Identification of “
 Haematobacter
 ,” a New Genus of Aerobic Gram-Negative Rods Isolated from Clinical Specimens, and Reclassification of
 Rhodobacter massiliensis
 as “
 Haematobacter massiliensis
 comb. nov.”

Helsel, Leta O.; Hollis, D G; Steigerwalt, Arnold G.; Morey, Roger E.; Jordan, Jean G.; Aye, Tin Tin; Radosevic, Jon; Jannat‐Khah, Deanna; Thiry, Dorothy; Lonsway, David; Patel, Jean B.; Daneshvar, Maryam I.; Levett, Paul N.

doi:10.1128/jcm.01188-06

Cited by 52 publications

(28 citation statements)

References 7 publications

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“…A total of eight 16S rDNA sequences identified were similar to those of bacteria encountered in human clinical specimens, including the species Microbacterium , Klebsiella oxytoca and Haematobacter massiliensis [45,46]. As mosquitoes are mostly known to transmit arboviruses and parasites, it is possible that they also transmit, even on a small scale, opportunistic bacterial pathogens to human and animals.…”

Section: Discussionmentioning

confidence: 99%

Diversity of culturable bacteria including Pantoea in wild mosquito Aedes albopictus

et al. 2013

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BackgroundThe microbiota has been shown to play an important role in the biology of insects. In recent decades, significant efforts have been made to better understand the diversity of symbiotic bacteria associated with mosquitoes and assess their influence on pathogen transmission. Here, we report the bacterial composition found in field-caught Aedes albopictus populations by using culture-dependent methods.ResultsA total of 104 mosquito imagos (56 males and 48 females) were caught from four contrasting biotopes of Madagascar and their bacterial contents were screened by plating whole body homogenates on three different culture media. From 281 bacterial colony types obtained, amplified ribosomal DNA restriction analysis (ARDRA) showed they had 40 distinct ribotypes. Sequencing and BLAST analysis of the 16S rDNA genes responsible for each representative profile made it possible to identify 27 genera distributed in three major phyla. In female mosquitoes, bacterial isolates were mostly Proteobacteria (51.3%) followed by Firmicutes (30.3%) and Actinobacteria (18.4%). Conversely, Actinobacteria was the most abundant phylum in male mosquitoes (48%) followed by Proteobacteria (30.6%) and Firmicutes (20.4%). The relative abundance and composition of isolates also varied between sampling sites, ranging from 3 distinct families in Ankazobe to 8 in Tsimbazaza Park, and Toamasina and Ambohidratrimo. Pantoea was the most common genus in both females and males from all sampling sites, except for Ambohidratrimo. No differences in genome size were found between Pantoea isolates from mosquitoes and reference strains in pulse field gel electrophoresis. However, according to the numbers and sizes of plasmids, mosquito isolates clustered into three different groups with other strains isolated from insects but distinct from isolates from the environment.ConclusionsThe recent upsurge in research into the functional role of the insect microbiota prompts the interest to better explore the role some bacteria detected here may have in the mosquito biology. Future studies of culturable bacteria might decipher whether they have a biological role in the invasiveness of Ae. albopictus. As a possible candidate for paratransgenesis, the predominant genus Pantoea will be characterized to better understand its genetic contents and any possible influence it may have on vector competence of Ae. albopictus.

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Section: Discussionmentioning

confidence: 99%

Diversity of culturable bacteria including Pantoea in wild mosquito Aedes albopictus

et al. 2013

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“…The isolate identified as H. massiliensis showed high MICs towards different classes of antibiotics including aminoglycosides, fluoroquinolones, tetracycline, chloramphenicol, and piperacillin. Tetracycline and chloramphenicol are usually effective against this species isolated from clinical specimens (Helsel et al 2007). In our study, these molecules displayed a poor activity against the strain isolated in Micriamoeba.…”

Section: Discussionmentioning

confidence: 99%

Diversity of free-living amoebae in soils and their associated human opportunistic bacteria

et al. 2017

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Free-living amoebae (FLA) are ubiquitous protozoa found worldwide in the environment. They feed by phagocytosis on various microorganisms. However, some bacteria, i.e., amoebae-resistant bacteria (ARB) or bacterial endocytobionts, can resist phagocytosis and even multiply inside FLA. This study investigated the diversity of culturable FLA in various soils from agricultural and mining sites and their bacterial endocytobionts. FLA were cultured on non-nutrient agar with alive Escherichia coli and identified by PCR and sequencing. Amoebae were lysed and bacterial endocytobionts were cultured on TSA 1/10 and Drigalski medium. Bacterial isolates were identified by PCR and 16S rDNA sequencing and characterized for their antibiotic resistance properties. To measure bacterial virulence, the amoebal model Dictyostelium discoideum was used. The analysis of FLA diversity showed that Tetramitus was the most prevalent genus in agricultural soil from Burkina Faso (73%) and garden soil from Vietnam (42%) while Naegleria and Acanthamoeba were dominant genera in mining soil from Vietnam (55%) and French alpine soil (77%). Some genera were only present in one out of the four soils analyzed. The bacterial endocytobiont included Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria. Human opportunistic pathogens identified as Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Burkholderia cepacia were found associated with amoebae including Micriamoeba, Tetramitus, Willaertia, or Acanthamoeba. Some of these bacteria showed various antibiotic resistance phenotypes and were virulent. Our study confirms that the occurrence of these opportunistic bacteria with FLA in soils may be important for the survival, multiplication, and spread of pathogens in the environment.

