Identification of hrpL up-regulated genes of Dickeya dadantii

Shi, Xiangyang; Cooksey, Donald A.

doi:10.1007/s10658-008-9397-8

Cited by 2 publications

(4 citation statements)

References 31 publications

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“…However, the severity of soft-rot symptoms was reduced profoundly in the hrpL mutant when inoculated onto the tobacco leaf surface, mimicking a natural infection process. This finding was consistent with previous reports of the reduced pathogenicity of the HrpL mutant of D. dadantii (Shi and Cooksey, 2009) and the HrcC mutant of P. carotovorum (Rantakari et al, 2001) on susceptible host plants. Thus, HrpL may regulate other genes involved in the development of soft-rot symptoms.…”

Section: Discussionsupporting

confidence: 93%

“…The functional roles of a few hrp genes of plant pathogenic Pectobacterium-related bacteria have also been identified. Mutants of the hrpL-regulated genes in D. dadantii evidenced reduced pathogenicity (Shi and Cooksey, 2009). Mutation of the HrpLregulated hrcC gene in P. carotovorum did not impair pathogenicity, even though early growth on Arabidopsis was reduced (Rantakari et al, 2001).…”

Section: Introductionmentioning

confidence: 91%

“…Recently, five hrpL up-regulated genes, hrpA, hrpK, dspE, yijC and yecF of Dickeya dadantii (Erwinia chrysanthemi), were identified by a green fluorescence (GFP)-based E. coli promoter-probe system (Shi and Cooksey, 2009). The functional roles of a few hrp genes of plant pathogenic Pectobacterium-related bacteria have also been identified.…”

Section: Introductionmentioning

confidence: 99%

“…In an effort to identify the genes regulated by HrpL in P. carotovora 35, we employed the GFP-based strategy used for D. dadantti (Shi and Cooksey, 2009), which identifies the promoters required for pathogenesis (Shi and Cooksey, 2009;Yang et al, 2004). We selected genes that were expressed only in the presence of HrpL.…”

Section: Introductionmentioning

confidence: 99%

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Alternative Sigma Factor HrpL of Pectobacterium carotovorum 35 is Important for the Development of Soft-rot Symptoms

Nam¹,

Park²,

Kang³

et al. 2011

Research in Plant Disease

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A bacterial artificial chromosome library of Pectobacterium carotovorum 35 was constructed to characterize the genome and to sequence its hrp region. The hrp cluster of P. carotovorum 35 consisted of 26 open reading frames in five operons. A promoter-based green fluorescent protein technology was used to identify the genes regulated by the alternative sigma factor, HrpL, in P. carotovorum 35. The majority of the selected clones contained the hrpJ operon promoter sequence, which harbors a hrp box, but no putative hrp boxes were detected within the promoter sequences of two other hrpL-regulated genes encoding for pectate lyase and large repetitive protein. Although the promoters of five other hrp operons also contained hrp boxes, their expression was not HrpL-dependent in the promoter-based selection in E. coli. However, transcriptional analysis showed that expression from all operons harboring hrp boxes, except for the hrpN operon, was reduced significantly in the hrpL mutant. The severity of soft-rot symptoms when the hrpL mutant was applied to the surface of tobacco leaves, mimicking natural infection, was greatly attenuated. These results indicate that the hrpL gene of P. carotovorum 35 may be involved in the development of soft-rot symptoms.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%