Identification of guanosine diphosphate derivatives of d-xylose, d-glucose and d-galactose in mature strawberry leaves

Abstract

The nucleotides from a trichloroacetic acid extract of mature strawberry leaves were separated into ten main fractions by chromatography on a Dowex 1 (formate form) column with ammonium formate as the eluting agent. One of these fractions, which was suspected to contain not only ADP but also GDP-sugars, was separated into a number of subfractions by further chromatography on a Dowex 1 (formate form) column with the formic acid system as the eluting agent. One of these subfractions was identified from its ultraviolet spectra, from its position on the two ion-exchange columns and by thin-layer chromatography as a GDP-sugar. Mild acid hydrolysis gave GDP and a mixture of sugars. The sugars, after a preliminary separation on a paper chromatogram, were identified by an isotope-dilution method. The sugars were condensed with sodium [(14)C]cyanide, the [(14)C]nitriles were hydrolysed and one of the epimeric acids was isolated, either as lactone or amide, by co-crystallization with a non-radioactive carrier. This method distinguishes between enantiomorphic sugars. d-Mannose, d-xylose, d-glucose and d-galactose were present in the proportions 40:10:1:1 respectively. The total amount of the GDP-sugars was approx. 0.1mumole/100g. of fresh leaves.

Biosynthesis of Oligosaccharides and Polysaccharides in Plants

“…Although GDP-D-glucose has been reported from strawberry leaves (83), it has not been found in mung beans. The failure to detect it may be attributed to the low level of this sugar nucleotide in the plant.…”

Acid-soluble phosphorus compounds in mammalian semen

“…The elution of the nucleotides was followed by measurement of E260, and 0.1 ml portions were taken from the fractions (2.75 ml) for determinations of phosphorus and radioactivity. Fractions representing separate peaks were pooled, freeze-dried and freed from ammonium formate as described by Selvendran & Isherwood (1967). Material from the isolated peaks was rechromatographed on columns (0.6cmx 10cm) of Dowex AGI (X2) resin at 20ml/h with the formic acid system of Hurlbert et al (1954) modified as described above (volume of mixing chamber 25ml).…”

Separation and identification of soluble nucleotides in cambial and young xylem tissue of Larix decidua Mill

“…UDP-arabinose and UDP-galactose may be concerned with biosynthesis ofarabinogalactans.Twointerestingnucleotidemonosaccharides that were present in the primary as well as the secondary wall fractions are UDPand ADPfructose. ADP-fructose has not previously been isolated from plant tissue, but both GDP-fructose (Selvendran & Isherwood, 1967) and UDP-fructose (Gonzalez & Pontis, 1963;Umemura, Nakamura & Funahashi, 1967;Brown & Mangat, 1967;Sandermann & Grisebach, 1968) have been found in higher plants. In two instances the latter has been isolated from tissue where it is thought to play a role in the formation of fructosans (dahlia tubers and Jerusalem artichoke).…”