IntroductionThis study addresses the urgent need for non-destructive identification of commercially valuable Dalbergia species, which are threatened by illegal logging. Effective identification methods are crucial for ecological conservation, biodiversity preservation, and the regulation of the timber trade.MethodsWe integrate Visible/Near-Infrared (Vis/NIR) Hyperspectral Imaging (HSI) with advanced machine learning techniques to enhance the precision and efficiency of wood species identification. Our methodology employs various modeling approaches, including Principal Component Analysis (PCA), Partial Least Squares Discriminant Analysis (PLS-DA), Support Vector Machine (SVM), and Convolutional Neural Networks (CNN). These models analyze spectral data across Vis (383–982 nm), NIR (982–2386 nm), and full spectral ranges (383 nm to 2386 nm). We also assess the impact of preprocessing techniques such as Standard Normal Variate (SNV), Savitzky-Golay (SG) smoothing, normalization, and Multiplicative Scatter Correction (MSC) on model performance.ResultsWith optimal preprocessing, both SVM and CNN models achieve 100% accuracy across NIR and full spectral ranges. The selection of an appropriate wavelength range is critical; utilizing the full spectrum captures a broader array of the wood's chemical and physical properties, significantly enhancing model accuracy and predictive power.DiscussionThese findings underscore the effectiveness of Vis/NIR HSI in wood species identification. They also highlight the importance of precise wavelength selection and preprocessing techniques to maximize both accuracy and cost-efficiency. This research contributes substantially to ecological conservation and the regulation of the timber trade by providing a reliable, non-destructive method for identifying threatened wood species.