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“…Table 1. Physiological characteristics of strain YYQ-30 T and related genera of the family Rhodobacteraceae Taxa: 1, strain YYQ-30 T ; 2, Albimonas (n52; data from Li et al, 2013a;Lim et al, 2008); 3, Rhodovulum (n518; Chakravarthy et al, 2009;Hiraishi & Ueda, 1994;Imhoff et al, 1984;Lakshmi et al, 2011;Nupur et al, 2014;Srinivas et al, 2012Srinivas et al, , 2014; 4, Albidovulum (n52; Albuquerque et al, 2002;Yin et al, 2012); 5, Haematobacter (n52; Greub & Raoult, 2003;Helsel et al, 2007); 6, Tropicimonas (n53; Harwati et al, 2009;Oh et al, 2012;Shin et al, 2012); 7, Paenirhodobacter (n51; Wang et al, 2014); 8, Dinoroseobacter (n51; Biebl et al, 2005); 9, Gemmobacter (n510; Anil Kumar et al, 2007;Chen et al, 2013, Liu et al, 2010Rothe et al, 1987, Tanaka et al, 2004; 10, Roseovarius (n515; Boettcher et al, 2005;Choi et al, 2013;Jung et al, 2012;Kim et al, 2012b;Labrenz et al, 1999;Lai et al, 2011;Li et al, 2013b;Oh et al, 2009;Park & Yoon, 2013;Yoon et al, 2008). +, Positive; 2, negative; V, variable; W, weakly positive; ND, no data.…”

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confidence: 99%

Halovulum dunhuangense gen. nov., sp. nov., isolated from a saline terrestrial spring

Sun

Liu

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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A bacterial strain, T , isolated from a mixed water-sand-sediment sample collected from a terrestrial spring located in Dunhuang, China, was characterized with respect to its morphology, physiology and taxonomy. Cells of the strain were Gram-stain-negative, aerobic, oxidase-and catalase-positive, non-flagellated, oval to rod-shaped (0.5-1.0 mm wide and 1.1-6.6 mm long) and divided by binary fission. Growth was observed in the presence of 0-10.0 % (w/v) NaCl with optimal growth at 0-3.0 %, at pH 6.0-9.0 (optimum pH 7.0-8.5) and at 10-45 8C (optimum 30-37 8C). The isolate could reduce nitrate to nitrite and hydrolyse aesculin and gelatin (weakly), but was unable to degrade Tween 80 or starch. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YYQ-30 T belongs to the family Rhodobacteraceae and forms a distinct lineage with the type strain of Albimonas donghaensis and forms a branch within a cluster constituted by the type strains of species of the genera Albimonas, Rhodovulum, Albidovulum, Haematobacter and Tropicimonas; levels of 16S rRNA gene sequence similarity between strain YYQ-30 T and members of related genera ranged from 94.1 to 89.7 %. Strain YYQ-30 T contained Q-10 as the predominant ubiquinone and summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6c; 70.0 %), C 18 : 0 (9.5 %), summed feature 2 (one or more of C 14 : 0 3-OH, iso-C 16 : 1 I and C 12 : 0 aldehyde; 6.9 %) and 11-methyl C 18 : 1 v7c (6.0 %) as the principal fatty acids. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids, two unidentified aminolipids and five unknown lipids. The pufLM gene was detected. The G+C content of the genomic DNA was 71.7 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained in this study, strain YYQ-30 T is considered to represent a novel species in a new genus within the family Rhodobacteraceae, for which the name Halovulum dunhuangense gen. nov., sp. nov. is proposed. The type strain of Halovulum dunhuangense is YYQ-30During an investigation of bacterial diversity of a terrestrial spring, strain YYQ-30 T was isolated and characterized taxonomically along with several other bacterial isolates. Comparative 16S rRNA gene sequence analysis indicated that strain YYQ-30 T belongs to the family Rhodobacteraceae. However, it could not be assigned to any genus because of its low sequence similarity to any type strain (,94.1 %). The type genus of the family Rhodobacteraceae, Rhodobacter, was proposed by Imhoff et al. (1984) and, at the time of writing, the family comprised 105 genera (http://www.bacterio.net/rhodobacteraceae.html). Consequently, the aim of the present work was to determine the exact taxonomic position of strain T by using a polyphasic approach.A mixed water-sand-sediment sample was collected from a terrestrial spring named Crescent Moon Spring located at the foot of Echoing-Sand Mountain in the desert of Dunhuang County, Gansu Province, China (40.58 N 94.408 E) and used for bacter...

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Identification of “ Haematobacter ,” a New Genus of Aerobic Gram-Negative Rods Isolated from Clinical Specimens, and Reclassification of Rhodobacter massiliensis as “ Haematobacter massiliensis comb. nov.”

Cited by 52 publications

References 7 publications

Diversity of culturable bacteria including Pantoea in wild mosquito Aedes albopictus

Diversity of culturable bacteria including Pantoea in wild mosquito Aedes albopictus

Diversity of free-living amoebae in soils and their associated human opportunistic bacteria

Halovulum dunhuangense gen. nov., sp. nov., isolated from a saline terrestrial spring

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