Identification of Group A Porcine Rotavirus Strains Bearing a Novel VP4 (P) Genotype in Italian Swine Herds

Martella, Vito; Ciarlet, Max; Bányai, Krisztiàn; Lorusso, Eleonora; Arista, Serenella; Llombart‐Bosch, Antonio; Pezzotti, Giovanni; Decaro, Nicola; Cavalli, Alessandra; Lucente, Maria Stella; Corrente, Marialaura; Elia, Gabriella; Camero, Michele; Tempesta, Maria; Buonavoglia, Canio

doi:10.1128/jcm.02262-06

Cited by 78 publications

(85 citation statements)

References 43 publications

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“…It is reported in both symptomatic as well as asymptomatic pigs of all age groups (Morin et al 1990;Kim et al 1999;Collins et al 2008;Marthaler et al 2013). Published reports based on the sequence analysis of VP7 and VP4 genes from RVC reveal the circulation of a CONTACT Yashpal Singh Malik malikyps@ivri.res.in genetically heterogeneous group of isolates worldwide (Martella et al 2007;Jeong et al 2015;Moutel ıkova et al 2015). Based on the VP6 sequence analysis, a total of 11 I genotypes are confirmed in RVC (Suzuki et al 2014;Moutel ıkova et al 2015), whereas based on VP4 gene, P1-P8 types, and on VP7 gene, G1-G11 types are identified (Jeong et al 2015;Moutel ıkova et al 2015) and approved by the RCWG.…”

Section: Introductionmentioning

confidence: 99%

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Kattoor

Saurabh

Malik

et al. 2017

Veterinary Quarterly

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Background: Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog. Objective: To demonstrate the presence of RVC in Indian swine population and characterization of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes. Methods: A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern. To characterize the RVC strains, VP6 gene and NSP4 and NSP5 genes were amplified, sequenced and analyzed. Results: Based on VP6 gene specific diagnostic RT-PCR, the presence of RVC was confirmed in 12.0% (13/108) piglet fecal specimens. The nucleotide sequence analysis of VP6 gene, encoding inner capsid protein, from selected porcine RVC (PoRVC) strains revealed more than 93% homologies to human RVC strains (HuRVC) of Eurasian origin. These strains were distant from hitherto reported PoRVCs and clustered with HuRVCs, owning I2 genotype. However, the two non-structural genes, i.e. NSP4 and NSP5, of these strains were found to be of swine type, signifying a re-assortment event that has occurred in the Indian swine population. Conclusion:The findings indicate the presence of human-like RVC in Indian pigs and division of RVC clade with I2 genotype into further sub-clades. To the best of our knowledge, this appears to be the first report of RVC in Indian swine population. Incidence of human-like RVC VP6 gene in swine supports its subsequent zoonotic prospective.

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Section: Introductionmentioning

confidence: 99%

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Kattoor

Saurabh

Malik

et al. 2017

Veterinary Quarterly

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“…Results with the RT-PCR method showed excellent correlation with results generated by serotype specific monoclonal antibodies [Taniguchi et al, 1992] and therefore, G types are often referred to as G serotype, even if determined by molecular means. Unlike the G serotype, concordance between the assignment of VP4 serotype and VP4 genotype are inconsistent [Padilla-Noriega et al, 1998] and therefore, a dual P classification system has been adopted where P genotypes are written between square brackets (P[8]) and P serotypes are written normally (P1a) [Gentsch et al, 2005] [Hoshino and Kapikian, 2000;Gentsch et al, 2005;Khamrin et al, 2007;Martella et al, 2007;Parra et al, 2007;Steyer et al, 2007a].…”

Section: Introductionmentioning

confidence: 99%

The detection and molecular characterization of human G12 genotypes in South Africa

Page

Beer

Seheri

et al. 2008

Journal of Medical Virology

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The last decade has seen an increase in the detection of rotavirus strains other than G1–G4 emerging or even predominating in some settings. The performance of the current rotavirus vaccines against unusual or rare circulating rotavirus serotypes cannot be predicted and continuous monitoring of wild type rotaviruses will remain a priority. Routine molecular rotavirus surveillance conducted in the Gauteng Province, South Africa during 2004, resulted in the detection of strains that could not typed using standard G specific genotyping primers. Sequencing of the first round amplicons revealed 19 serotype G12P[6] strains and one G12P[8] strain. Phylogenetic analyses of the G12 strains indicated that these strains are probably a recent introduction into South Africa and emerged from a strain related to the Indian isolate ISO‐5. The association of the South African G12s with the P[6] genotype may suggest a mechanism for unusual strains to become more ecologically suited to local population transmission dynamics. This is the first report of serotype G12 strains on the African continent and continued surveillance will be required to track the emergence of G12 strains in Africa. J. Med. Virol. 81:106–113, 2009. © 2008 Wiley‐Liss, Inc.

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“…An isolate was propagated onto African green monkey kidney (MA-104) cells as described [21][22][23]. Evidence of viral replication was checked both by the appearance cytopathic effect and indirect immunofluorescence test, using a rabbit hyperimmune serum raised against a group A rotavirus (strain Bo/A-125/96 P7 [5],G6).…”

Section: Virus Isolationmentioning

confidence: 99%

“…Subsequently, the supernatants were centrifuged for 8 h at 85,000g on a 30% sucrose layer in SW28 rotor (Beckman Coulter, Inc., Fullerton, CA). After digestion of viral pellet with 1% sodium-dodecyl-sulfate (SDS) and proteinase K 1 lg/ml (Sigma-Aldrich, Milano, Italy), viral dsRNA was extracted and the e-type was visualized as described [21][22][23].…”

Section: Virus Isolationmentioning

confidence: 99%

“…In recent years, epidemiological surveillance to monitor the appearance of novel rotavirus antigenic types has intensified throughout the world, yielding evidence for the increasing antigenic diversity of group A rotaviruses [21][22][23]. Although the number of characterized OVR strains is small, OVRs seem to represent a diverse group, suggesting that sheep may interchange rotaviruses readily.…”

Section: Introductionmentioning

confidence: 99%

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Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

Ciarlet

Hoffmann

Lorusso³

et al. 2008

Virus Genes

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An ovine rotavirus (OVR) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in Zaragoza, Spain, and the VP4, VP7, VP6, NSP4, and NSP5/NSP6 genes were subsequently characterized molecularly. Strain OVR762 was classified as a P[14] rotavirus, as the VP4 and VP8* trypsin-cleavage product of the VP4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the P11[14] human rotavirus (HRV) strain PA169, isolated in Italy. Analysis of the VP7 gene product revealed that OVR762 possessed G8 serotype specificity, a type common in ruminants, with the highest degree of aa identity (95-98%) shared with serotype G8 HRV, bovine rotavirus, and guanaco (Lama guanicoe) rotavirus strains. Moreover, strain OVR762 displayed a bovine-like NSP4 (genotype E2) and NSP5/NSP6 (genotype H3), and a VP6 genotype I2, as well as a long electropherotype pattern. This is the first report of a lamb rotavirus with P[14] and G8 specificities, providing additional evidence for the wide genetic and antigenic diversity of group A rotaviruses.

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Identification of Group A Porcine Rotavirus Strains Bearing a Novel VP4 (P) Genotype in Italian Swine Herds

Cited by 78 publications

References 43 publications

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

The detection and molecular characterization of human G12 genotypes in South Africa

Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

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