Identification of Highly Specific and Cross‐Reactive Antigens of <i>Leishmania</i> Species by Antibodies from <i>Leishmania (Leishmania) chagasi</i> Naturally Infected Dogs

Vale, Andre M.; Fujiwara, Ricardo Toshio; Neto, Amato; Miret, Jorge; Álvarez, D.; Silva, J. C. F. Da; Campos-Neto, Antônio; Reed, Steven G.; Mayrink, Wilson; Nascimento, Evaldo

doi:10.1111/j.1863-2378.2008.01183.x

Cited by 17 publications

(12 citation statements)

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“…Leishmania chagasi ) from Brazil have been shown to respond to antigen from L. major -like antigen by ELISA [24] and also to L. braziliensis antigen [25]. Furthermore, sera from Brazilian L. infantum ( chagasi )-infected dogs was reactive by western immunoblotting with several antigens from whole promastigote antigens of Leishmania guyanensis , Leishmania amazonensis and L. braziliensis [26]. Therefore, cross-reactivity amongst the Old World species of L. major , L. tropica and L. infantum as found in dogs in the current study is not surprising, and it is important to note that ELISA has not been found distinctive for the infecting Leishmania species with regard to the OD level of reactivity in this study.…”

Section: Discussionmentioning

confidence: 99%

Canine leishmaniosis caused by Leishmania major and Leishmania tropica: comparative findings and serology

et al. 2017

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BackgroundInfection and clinical disease associated with Leishmania major and Leishmania tropica, two common agents of human cutaneous leishmaniosis, have rarely been reported in dogs. This study describes dogs infected with these Leishmania spp. prevalent in the Middle East and North Africa, and compares the serological response of dogs infected with Leishmania infantum, L. major or L. tropica to whole promastigote antigen enzyme-linked immunosorbent assay (ELISA) of each species and to rK39 dipstick.Results Leishmania major infection in a 5-month-old male dog was associated with alopecic and ulcerative periocular and limb skin lesions which responded to allopurinol treatment. Infection was detected by skin and blood polymerase chain reaction (PCR) and confirmed by DNA sequencing but the dog was seronegative. Leishmania tropica infection was detected in a 3-month-old female dog co-infected with Babesia vogeli and Anaplasma platys and with no skin lesions. PCR and DNA sequencing of the blood and parasite culture were positive for L. tropica. Sera from 11 dogs infected with L. infantum, L. major or L. tropica were reactive with all three Leishmania spp. antigens except for sera from a dog with L. major infection. No significant differences were found between reactivity of dog sera to the antigen of the infecting species, or to the other Leishmania spp. antigens. Sera from dogs infected with L. infantum and L. tropica were positive with the rK39 antigen kit, while dogs with L. major infection were seronegative.ConclusionsSkin lesions in L. major infected dogs from this study and previous reports (n = 2) were ulcerative and located on the muzzle, feet and foot pads and not associated with generalized lymphadenomegaly and splenomegaly. In previous L. tropica infections, skin lesions were proliferative mucocutaneous in young dogs (n = 2), or associated with widespread dermatitis, lymphadenomegaly and splenomegaly in older dogs with similarity to L. infantum infection (n = 2). This study suggests that ELISA serology with whole promastigote antigen is not distinctive between L. infantum, L. major and L. tropica canine infections and that some L. major infections are not seropositive. PCR with DNA sequencing should be used to discriminate between canine infections with these three species.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2050-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Canine leishmaniosis caused by Leishmania major and Leishmania tropica: comparative findings and serology

et al. 2017

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“…Despite testing positive serologically, three dogs from the asymptomatic group, including a three-to-four-month-old puppy, tested negatively for all of the administered tests. Possible explanations include false seropositivity 40 , cross-reactivity with other diseases 41 or the presence of a pre-patent infection 42,43 . In the case of the puppy, the positive serology might also be attributable to the presence of maternal antibodies 44 .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of parasitological examination, kDNA polymerase chain reaction and rK39-based immunochromatography for the diagnosis of visceral leishmaniasis in seropositive dogs from the screening-culling program in Brazil

Regina-Silva

Fortes-Dias

Michalsky

et al. 2014

Rev. Soc. Bras. Med. Trop.

