Identification of <i>Gentiana rigescens</i> from different geographical origins based on HPLC and FTIR fingerprints

Zhao, Yanli; Tianjun, Yuan; Wu, Lihua; Zhang, Ji; Zuo, Zhiyan; Wang, Yuanzhong

doi:10.1039/d0ay00309c

Cited by 15 publications

(9 citation statements)

References 35 publications

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“…Comparative analyses related to phytochemical profiling of geographically distinct populations of numerous species revealed the need for more research regarding the impact of both genetic origin and a specific set of environmental conditions on the composition and content of secondary metabolites [ 24 ]. Several studies documented the chemical variability among natural populations in Gentiana species [ 20 , 25 , 26 , 27 , 28 , 29 , 30 ]. The study of high-altitude G. straminea showed significant interpopulation variability for several secondary metabolites, providing the basis for a geo-authentic production area for this traditional medicinal plant [ 28 ], and our results show that particular metabolites can even be absent in the chemical profile of some populations.…”

Section: Discussionmentioning

confidence: 99%

Gentiana asclepiadea L. from Two High Mountainous Habitats: Inter- and Intrapopulation Variability Based on Species’ Phytochemistry

Popović

Krstić-Milošević

Marković

et al. 2021

Plants

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Natural populations of Gentiana asclepiadea L., located at two mountainous sites, were HPLC-analyzed regarding the contents of six representative secondary metabolites. The contents of swertiamarin (SWM), gentiopicrin (GP), sweroside (SWZ), mangiferin (MGF), isoorientin (ISOOR), and isovitexin (ISOV) were determined in six populations (three per study site), and separately for aboveground and belowground plant parts. PCA showed a clear separation of four groups according to the contents of the analyzed secondary metabolites. Out of six analyzed compounds, five were present in all samples and only one (SWZ) was found in Golija populations (belowground parts) but not in Vlasina populations, and its presence can be indicative of the geolocation of populations. Clear separation of groups was mostly affected by the different contents of chemical compounds in plant parts (aboveground versus belowground) and by the differences related to population origin (higher content of SWM and GP in belowground parts of individuals from Vlasina populations and higher content of MGF and ISOOR of individuals from Golija populations). The results of this study contribute to the spatiochemical profiling of G. asclepiadea populations and a better understanding of inter- and intrapopulation variability of pharmacologically important compounds.

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Section: Discussionmentioning

confidence: 99%

Gentiana asclepiadea L. from Two High Mountainous Habitats: Inter- and Intrapopulation Variability Based on Species’ Phytochemistry

Popović

Krstić-Milošević

Marković

et al. 2021

Plants

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“…In recent years, many methods have been used to distinguish the geographical origin of licorice and other herbs [ 16 , 17 ], including high performance liquid chromatography [ 18 ], gas chromatography [ 19 ], nuclear magnetic resonance [ 20 ], electron nasal tongue [ 21 ], and near infrared spectroscopy [ 22 ], which come with high accuracy, but these methods are mainly analyzed by chemical composition. In recent years, stable isotopes have been widely used for origin tracing because of their excellent stability as well as the advantages of fast analysis, high precision, and significant effect.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, stable isotopes have been widely used for origin tracing because of their excellent stability as well as the advantages of fast analysis, high precision, and significant effect. By using the principle of natural fractionation effect of isotopes, samples of different origins show significant differences in the abundance of isotopes of elements ( 13 C/ 12 C, 15 N/ 14 N, 18 O/ 16 O) in natural substances due to differences in environment, climate, and soil. is information carries the environmental factors, reflects the environmental conditions of the organism and becomes a kind of "natural fingerprint", which can be used to distinguish substances of different geographical origins [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Chemistry Combining Elemental Profile, Stable Isotopic Ratios, and Chemometrics for Fine Classification of a Chinese Herb Licorice (Glycyrrhiza uralensis Fisch.) from 37 Producing Area

