Identification of <i>Escherichia coli</i> 01 57:H7‐ strains from pigs with postweaning diarrhoea and amplification of their virulence marker genes by PCR

Osek, Jacek

doi:10.1136/vr.150.22.689

Cited by 9 publications

(11 citation statements)

References 44 publications

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“…PCR analyses. All PCR tests were performed before and their results had been published previously (Osek, 1999(Osek, , 2002Osek et al, 2000Osek et al, , 2003. Briefly, E. coli strains were grown overnight at 37°C on LB agar.…”

Section: Methodsmentioning

confidence: 99%

“…One strain was obtained from one animal that represented one ca�le farm. The strains of porcine origin (n = 38) were isolated from weaned pigs (four to six weeks old) with diarrhea from eight geographically separated pig farms in the west part of Poland as described previously (Osek, 2002).…”

Section: Methodsmentioning

confidence: 99%

“…The resulting supernatant was used as a template for PCR. The following genes were amplified: r�O157 (E. coli O157), fliC (H7), stx1 and stx2 (Shiga toxin 1 and 2, respectively), stx2e (Shiga toxin 2e variant), ehly (enterohemolysin), eaeA (intimin), and lpf O113 (long polar fimbriae) using the PCR primers and amplification conditions as described previously (Osek, 1999(Osek, , 2002Osek et al, 2000Osek et al, , 2003.…”

Section: Methodsmentioning

confidence: 99%

“…a Phenotypic properties were tested as described in Material and Methods. The respective genes were analysed previously by PCR (Osek, 2002;Osek et al, 2003) b Additional 20 strains possessed genes encoding Stx2e toxin variant as determined by PCR (Osek, 1999) It was demonstrated that Shiga toxins 1 and 2 were produced by E. coli O157 strains isolated from all 3 sources (humans, ca�le, pigs) (Table 1). It was found that all human and bovine isolates could be classified to the STEC group and were able to produce either one toxin (Stx1 or Stx2) alone or both.…”

Section: Characteristics Of E Coli Strains Testedmentioning

confidence: 99%

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Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Osek¹

2004

Vet. Med. - Czech

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A total of 90 Escherichia coli O157 isolates recovered from humans, cattle, and pigs, were examined for the presence of the H7 antigen, ability to produce Shiga toxins and enterohemolysin as well as for antimicrobial resistance and biochemical properties. Fourteen of the human strains (n = 23) and 21 of the bovine isolates (n = 29) were of the O157:H7 serotype as determined by agglutination and PCR methods. All E. coli O157 of porcine origin (n = 38) were H-negative. Based on the ability to produce Shiga toxins (Stxs), all human and cattle isolates and 11 strains recovered from swine were identified as Shiga toxin-producing E. coli (STEC). Among STEC, most human strains (18 isolates) were Stx1- and Stx2-positive whereas cattle strains were mostly Stx2-positive. Eleven porcine STEC produced either Stx1 (7 isolates) or Stx2 (4 strains) toxins; an additional 20 isolates recovered from these animals had the Stx2e toxin gene as previously determined by PCR. All human and cattle E. coli O157 produced enterohemolysin whereas only 4 strains recovered from pigs were ehly-positive. Moreover, the PCR identification of the lpf<sub>O113</sub> gene performed earlier revealed that this putative virulence marker was present in all porcine isolates, only in 5 strains of bovine origin but in none of E. coli O157 recovered from humans. All 90 E. coli O157 strains tested displayed 10 biochemical profiles that were different at least in one of the reaction tested. The most common atypical reaction observed among porcine O157 isolates was ability to ferment sorbitol (all strains) and production of β-glucuronidase (25 isolates). Moreover, none of the sorbitol-positive strains was able to produce indol. Four antimicrobial resistance profiles among 90 E. coli O157 strains tested were observed. Most of the isolates recovered from humans and all strains from cattle were resistant only to rifampicin whereas the porcine strains showed resistance to either 3 antimicrobials (4 isolates) or to 4 drugs tested (34 isolates). The phenotypic data shown in the present study, together with the previously published genotypic analyses of these strains, confirm earlier suggestions that the porcine E. coli O157 strains are mostly different from those of bovine and human O157 isolates and could therefore play less important role in human STEC O157 infections.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Characteristics Of E Coli Strains Testedmentioning

confidence: 99%

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Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Osek¹

2004

Vet. Med. - Czech

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“…Most of these isolates were recovered from cattle and from children with diarrhoea. Moreover, there is only one report describing the prevalence of E. coli O157 of pig origin (Osek, 2002) and there are few papers about such strains from other countries (Beutin et al, 1993;Chapman et al, 1997;Heuvelink et al, 1999;Nakazawa et al, 1999). In the present study, twelve Polish isolates (seven of bovine origin and five recovered from pigs, respectively) and two German strains (both isolated from cattle) were tested for STEC virulence marker genes and for their clonal relationship using PCR and PFGE methods, respectively.…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of Escherichia coli O157 strains isolated from cattle and pigs by the use of PCR and pulsed-field gel electrophoresis methods

Osek¹,

Gallien²

2002

Vet. Med. - Czech

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ABSTRACT:Fourteen Escherichia coli O157 strains isolated from cattle and pigs in Poland and in Germany were investigated, using PCR, for the genetic markers associated with Shiga toxin-producing E. coli (STEC). Only two strains, both of cattle origin, were positive for the fliC (H7) gene and could be classified as O157 : H7. Nine isolates had stx shiga toxin genes, either stx1 (1 strain), stx2 (4 isolates) or both (4 strains). e stx2-carrying samples were further subtyped by PCR for the stx2c, stx2d, and stx2e toxin variants. It was shown that all but one stx2-positive bacteria possessed the stx2c Shiga toxin gene type and one stx2 STEC isolate had the stx2d virulence factor subtype. e eaeA (intimin) gene was found in 9 strains (8 isolates from cattle and one strain from pigs); all of them harboured the genetic marker characteristic of the gamma intimin variant. e translocated intimin receptor (tir) gene was detected in 7 isolates tested and among them only one tir-positive strain was recovered from pigs. e ehly E. coli enterohemolysin gene was amplified in all but one strains obtained from cattle and only in one isolate of porcine origin. e genetic relatedness of the analysed E. coli O157 strains was examined by restriction fragment length polymorphism (RFLP) of chromosomal DNA digested with XbaI. Two distinct but related RFLP pattern clusters were observed: one with 9 strains (8 isolates of bovine origin and one strain obtained from pigs) and the other one comprises the remaining 5 E. coli isolates (4 of porcine origin and one strain recovered from cattle). e results suggest that pigs, besides cattle, may be a reservoir of E. coli O157 strains potentially pathogenic to humans. Moreover, epidemiologically unrelated isolates of the O157 serogroup, recovered from different animal species, showed a clonal relationship as demonstrated by the RFLP analysis.

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Characterization, estimation of virulence and drug resistance of diarrheagenic escherichia coli (DEC) isolated from Tibetan pigs

Cao

Shang

et al. 2023

Microbial Pathogenesis

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Identification of Escherichia coli 01 57:H7‐ strains from pigs with postweaning diarrhoea and amplification of their virulence marker genes by PCR

Cited by 9 publications

References 44 publications

Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Phenotypic and genotypic characterization of Escherichia coli O157 strains isolated from humans, cattle and pigs

Molecular analysis of Escherichia coli O157 strains isolated from cattle and pigs by the use of PCR and pulsed-field gel electrophoresis methods

Characterization, estimation of virulence and drug resistance of diarrheagenic escherichia coli (DEC) isolated from Tibetan pigs

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