Identification of
            <i>Bacteroides thetaiotaomicron</i>
            on the Basis of an Unexpected Specific Amplicon of Universal 16S Ribosomal DNA PCR

Teng, Lee‐Jene; Hsueh, Po-Ren; Huang, Yu-Hsuan; Tsai, Jui‐Chang

doi:10.1128/jcm.42.4.1727-1730.2004

Cited by 27 publications

(22 citation statements)

References 18 publications

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“…Pathogenic bacteria can be detected or diagnosed by using a variety of test methods, include growth on selective media followed by biochemical test, in addition to nucleic acid detection by polymerase chain reaction (PCR). The present study used PCR technique targeting 16S rRNA to detect bacterial isolates from infected fish through universal primers (RW01 and DG74) which has been reported as a sensitive screening method for detecting bacterial communities (Greisen et al, 1994;Teng et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Isolation and molecular characterization of some bacterial pathogens in El-Serw fish farm, Egypt

El‐Barbary¹,

Hal²

2016

Egypt. J. of Aquatic Biolo. and Fish.

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The current study was carried out to isolate and identify some of bacteria infecting fish of El-Serw fish farm during the harvest season from December to March 2014-2015. Fish samples (155) were collected from different ponds of farm; 65 Nile tilapia (Oreochromis niloticus), 55 catfish (Clarias gariepinus) and 35 mullet (Liza ramada) were used to isolate bacteria in fish organs (gills, liver and kidney) using different selective media, from the examined fish, bacteria belonging to Pseudomonas, Aeromonas and Enterobacteriaceae were isolated. The group most frequently isolated from fish was Aeromonas, the highest number of putative Aeomonas isolates were obtained from O. niloticus 19 (29%), followed C. gariepinus 12 (21.8%) and L. ramada 5 (14.2%). Whereas putative Pseudomonas sp. showed fewer prevalence 9 (13.8%), 8 (14.5%) and 4 (11.4%) from O. niloticus, C. gariepinus and L. ramada, respectively, while only two colonies were recovered from Yersinia Agar Base. Seven isolates were selected and identified by sequencing of 16S rRNA. The analysis of sequence of 16S rRNA gene using universal primers (RW01 and DG74) resulted in the identification of three bacterial isolates of Pseudomonas fluorescens, one of Pseudomonas putida, two of Aeromonas hydrophila and one of Klebsiella oxytoca. The antibiogram test of these isolates revealed their sensitivity to ciprofloxacin, norfloxacin, and gentamycin.This study concluded that the performance 16S rRNA assay has the prospective to create an important contribution to infection management providing rapid of identification the bacterial pathogens in fish.

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Section: Discussionmentioning

confidence: 99%

Isolation and molecular characterization of some bacterial pathogens in El-Serw fish farm, Egypt

El‐Barbary¹,

Hal²

2016

Egypt. J. of Aquatic Biolo. and Fish.

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“…Total genomic DNA was purified from each microbiota, and PCR using primers specific for B. thetaiotaomicron was performed as described previously (35).…”

Section: Methodsmentioning

confidence: 99%

Human Microbiota-Secreted Factors Inhibit Shiga Toxin Synthesis by Enterohemorrhagic Escherichia coli O157:H7

et al. 2009

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Escherichia coli O157:H7 is a food-borne pathogen causing hemorrhagic colitis and hemolytic-uremic syndrome, especially in children. The main virulence factor responsible for the more serious disease is the Shiga toxin 2 (Stx2), which is released in the gut after oral ingestion of the organism. Although it is accepted that the amount of Stx2 produced by E. coli O157:H7 in the gut is critical for the development of disease, the eukaryotic or prokaryotic gut factors that modulate Stx2 synthesis are largely unknown. In this study, we examined the influence of prokaryotic molecules released by a complex human microbiota on Stx2 synthesis by E. coli O157:H7. Stx2 synthesis was assessed after growth of E. coli O157:H7 in cecal contents of gnotobiotic rats colonized with human microbiota or in conditioned medium having supported the growth of complex human microbiota. Extracellular prokaryotic molecules produced by the commensal microbiota repress stx 2 mRNA expression and Stx2 production by inhibiting the spontaneous and induced lytic cycle mediated by RecA. These molecules, with a molecular mass of below 3 kDa, are produced in part by Bacteroides thetaiotaomicron, a predominant species of the normal human intestinal microbiota. The microbiota-induced stx 2 repression is independent of the known quorum-sensing pathways described in E. coli O157:H7 involving SdiA, QseA, QseC, or autoinducer 3. Our findings demonstrate for the first time the regulatory activity of a soluble factor produced by the complex human digestive microbiota on a bacterial virulence factor in a physiologically relevant context.

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“…The present study used PCR technique targeting 16S rRNA for detection of bacterial isolates from infected fish through universal primers (RW01 and DG74), that was reported to be a sensitive screening technique to detect the bacterial communities' [38,56].…”

Section: Histopathological Examinationmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Some Pseudomonas sp. Associated with Burkholderia cepacia Isolated from Various Infected Fishes

El‐Barbary¹,

Hal²

2017

J Aquac Res Development

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Identification of Bacteroides thetaiotaomicron on the Basis of an Unexpected Specific Amplicon of Universal 16S Ribosomal DNA PCR

Cited by 27 publications

References 18 publications

Isolation and molecular characterization of some bacterial pathogens in El-Serw fish farm, Egypt

Isolation and molecular characterization of some bacterial pathogens in El-Serw fish farm, Egypt

Human Microbiota-Secreted Factors Inhibit Shiga Toxin Synthesis by Enterohemorrhagic Escherichia coli O157:H7

Phenotypic and Genotypic Characterization of Some Pseudomonas sp. Associated with Burkholderia cepacia Isolated from Various Infected Fishes

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