1990
DOI: 10.1182/blood.v76.6.1139.bloodjournal7661139
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Identification of hematopoietic progenitors of macrophages and dendritic Langerhans cells (DL-CFU) in human bone marrow and peripheral blood

Abstract: Colonies of cells with distinctive dendritic appearance were observed in methylcellulose cultures of human bone marrow and peripheral blood mononuclear cells (PBMC). Such cells appeared alone in colonies of less than 50 cells, together with macrophages in mixed colonies and also within clusters of T lymphocytes at high culture cell numbers. The morphologic resemblance to lymphoid dendritic cells was confirmed by electron microscopy and the cells were distinguished from macrophages by immunoenzymatic and immuno… Show more

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Cited by 40 publications
(57 citation statements)
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“…Lymphoid DC grow independently of GM-CSF in colony assays 22 and in GM-CSF -/mice. 26 However, GM-CSF, in combination with other cytokines, induces commitment of a common myeloid/DC precursor to the DC lineage 19 and induces differentiation of blood monocytes to give cells thought to be myeloid DC. 20 Further evidence to support lymphoid versus myeloid lineage distinction comes from evidence that rel-B expression is a myeloid specific marker 27 and Ikaros expression is a lymphoid DC specific marker.…”
Section: Dendritic Cell Subsets With Distinct Functional Capacitymentioning
confidence: 99%
See 1 more Smart Citation
“…Lymphoid DC grow independently of GM-CSF in colony assays 22 and in GM-CSF -/mice. 26 However, GM-CSF, in combination with other cytokines, induces commitment of a common myeloid/DC precursor to the DC lineage 19 and induces differentiation of blood monocytes to give cells thought to be myeloid DC. 20 Further evidence to support lymphoid versus myeloid lineage distinction comes from evidence that rel-B expression is a myeloid specific marker 27 and Ikaros expression is a lymphoid DC specific marker.…”
Section: Dendritic Cell Subsets With Distinct Functional Capacitymentioning
confidence: 99%
“…CD34 + BM or cord blood stem cells after culture with GM-CSF, stem cell factor (SCF), IL-4 and TNF-α. 19 The cells derived by these two methods are thought to resemble 'myeloid-like' DC, because they are derived from a precursor cell that can also give rise to macrophages or that can turn into macrophages when cultured in a different growth factor environment, such as addition of macrophage colony stimulating factor (M-CSF). 20 A third method is to generate DC from lymphocyte precursors cultured in vitro in a cocktail of growth factors.…”
mentioning
confidence: 99%
“…None have a lymphocytic marker. After remission of several hemopathies after chemotherapy, there is in bone marrow and in blood a population of CDla-I-cells which share morphologic and phenotypic features between LC and macrophages [40]. These cells are probably common precursors of LC and macrophages.…”
Section: Cellular Originmentioning
confidence: 99%
“…5-4% of blood mononuclear leucocytes [3]. Progenitors of tissue DC are also present in human cord blood and peripheral blood [4,5] and their numbers are expanded following stimulation with the cytokines tumour necrosis factor-alpha (TNF-a) and granulocytemacrophage colony-stimulating factor (GM-CSF) [5][6][7].…”
Section: Introductionmentioning
confidence: 99%