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Introduction: Dogs play a primary role in the zoonotic cycle of visceral leishmaniasis (VL). Therefore, the accurate diagnosis of infected dogs, primarily asymptomatic dogs, is crucial to the effi ciency of VL control programs. Methods: We investigated the agreement of four diagnostic tests for canine visceral leishmaniasis (CVL): parasite detection, either after myeloculture or by direct microscopic examination of tissue imprints; kinetoplast-deoxyribonucleic acid-polymerase chain reaction (kDNA-PCR); and an immunochromatographic test (ICT). An enzyme-linked immunosorbent assay (ELISA) and an indirect immunofl uorescence test (IFAT), both of which were adopted as part of the screening-culling program in Brazil, were used as reference tests. Our sample set consisted of 44 seropositive dogs, 25 of which were clinically asymptomatic and 19 were symptomatic for CVL according to ELISA-IFAT. Results: The highest and lowest test co-positivities were observed for ICT (77.3%) and myeloculture (58.1%), respectively. When analyzed together, the overall percentage of co-positive tests was signifi cantly higher for the symptomatic group compared to the asymptomatic group. However, only ICT was signifi cantly different based on the results of a separate analysis per test for each group of dogs. The majority (93.8%) of animals exhibited at least one positive test result, with an average of 2.66 positive tests per dog. Half of the symptomatic dogs tested positive for all four tests administered. Conclusions:The variability between test results reinforces the need for more effi cient and reliable methods to accurately diagnose canine VL, particularly in asymptomatic animals.

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“…The presence of antibodies against T. gondii, N. (VALE et al, 2009), and seropositive results only for the genus (Leishmania spp.) were considered in this study.…”

Section: Resultsmentioning

confidence: 99%

Presence of anti-Toxoplasma gondii, -Neospora caninum, -Leishmania spp. and -Ehrlichia canis antibodies in free-ranging maned wolves (Chrysocyon brachyurus) in the northeastern region of the state of São Paulo, Brazil

Oliveira

Silveira-Mattos

Mattos

et al. 2016

Braz. J. Vet. Res. Anim. Sci.

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O lobo-guará (Chrysocyon brachyurus) habita o ecossistema de Cerrado e é considerado o maior canídeo da América do Sul e uma espécie ameaçada de extinção pela "International Union for Conservation of Nature" (IUNC). O objetivo desse estudo foi investigar a presença de anticorpos anti-Toxoplasma gondii, -Neospora caninum, -Leishmania spp. e -Ehrlichia canis em lobos-guará da região nordeste do estado de São Paulo, Brasil. Das 17 amostras de soro testadas por meio da reação de imunofluorescência indireta (RIFI), 88,2% (15/17), 17,6% (3/17) e 52,9% (9/17) apresentaram anticorpos anti-T. gondii, -Leishmania spp. e -E. canis, respectivamente. Todos os animais testados foram soronegativos para N. caninum. Esses resultados indicam a exposição dos lobos-guará dessa região aos agentes pesquisados. A presença de um complexo industrial, agricultura extensiva e fragmentação de habitat na região nordeste do estado de São Paulo, favorece a proximidade desses animais silvestres a ambientes urbanos o que pode contribuir para a transmissão de doenças entre os animais silvestres, domésticos e o homem.

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Identification of Highly Specific and Cross‐Reactive Antigens of Leishmania Species by Antibodies from Leishmania (Leishmania) chagasi Naturally Infected Dogs

Cited by 17 publications

References 48 publications

Canine leishmaniosis caused by Leishmania major and Leishmania tropica: comparative findings and serology

Canine leishmaniosis caused by Leishmania major and Leishmania tropica: comparative findings and serology

Evaluation of parasitological examination, kDNA polymerase chain reaction and rK39-based immunochromatography for the diagnosis of visceral leishmaniasis in seropositive dogs from the screening-culling program in Brazil

Presence of anti-Toxoplasma gondii, -Neospora caninum, -Leishmania spp. and -Ehrlichia canis antibodies in free-ranging maned wolves (Chrysocyon brachyurus) in the northeastern region of the state of São Paulo, Brazil

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