Identification of <i>C</i>‐glycoside Flavonoids as Potential Mutagenic Compounds in Kava

Jhoo, Jin‐Woo; Ang, C. Y. W.; Heinze, Thomas M.; Deck, J. F.; Schnackenberg, Laura K.; Beger, Richard D.; Dragull, Klaus; Tang, Chung-Shih

doi:10.1111/j.1750-3841.2007.00278.x

Cited by 18 publications

(10 citation statements)

References 22 publications

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“…Several investigators demonstrated the cytotoxic/mutagenic potential assessment of some flavonoids present in C. oxyacantha or other plant genus. Jhoo et al (2007) identified C-glycoside flavonoids 2-Orhamnosylvitexin and schaftoside as potential mutagenic compounds in Kava (Piper methysticum) by the umu test, a sensitive test for point mutations. Snijman et al (2007) evaluating the antimutagenic activity of the major flavonoids of rooibos (Aspalathus linearis), by using the Ames test, observed that flavonoid-mutagen interactions ranged from antimutagenic, comutagenic, and promutagenic to mutagenic, while vitexin, isovitexin, and hyperoside exhibited only antimutagenic effects.…”

Section: Discussionmentioning

confidence: 99%

Fruit extract of the medicinal plant Crataegus oxyacantha exerts genotoxic and mutagenic effects in cultured cells

Quadros

Mazzeo

Marin-Morales

et al. 2017

Journal of Toxicology and Environmental Health, Part A

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Crataegus oxyacantha, a plant of the Rosaceae family also known "English hawthorn, haw, maybush, or whitethorn," has long been used for medicinal purposes such as digestive disorders, hyperlipidemia, dyspnea, inducing diuresis, and preventing kidney stones. However, the predominant use of this plant has been to treat cardiovascular disorders. Due to a lack of studies on the genotoxicity of C. oxyacantha, this investigation was undertaken to determine whether its fruit extract exerts cytotoxic, genotoxic, or clastogenic/aneugenic effects in leukocytes and HepG2 (liver hepatocellular carcinoma) cultured human cells, or mutagenic effects in TA100 and TA98 strains of Salmonella typhimurium bacterium. Genotoxicity analysis showed that the extract produced no marked genotoxic effects at concentrations of 2.5 or 5 µg/ml in either cell type; however, at concentrations of 10 µg/ml or higher significant DNA damage was detected. The micronucleus test also demonstrated that concentrations of 10 µg/ml or higher produced clastogenic/aneugenic responses. In the Ames test, the extract induced mutagenic effects in TA98 strain of S. typhimurium with metabolic activation at all tested concentrations (2.5 to 500 µg/ml). Data indicate that, under certain experimental conditions, the fruit extract of C. oxyacantha exerts genotoxic and clastogenic/aneugenic effects in cultured human cells, and with metabolism mutagenicity occurs in bacteria cells.

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Section: Discussionmentioning

confidence: 99%

Fruit extract of the medicinal plant Crataegus oxyacantha exerts genotoxic and mutagenic effects in cultured cells

Quadros

Mazzeo

Marin-Morales

et al. 2017

Journal of Toxicology and Environmental Health, Part A

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“…Similarly, G. biloba extract or supplements containing quercetin or kaempferol may inhibits intestinal or hepatic glucuronidation of mycophenolic acid [131]. Kava-kava ( Piper methysticum ) is well know for its hepato-toxicity, and flavonoid C-glycosides isolated from Kava extract show mutagenic effects [132]. These results indicate that some dietary flavonoids may have the potential to negatively interact with clinical drugs.…”

Section: Potential Toxicities and Quality Control Of Dietary Supplementsmentioning

confidence: 99%

Flavonoids and age-related disease: Risk, benefits and critical windows

2010

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Plant derived products are consumed by a large percentage of the population to prevent, delay and ameliorate disease burden; however, relatively little is known about the efficacy, safety and underlying mechanisms of these traditional health products, especially when taken in concert with pharmaceutical agents. The flavonoids are a group of plant metabolites that are common in the diet and appear to provide some health benefits. While flavonoids are primarily derived from soy, many are found in fruits, nuts and more exotic sources, e.g., kudzu. Perhaps the strongest evidence for the benefits of flavonoids in diseases of aging relates to their effect on components of the metabolic syndrome. Flavonoids from soy, grape seed, kudzu and other sources all lower arterial pressure in hypertensive animal models and in a limited number of tests in humans. They also decrease the plasma concentration of lipids and buffer plasma glucose. The underlying mechanisms appear to include antioxidant actions, central nervous system effects, gut transport alterations, fatty acid sequestration and processing, PPAR activation and increases in insulin sensitivity. In animal models of disease, dietary flavonoids also demonstrate a protective effect against cognitive decline, cancer and metabolic disease. However, research also indicates that the flavonoids can be detrimental in some settings and, therefore, are not universally safe. Thus, as the population ages, it is important to determine the impact of these agents on prevention/attenuation of disease, including optimal exposure (intake, timing/duration) and potential contraindications.

