2005
DOI: 10.1016/j.femsle.2005.01.055
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Identification ofN-acylhomoserine lactones in mucopurulent respiratory secretions from cystic fibrosis patients

Abstract: Pseudomonas aeruginosa and species of the Burkholderia cepacia complex are the primary bacterial pathogens contributing to lung disease in patients with cystic fibrosis. Quorum sensing systems using N-acyl homoserine lactone (AHL) signal molecules are involved in the regulation of a number of virulence factors in these species. Extracts of mucopurulent respiratory secretions from 13 cystic fibrosis patients infected with P. aeruginosa and/or strains of the B. cepacia complex were fractionated using reverse-pha… Show more

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Cited by 93 publications
(92 citation statements)
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“…The most common used methods of extraction of AI molecules are liquid-liquid extraction (LLE) and solid-phase extraction (SPE). In LLE an organic solvent such as dichloromethane, hexane, ethyl acetate, or ethyl esters are used for the extraction, then the solvent is dried leaving only the extracted molecules, which are usually re-dissolved in methanol or in acetonitrile-water (McClean et al, 1997;Chambers et al, 2005;Pearson et al, 1994;Pearson et al, 1995). In SPE, the sample is first extracted with an organic solvent such as methanol or ethyl acetate.…”
Section: E Analytical Techniques To Detect Extracellular Moleculesmentioning
confidence: 99%
“…The most common used methods of extraction of AI molecules are liquid-liquid extraction (LLE) and solid-phase extraction (SPE). In LLE an organic solvent such as dichloromethane, hexane, ethyl acetate, or ethyl esters are used for the extraction, then the solvent is dried leaving only the extracted molecules, which are usually re-dissolved in methanol or in acetonitrile-water (McClean et al, 1997;Chambers et al, 2005;Pearson et al, 1994;Pearson et al, 1995). In SPE, the sample is first extracted with an organic solvent such as methanol or ethyl acetate.…”
Section: E Analytical Techniques To Detect Extracellular Moleculesmentioning
confidence: 99%
“…Until recently, the detection of quorum sensing molecules in clinical specimens has relied on the use of biosensors [42,80,83,[90][91][92][93][94][95]. This technique has previously required collection of a clinical sample, culturing the P. aeruginosa on selective agar, selecting a colony, co-culturing with reporter strains then measuring turbidity and fluorescence.…”
Section: Detection Of Quorum Sensing Molecules In Clinical Samplesmentioning
confidence: 99%
“…The detection range of quorum sensing molecules in clinical samples with biosensors is in the nanomolar range for C4-HSL (2500 -0.0025 nM) and in the picomolar range for 3-oxo-C12-HSL (20 nM-0.002 pM) [93]. In mass spectrometry, sensitivity (to date) is not as good, with clinical detection in the micromolar range (0.25 -1.4 µM) dependent upon the culture conditions [98].…”
Section: Detection Of Quorum Sensing Molecules In Clinical Samplesmentioning
confidence: 99%
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