Identification of
            <i>Enterococcus</i>
            spp. with a Biochemical Key

Manero, Albert; Blanch, Anicet R.

doi:10.1128/aem.65.10.4425-4430.1999

Cited by 255 publications

(142 citation statements)

References 62 publications

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“…Sugar fermentation pattern of strain QU 2 was tested by the API 50 CHL system (bioMérieux, Marcy l'Etoile, France). The obtained pattern was compared with those of reference strains, described by Manero and Blanch (1999).…”

Section: Identification Of Strain Qumentioning

confidence: 99%

Identification and production of a bacteriocin from Enterococcus mundtii QU 2 isolated from soybean

et al. 2005

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Aims: Identification of the bacteriocin produced by Enterococcus mundtii QU 2 newly isolated from soybean and fermentative production of the bacteriocin. Methods and Results: The bacteriocin produced by Ent. mundtii QU 2 inhibited the growth of various indicator strains, including Enterococcus, Lactobacillus, Leuconostoc, Pediococcus and Listeria. The bacteriocin activity was stable at wide pH range and against heat treatment, but completely abolished by proteolytic enzymes. The bacteriocin was purified from the culture supernatant by the three-step chromatographic procedure. Mass spectrometry, amino acid sequencing and DNA sequencing revealed that the bacteriocin was similar to class IIa bacteriocins produced by other Ent. mundtii strains. The bacteriocin production decreased in the absence of glucose, nitrogen sources, or Tween 80 in MRS medium. Additionally, it was strongly suppressed by addition of Ca 2+ (CaCO 3 or CaCl 2 ). In pH-controlled fermentations, the highest bacteriocin production was achieved at pH 6AE0, whereas the highest cell growth was obtained at pH 7AE0. Conclusions: Ent. mundtii QU 2 produced a class IIa bacteriocin. Some growth factors (e.g. Ca 2+ and pH) influenced the bacteriocin production. Significance and Impact of the Study: A new soybean isolate, Ent. mundtii QU 2 was found to be a class IIa bacteriocin producer. Factors influencing the bacteriocin production described herein are valuable for applications of the bacteriocins from Ent. mundtii strains.

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Section: Identification Of Strain Qumentioning

confidence: 99%

Identification and production of a bacteriocin from Enterococcus mundtii QU 2 isolated from soybean

et al. 2005

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“…In addition, typical metabolic properties of Ent. durans (Manero and Blanch 1999) such as ability to utilize arbutin but not l-arabinose, mannitol, sorbitol, and d-tagotose were also observed in strains QU 49 in the API 50CHL-based carbohydrate utilization assay, and further supported that strain QU 49 belongs to Ent. durans.…”

Section: Isolation and Taxonomic Analysis Of Strain Qu 49mentioning

confidence: 55%

Description of durancin TW-49M, a novel enterocin B-homologous bacteriocin in carrot-isolatedEnterococcus duransQU 49

Zendo

Nakayama

et al. 2008

Journal of Applied Microbiology

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Aims: To characterize the novel bacteriocin produced by Enterococcus durans. Methods and Results: Enterococcus durans QU 49 was isolated from carrot and expressed bactericidal activity over 20–43°C. Bacteriocins were purified to homogeneity using the three‐step purification method, one of which, termed durancin TW‐49M, was an enterocin B‐homologous peptide with most identical residues occurring in the N‐terminus. Durancin TW‐49M was more tolerant in acidic than in alkali. DNA sequencing analysis revealed durancin TW‐49M was translated as a prepeptide of the double‐glycine type. Durancin TW‐49M and enterocin B expressed similar antimicrobial spectra, in which no significant variation due to the diversity in their C‐termini was observed. Conclusions: Durancin TW‐49M, a novel nonpediocin‐like class II bacteriocin, was characterized to the amino acid and genetic levels. The diverse C‐terminal parts of durancin TW‐49M and enterocin B were hardly to be suggested as the place determining the target cell specificity. Significance and Impact of the Study: This is the first and comprehensive study of a novel bacteriocin produced by Ent. durans. The high homology at the N‐terminal halves between durancin TW‐49M and enterocin B makes them suitable to study the structure‐function relationship of bacteriocins and their immunity proteins.

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“…The CL method is based on the detection of photon emission produced by different probes, such as luminol and lucigenine, in response to oxygen metabolites generated by different cells [21][22][23][24][25][26][27][28][29][30][31]. In the presence of oxygen species, light production emitted by luminol depends on the formation of an unstable endoperoxide or dioxetane, which decomposes to an electronically excited product, which releases a photon as it falls to the basal state.…”

Section: Discussionmentioning

confidence: 99%

“…Identification of the different strains was achieved using the biochemical keys of Schlegel and Bouvet [26] and Manero and Blanch [27]. Isolates were tested for the production of yellow pigment, arginine di-hydrolase (ADH) (BioRad, France) production, tellurite 0.04% reduction, mobility and the production of acid from L-arabinose, lactose, mannitol, D-raffinose, and ribose (Sigma, France).…”

Section: Phenotypic Species Characterizationmentioning

confidence: 99%

Chemiluminescence of enterococci isolates from freshwater

André¹,

Metzger²,

Petey³

et al. 2005

FEMS Microbiology Letters

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All Enterococcus spp., isolated from environmental water samples (n=81), emitted a high chemiluminescence signal in the presence of luminol (10(-2) M). Kinetic studies of chemiluminescence show a close correlation between chemiluminescence and growth curves during the exponential phase, with a maximum chemiluminescence reached just before bacterial growth entered in the stationary phase. On the other hand, genera closely related to Enterococcus such as Streptococcus or Lactococcus produced a very weak chemiluminescent signal. Chemiluminescence of enterococci could therefore offer a rapid test, in aiding the identification of the genus Enterococcus and in the survey of the microbiological quality of water supplies.

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Identification of Enterococcus spp. with a Biochemical Key

Cited by 255 publications

References 62 publications

Identification and production of a bacteriocin from Enterococcus mundtii QU 2 isolated from soybean

Identification and production of a bacteriocin from Enterococcus mundtii QU 2 isolated from soybean

Description of durancin TW-49M, a novel enterocin B-homologous bacteriocin in carrot-isolatedEnterococcus duransQU 49

Chemiluminescence of enterococci isolates from freshwater

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