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“…Jhoo et al (2007) testaram a mutagenicidade de 6 kavalactonas isoladas da raiz de kava-kava no umu teste, em Salmonella typhimurium TA1535/ pSK 1002. Nenhuma das kavalactonas apresentou mutagenicidade neste teste, que detecta mutações de ponto.…”

Section: Resultado E Discussãounclassified

Avaliação da mutagenicidade de Piper methysticum L. f. no sistema methG1 em Aspergillus nidulans

Palioto

Rocha

2013

Rev. bras. plantas med.

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Recebido para publicação em 05/11/2010 Aceito para publicação em 12/11/2012 RESUMO: O extrato seco da raiz de Piper methysticum L. f. Forster (PIPERACEAE), a kava-kava, é usado no tratamento de diversos problemas envolvendo ansiedade como um dos sintomas. Por não causar dependência, sedação e ter ação ansiolítica, muitas pessoas têm recorrido a kava-kava para auxiliá-las no emagrecimento. Isto pode levar ao consumo indiscriminado da planta e acarretar riscos, pois todo medicamento fitoterápico deve respeitar limites de doses. Um risco na utilização de plantas medicinais é a toxicidade e, dentro deste, a mutagenicidade. Como a mutagenicidade está relacionada com a carcinogenicidade tornase importante testar este potencial na kava-kava. Assim, o objetivo deste trabalho foi avaliar o potencial mutagênico do extrato seco da raiz de P. methysticum no sistema methG1 em Aspergillus nidulans. A linhagem utilizada foi a biA1methG1, auxotrófica para biotina e metionina. Conídios dormentes de colônias crescidas por cinco dias foram tratados com soluções da kavakava nas concentrações de 0,35 mg mL -1 e 3,5 mg mL -1 , e depois de 24h, semeados em meio seletivo contendo metionina, para análise dos sobreviventes, e sem metionina, para a análise dos mutantes. Os números de sobreviventes e mutantes dos tratamentos foram comparados aos do controle. Os resultados indicaram que o extrato da raiz da kava-kava é mutagênica, pois a freqüência de mutação dos tratamentos foi maior que da mutação espontânea, porém não ocorrendo diferença significativa entre as doses. Palavras-chave:Piper methysticum L. f., kava-kava, mutagenicidade, sistema methG1, Aspergillus nidulans ABSTRACT: Mutagenic evaluation of Piper methysticum L. f. in Aspergillus nidulans methG1 system. The dry root extract of Piper methysticum L. f. Forster (Piperaceae), kavakava, is used as to treat several health problems involving anxiety symptoms. As it causes no addiction, it can be applied as a sedative and anxiolytic. Many people have been relying on kava-kava as an auxiliary treatment. This can lead to an indiscriminate plant consumption and lead to risks, because all phytotherapic medications must observe dosage limits. One risk in the folk medicinal plant use is toxicity, and within it, mutagenicity. As mutagenicity is closely related to carcinogenicity, it is important to test the kava-kava mutagenicity potential. Thus, the purpose of this work was to test the mutagenicity of the dry root extract of P. methysticum in the methG1 system of Aspergillus nidulans. The bia1methG1 lineage, which is auxotrophic for biotine and methione, was used. Conidia from five-day-old colonies were collected and treated with kava-kava solutions at 0.35 mg mL -1 and 3.5 mg mL -1 concentrations and, after 24h, they were planted in selective growth medium with and without methione, in order to analyze the survivors and mutants, respectively. The number of survivors and mutants analyzed under effect of the treatments was compared with the control. The results indicated that the kava-kava d...

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Identification of C‐glycoside Flavonoids as Potential Mutagenic Compounds in Kava

Cited by 18 publications

References 22 publications

Fruit extract of the medicinal plant Crataegus oxyacantha exerts genotoxic and mutagenic effects in cultured cells

Fruit extract of the medicinal plant Crataegus oxyacantha exerts genotoxic and mutagenic effects in cultured cells

Flavonoids and age-related disease: Risk, benefits and critical windows

Avaliação da mutagenicidade de Piper methysticum L. f. no sistema methG1 em Aspergillus nidulans

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