Identification of haemocytes and histological examination of gills of the spiny oyster Spondylus gaederopus (Linnaeus, 1758)

Fabrello, Jacopo; Pagano, Maria; Arrigo, Federica; Ciscato, Maria; Boldrin, Francesco; Giacobbe, Salvatore; Porcino, Caterina; Biriglia, Marilena; Guerrera, Maria Cristina; Germanà, Antonino; Faggio, Caterina; Matozzo, Valerio

doi:10.1016/j.fsi.2022.09.013

Cited by 15 publications

(3 citation statements)

References 37 publications

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“…Indeed, they have chemotaxis capability, migrating toward attractive substances (Fawcett & Tripp, 1994), and they produce enzymes and antimicrobial peptides (Cheng & Chen, 2000). The presence of two cell populations with spreading behavior, confirmed by electron microscopy analysis (TEM), indicate a shared phagocytic capability suggesting that P. imbricata radiata is particularly efficient in self‐defense (Fabrello et al, 2022; Mosca et al, 2013). Investigation on some biochemical aspects of phagocytosis in P. fucata (Wang et al, 2011) seem to confirm this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

“…Such results are confirmed and implemented by the present investigation, in agreement with the hypothesis that physiological responses to environmental stresses in the P. imbricata specie complex, especially at the haemocyte level, may be valuable tools in monitoring programs, eventually coupled with other proposed targets, as gill ultrastructure (Hamed et al, 2014). Such an option, which has been recently proposed for another Pteriomorphia, that is, the Mediterranean endemic Spondylus gaederopus L. 1758 (Fabrello et al, 2022), for Pinctada ssp. would have the advantage of employing a species, or a species complex, worldwide occurring in warm and temperate environments, further than more abundant and more resistant in stressed environments.…”

Section: Discussionmentioning

confidence: 99%

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A first insight into haemocytes of Pinctada imbricata radiata: A morpho‐functional characterization

Pagano

Fabrello

Multisanti

et al. 2022

Microscopy Res & Technique

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The pearl oyster Pinctada imbricata radiata (Leach, 1814), from the Pacific Ocean, was one of the first species to reach via Suez the Mediterranean, colonizing the eastern basin and recently spreading to the western. The species showed to be able to adapt to a wide range of climatic, hydrological, and ecological conditions. Since 2000 it reached the Strait of Messina, where is now infesting the transitional waters of the oriented natural reserve “Laguna di Capo Peloro.” Due to such resistance and adaptation ability, various assays were performed. Haemocyte morpho‐functional aspects were evaluated in haemolymph samples fixed with 1% and 2% glutaraldehyde for optical and electron microscopy (TEM). The following assays were carried out: cell characterization using several dyes, detection of intra‐ and extracellular lipids, the capability of phagocytosis using the yeast Saccharomyces cerevisiae and to produce superoxide anion (O2−). Detection of several enzymes, such as acid and alkaline phosphatase, arylsulfatase, chloro‐acetylesterase and β‐glucuronidase was also assessed. Cell count was demonstrated to be abundant with a mean of 8.263 × 106 mm2 ± 0.935 × 106 (SD). Two main cell populations were noticed: granulocytes and hyalocytes, both competent for phagocytosis, to produce O2−, and characterized by lipids. Based on the granule analysis, enzymatic activity was also demonstrated. The observations under TEM confirmed all the results obtained. This study supports the hypothesis that P. imbricata radiata can be usefully employed as a model organism in environmental biomonitoring. Moreover, since the species represent potential threats to native species and ecosystems, further insights into its biological adaptations in invaded ecosystems are recommended.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A first insight into haemocytes of Pinctada imbricata radiata: A morpho‐functional characterization

Pagano

Fabrello

Multisanti

et al. 2022

Microscopy Res & Technique

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“…In fish, these structures are a type of modified endothelial cell defining the blood spaces within the gill lamellae, regulating the blood flow to the respiratory surface by contraction and preventing either collapse of the lamellae or too wide separation of the lacunar space (Rankin & Maetz, 1971;Wilson & Laurent, 2002). Pillar cells have also been found in mussels (Fabrello et al, 2022;Nakao, 1975;Stalin et al, 2011). Some authors refer to these cells as trabecular cells, and despite their differences from fish pillar cells, the function of regulating hemolymph flow is similar (Nakao, 1975).…”

Section: Mytilus Galloprovincialis Respiratory Apparatusmentioning

confidence: 99%

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Impellitteri,

Briglia,

Porcino

et al. 2024

Microscopy Res & Technique

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In recent years, the presence of pharmaceuticals and microplastics (MPs) in aquatic ecosystems has raised concerns about their environmental impact. This study explores the combined effects of caffeine, a common pharmaceutical pollutant, and MPs on the marine mussel Mytilus galloprovincialis. Caffeine, at concentrations of 20.0 μg L−1, and MPs (1 mg L−1, 35–50 μm size range), was used to mimic real‐world exposure scenarios. Two hundred M. galloprovincialis specimens were divided into four groups: caffeine, MPs, Mix (caffeine + MPs), and Control. After a two‐week acclimation period, the mollusks were subjected to these pollutants in oxygen‐aerated aquariums under controlled conditions for 14 days. Histopathological assessments were performed to evaluate gill morphology. Cellular volume regulation and digestive gland cell viability were also analyzed. Exposure to caffeine and MPs induced significant morphological changes in M. galloprovincialis gills, including cilia loss, ciliary disk damage, and cellular alterations. The chitinous rod supporting filaments also suffered damage, potentially due to MP interactions, leading to hemocyte infiltration and filament integrity compromise. Hemocytic aggregation suggested an inflammatory response to caffeine. In addition, viability assessments of digestive gland cells revealed potential damage to cell membranes and function, with impaired cell volume regulation, particularly in the Mix group, raising concerns about nutrient metabolism disruption and organ function compromise. These findings underscore the vulnerability of M. galloprovincialis to environmental pollutants and emphasize the need for monitoring and mitigation efforts.Research Highlights The synergy of caffeine and microplastics (MPs) in aquatic ecosystems warrants investigation. MPs and caffeine could affect gill morphology of Mytilus galloprovincialis. Caffeine‐exposed cells had lower viability than the control group in the NR retention test. MPs and mix‐exposed cells struggled to recover their volume.

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Anodonta cygnea, a freshwater swan mussel, exposed to diazinon: toxicity thresholds in behaviour and physiology

et al. 2023

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Identification of haemocytes and histological examination of gills of the spiny oyster Spondylus gaederopus (Linnaeus, 1758)

Cited by 15 publications

References 37 publications

A first insight into haemocytes of Pinctada imbricata radiata: A morpho‐functional characterization

A first insight into haemocytes of Pinctada imbricata radiata: A morpho‐functional characterization

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Anodonta cygnea, a freshwater swan mussel, exposed to diazinon: toxicity thresholds in behaviour and physiology

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