Identification of hyperthermophilic D-allulose 3-epimerase from Thermotoga sp. and its application as a high-performance biocatalyst for D-allulose synthesis

Shen, Ji-Dong; Xu, Bao-Ping; Yu, Te-Li; Fei, Yong-Xiang; Cai, Xue; Huang, Liang-Gang; Jin, Li-Qun; Liu, Zhi-Qiang; Zheng, Yu-Guo

doi:10.1007/s00449-024-02989-3

Bioprocess Biosyst Eng

2024

DOI: 10.1007/s00449-024-02989-3

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Identification of hyperthermophilic D-allulose 3-epimerase from Thermotoga sp. and its application as a high-performance biocatalyst for D-allulose synthesis

Ji-Dong Shen,

Bao-Ping Xu,

Te-Li Yu

et al.

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Cited by 2 publications

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Biosynthesis of nonnutritive monosaccharide d‐allulose by metabolically engineered Escherichia coli from nutritive disaccharide sucrose

Zheng,

Chen,

Zheng

et al. 2024

Biotech & Bioengineering

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Sucrose is a commonly utilized nutritive sweetener in food and beverages due to its abundance in nature and low production costs. However, excessive intake of sucrose increases the risk of metabolic disorders, including diabetes and obesity. Therefore, there is a growing demand for the development of nonnutritive sweeteners with almost no calories. d‐Allulose is an ultra‐low‐calorie, rare six‐carbon monosaccharide with high sweetness, making it an ideal alternative to sucrose. In this study, we developed a cell factory for d‐allulose production from sucrose using Escherichia coli JM109 (DE3) as a chassis host. The genes cscA, cscB, cscK, alsE, and a6PP were co‐expressed for the construction of the synthesis pathway. Then, the introduction of ptsG‐F and knockout of ptsG, fruA, ptsI, and ptsH to reprogram sugar transport pathways resulted in an improvement in substrate utilization. Next, the carbon fluxes of the Embden‐Meyerhof‐Parnas and the pentose phosphate pathways were regulated by the inactivation of pfkA and zwf, achieving an increase in d‐allulose titer and yield of 154.2% and 161.1%, respectively. Finally, scaled‐up fermentation was performed in a 5 L fermenter. The titer of d‐allulose reached 11.15 g/L, with a yield of 0.208 g/g on sucrose.

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Biosynthesis of nonnutritive monosaccharide d‐allulose by metabolically engineered Escherichia coli from nutritive disaccharide sucrose

Zheng,

Chen,

Zheng

et al. 2024

Biotech & Bioengineering

View full text Add to dashboard Cite

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Engineering Escherichia coli for D-allulose biosynthesis from glycerol

Guo,

Dong,

Luo

et al. 2024

Journal of Biotechnology

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Identification of hyperthermophilic D-allulose 3-epimerase from Thermotoga sp. and its application as a high-performance biocatalyst for D-allulose synthesis

Cited by 2 publications

References 25 publications

Biosynthesis of nonnutritive monosaccharide d‐allulose by metabolically engineered Escherichia coli from nutritive disaccharide sucrose

Biosynthesis of nonnutritive monosaccharide d‐allulose by metabolically engineered Escherichia coli from nutritive disaccharide sucrose

Engineering Escherichia coli for D-allulose biosynthesis from glycerol

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