Identification of Group G Streptococcal Isolates from Companion Animals in Japan and Their Antimicrobial Resistance Patterns

Tsuyuki, Yuzo; Kurita, Goro; Murata, Yoshiteru; Goto, Mieko; Takahashi, Takashi

doi:10.7883/yoken.jjid.2016.375

Cited by 23 publications

(16 citation statements)

References 24 publications

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“…In 2015, we compared the species/subspecies identification methods of 16S rRNA sequencing with the results obtained by matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF MS) and an automated biochemical identification method (Vitek 2 system with the Gram positive ID Card) using 72 group G streptococcal strains (13). There were discrepancies between the 16S rRNA sequencing results and the identification data from both MALDI-TOF MS and the automated biochemical assay.…”

Section: Discussionmentioning

confidence: 99%

“…Two emm types (stC9431.0 and stG6792.3) with stC1929.1 (original type from a skin wound of a house cat) were observed in the 2015 S. dysgalactiae subsp. equisimilis strain (13). The stC9431.0 was recovered from sterile human specimens in the USA (25), and stG6792.3, stG245.0, and stG485.0 are the major isolate types found in human patients in Japan (14).…”

Section: Discussionmentioning

confidence: 99%

“…Clinical specimens, submitted by veterinary practitioners, along with request sheets including the information of companion animals nationwide, were immediately sent to the Sanritsu Zelkova Veterinary Laboratory to examine them for causative bacterial agents. These specimens were taken from diseased companion animals, which visited either clinics or hospitals during the study period of April to May 2017, with significant symptoms or signs found either by pet owners or the veterinary clinicians (13). Specimens were derived from either sterile (blood, joint fluid, etc.)…”

Section: Collection Of Bacterial Isolates and Background Of Companionmentioning

confidence: 99%

“…Determination of species identification: DNA was extracted from the β-hemolytic streptococcal isolates by suspending in TE buffer and boiling at 97°C for 10 min as previously described (14). We identified the βhemolytic streptococcal strains at the species-level based on 16S rRNA sequencing data (13,15) (Table 1). The isolates identified were unambiguously identified, with ≥ 98.7% similarity to the 16S rRNA sequence of the corresponding strain.…”

Section: Collection Of Bacterial Isolates and Background Of Companionmentioning

confidence: 99%

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Species Identification of β-Hemolytic Streptococci from Diseased Companion Animals and Their Antimicrobial Resistance Data in Japan (2017)

et al. 2019

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This study aimed to identify the species and assess the antimicrobial resistance (AMR) of β-hemolytic streptococci isolated from companion animals in Japan. Strains were isolated from clinical specimens of 131 companion animals that exhibited symptoms in April-May 2017. We identified strains by 16S rRNA sequencing and assessed their antimicrobial susceptibility using the broth microdilution method. AMR genes erm(A)-erm(B)-mef(A) and tet(M)-tet(O)-tet(K)-tet(L)-tet(S) in all isolates were amplified by PCR. 16S rRNA sequencing identified β-hemolytic streptococcal species as Streptococcus canis (n = 117, 89.3%), S. agalactiae (n = 7), S. dysgalactiae subsp. equisimilis (n = 5), S. dysgalactiae subsp. dysgalactiae (n = 1), and S. equi subsp. zooepidemicus (n = 1). Overall AMR rates were 39.7% for minocycline, 19.8% for erythromycin, and 17.6% for clindamycin, with a minimum inhibitory concentration (MIC 90) of > 4, > 2, and > 1 μg/mL, respectively. AMR genotyping showed the presence of single or mixed types: erm(B)-mef(A) and tet(M)-tet(O)-tet(L)-tet(S). There was a significant relationship between tetracycline-resistance genotypes and open pus/skin-derived specimens. These observations identify some unique features of β-hemolytic streptococcal isolates from companion animals in Japan, such as the dominant isolation of S. canis and resistance to tetracycline, macrolide, and lincosamide antibiotics, in terms of species identification and AMR properties.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Collection Of Bacterial Isolates and Background Of Companionmentioning

confidence: 99%

Section: Collection Of Bacterial Isolates and Background Of Companionmentioning

confidence: 99%

See 2 more Smart Citations

Species Identification of β-Hemolytic Streptococci from Diseased Companion Animals and Their Antimicrobial Resistance Data in Japan (2017)

et al. 2019

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“…During previous studies (18,19), we collected 68 and 117 S. canis isolates in 2015 and 2017, respectively, from diseased companion animals along, with the animal information. We identified the S. canis strains at the species level based on 16S rRNA sequencing data (18,19). We also validated the accuracy of the identification through…”

Section: Selection Of S Canis Strainsmentioning

confidence: 99%