“…The most common used methods of extraction of AI molecules are liquid-liquid extraction (LLE) and solid-phase extraction (SPE). In LLE an organic solvent such as dichloromethane, hexane, ethyl acetate, or ethyl esters are used for the extraction, then the solvent is dried leaving only the extracted molecules, which are usually re-dissolved in methanol or in acetonitrile-water (McClean et al, 1997;Chambers et al, 2005;Pearson et al, 1994;Pearson et al, 1995). In SPE, the sample is first extracted with an organic solvent such as methanol or ethyl acetate.…”