Lu¹,

Hai

Yan

et al. 2022

Journal of Analytical Methods in Chemistry

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A method based on elemental fingerprint, stable isotopic analysis and combined with chemometrics was proposed to trace the geographical origins of Licorice (Glycyrrhiza uralensis Fisch) from 37 producing areas. For elemental fingerprint, the levels of 15 elements, including Ca, Cu, Mg, Pb, Zn, Sr, Mn, Se, Cd, Fe, Na, Al, Cr, Co, and K, were analyzed by inductively coupled plasma atomic emission spectrometry (ICP-AES). Three stable isotopes, including δ13C, δ15N, and δ18O, were measured using an isotope-ratio mass spectrometer (IRMS). For fine classification, three multiclass strategies, including the traditional one-versus-rest (OVR) and one-versus-one (OVO) strategies and a new ensemble strategy (ES), were combined with two binary classifiers, partial least squares discriminant analysis (PLSDA) and least squares support vector machines (LS-SVM). As a result, ES-PLSDA and ES-LS-SVM achieved 0.929 and 0.921 classification accuracy of GUF samples from the 37 origins. The results show that element fingerprint and stable isotope combined with chemometrics is an effective method for GUF traceability and provides a new idea for the geographical traceability of Chinese herbal medicine.

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“…[19][20][21] Some studies have shown that high performance liquid chromatography (HPLC) combined with attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy can identify plants of different geographical origin. [22][23][24] Other studies have been carried out to identify the tissue parts of Panax plants through the integration of gas chromatography-mass spectrometry (GC-MS) and liquid chromatographymass spectrometry (LC-MS). 25 However, any data fusion strategy was not found to identify the parts and age of VG.…”

Section: Introductionmentioning

confidence: 99%

Multisource information fusion strategies of mass spectrometry and Fourier transform infrared spectroscopy data for authenticating the age and parts of Vietnamese ginseng

Liu

Zuo

et al. 2021

Journal of Chemometrics

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Aiming at two different classification tasks in the field of quality evaluation of valuable Chinese herbal medicine, the applicability of data fusion strategy based on different complementary analysis techniques was studied. In this study, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and ultra-performance liquid chromatography quadrupole timeof-flight mass spectrometry (UPLC-QTOF/MS) were used to analyze the different parts (including roots, stems, leaves, and fibrils) of 2-to 5-year-old Vietnamese ginseng. The multivariate classification models (orthogonal partial least squares discrimination analysis, OPLS-DA; support vector machine, SVM) were established using low-level and mid-level data fusion methods to identify different parts and age of Vietnamese ginseng. The OPLS-DA model have shown that the fusion data matrix with low-level fusion processing could separate Vietnamese ginseng samples with different growth years to the greatest extent, whereas the single data matrix had partial overlap. But 100% of prediction set classification accuracy could be achieved. The SVM model combined with two parameter optimization algorithms (grid search, GS; genetic algorithm, GA) was used to identify Vietnamese ginseng samples with different ages. The mid-level fusion strategy based on the recursive feature elimination (RFE) features variable extraction method was more suitable for SVM model. In the model established by combining the two parameter optimization methods, the identification effect can reach 83.33%. The results showed that data fusion, as an effective strategy, could distinguish different ages and parts of Vietnamese ginseng.

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Identification of Gentiana rigescens from different geographical origins based on HPLC and FTIR fingerprints

Abstract: Gentiana rigescens is a traditional Chinese medicine with efficacy in liver protection, as a cholagogic, anti-hyperglycemic, and anti-hypertension agent, and in relieving spasms and pain.

Cited by 15 publications

References 35 publications

Gentiana asclepiadea L. from Two High Mountainous Habitats: Inter- and Intrapopulation Variability Based on Species’ Phytochemistry

Gentiana asclepiadea L. from Two High Mountainous Habitats: Inter- and Intrapopulation Variability Based on Species’ Phytochemistry

Chemistry Combining Elemental Profile, Stable Isotopic Ratios, and Chemometrics for Fine Classification of a Chinese Herb Licorice (Glycyrrhiza uralensis Fisch.) from 37 Producing Area

Multisource information fusion strategies of mass spectrometry and Fourier transform infrared spectroscopy data for authenticating the age and parts of Vietnamese ginseng

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