic were practiced in 31.7% while face mask were used by 18.4%. Hand washing during work in health facility were not practiced in 58.6%, 1-10 times hand washing was reported in 20.4%, 11-20 times hand washing in 10.8% and more than twenty time washing in 10%. Forty five out of 249 nasal swabs yielded S. aureus making the carrier rate of 18% while methicillin-resistant S. aureus rate was 5.6% among the participants. ConclusionHand hygiene practice and infection safety were suboptimal in our health facilities. Nasal carrier of S. aureus was 18% in health workers while MRSA strains were isolated in 5.6% of the health workers. Prompt measure for hand hygiene must be implemented urgently to control transmission of S. aureus and to limit development of antimicrobial resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Nasal Carriers of Methicillin-Resistant Staphylococcus Aureus (Mrsa), Infection Safety and Hand Hygiene Among Health Workers in Sulaimani City

Fathi¹,

Omer²,

Mohammed³

2013

JSMC

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Oral Findings and Microflora in Type Ii Diabetes Mellitus in Sulaimani City

Abass¹,

Omer²

2011

JSMC

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Background The effects of diabetes mellitus on human include long term dysfunction and failure of various organs. A number of oral diseases and disorders have been associated with diabetes mellitus. The susceptibility to periodontal disease often called the "sixth complication of diabetes mellitus" is the most common oral complication of diabetes. The oral cavity is comprised of many surfaces, each coated with a plethora of different bacteria, some of which have been implicated in oral diseases such as caries and periodontitis. Objective This study was conducted to determine the most common microorganisms inhabiting the oral cavity of diabetic individuals in comparison to non diabetics, to determine the relation of oral microflora to oral conditions in diabetes and determine the susceptibility of oral microflora to common antimicrobial agents. Methods A case control study on two hundred persons with type II diabetes mellitus and fifty control cases. Oral findings were documented based on physical examination, microbial identification was based on culture methods and various identification tests. Antimicrobial susceptibility was performed according to Kirby- Bauer method. Results The oral findings in persons with type II diabetes mellitus showed higher occurrence of gingivitis, periodontitis, dental pain, xerostomia, taste disturbance, palatal ulceration and oral candidiasis in comparison with person without diabetes. Microorganisms studied by culture-dependent methods showed more bacterial isolates in diabetic groups of many stains such as of Bacillus species, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Streptococcus pneumoniae, Streptococcus pyogenes, Viridans streptococci, Branhamella catarrhalis, Escherichia coli, Stomatococcus, Veillonella and Candida albicans. The bacterial isolate susceptibility to antimicrobial agents showed wide resistance to several commonly used antimicrobial agents in dental practice and intermediate response was shown to be arising to some antimicrobial agents. Conclusion Various oral and dental problems such as periodontitis and dental loss was observed in diabetics, also more microbial isolates were documented from diabetics with a notable shift to more virulent species. Most of the isolated organism were resistant to several commonly antimicrobial agents in use. Oral problems may reflect on the increasing oral population of certain bacterial species and this will further complicate their oral problems.

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Identification of Gram-Positive Organisms

Cited by 2 publications

References 58 publications

Nasal Carriers of Methicillin-Resistant Staphylococcus Aureus (Mrsa), Infection Safety and Hand Hygiene Among Health Workers in Sulaimani City

Nasal Carriers of Methicillin-Resistant Staphylococcus Aureus (Mrsa), Infection Safety and Hand Hygiene Among Health Workers in Sulaimani City

Oral Findings and Microflora in Type Ii Diabetes Mellitus in Sulaimani City

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