Identification of Staphylococcus Argenteus in Eastern China based on a Nonribosomal Peptide Synthetase ( NRPS ) Gene

Abstract

The PCR-based method targeting the NRPS gene can simultaneously identify and distinguish S. argenteus and S. aureus. All representative sequences of rpoB generated in this study were deposited in GenBank under accession numbers SJTU F20002, KT767581; SJTU F20269, KT767582; SJTU F20419, KT767583; SJTU F20420, KT767584; SJTU F20124, KT767585; SJTU F21164, KT767586; SJTU F21285, KT767587; SJTU F21224, KT767588; SJTU F21155, KT767589; SJTU F21294, KT767590; SJTU F20030, KT767591; SJTU F20044, KT767592; SJTU F20135, KT767593; SJTU F20123, KT767594; SJTU F21319, KT767595, respectively. All the new sequence types (STs) were submitted to a multilocus sequence typing database and the assigned ST numbers are ST3261 (151-469-20-101-145-150-131), ST3262 (12-3-1-1-4-4-410) and ST3267 (2-471-2-2-6-3-2).

Staphylococcus argenteus from rabbits in Thailand

“…The NRPS gene has been identified in studies of a diverse array of related S. aureus and S. argenteus . This study found that NRPS amplification can differentiate S. argenteus from S. aureus , as also reported previously (Zhang et al., ). The sequences of NRPS amplicons analyzed using the GenBank database also corresponded to the MALDI‐TOF MS results.…”

“…The results showed that three isolates (U19 T10.1, U43 S18.1, and U43 S18.3) have a PCR product of approximately 340 bp, which was correlated with S. argenteus as reported by Zhang et al (2016). In contrast, the five other suspected isolates had a PCR product of nearly 160 bp, which was reported to correlate with S. aureus (Zhang et al, 2016). The sequences of NRPS amplicons were analyzed for similarity using the GenBank database, which showed that bacterial isolate no.…”

“…(). In contrast, the five other suspected isolates had a PCR product of nearly 160 bp, which was reported to correlate with S. aureus (Zhang et al., ). The sequences of NRPS amplicons were analyzed for similarity using the GenBank database, which showed that bacterial isolate no.…”

“…The NRPS gene was amplified for eight suspected S. argenteus isolates, as shown in Figure 1. The results showed that three isolates (U19 T10.1, U43 S18.1, and U43 S18.3) have a PCR product of approximately 340 bp, which was correlated with S. argenteus as reported by Zhang et al (2016). In contrast, the five other suspected isolates had a PCR product of nearly 160 bp, which was reported to correlate with S. aureus (Zhang et al, 2016).…”

“…Moreover, identification by molecular methods, such as 16S rRNA sequencing, cannot differentiate S. argenteus from S. aureus (Tong et al., ). Other molecular techniques, such as matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS), nonribosomal peptide synthetase (NRPS) gene amplification, and multilocus sequence type (MLST) determination, were thus recommended in many publications as tools for the identification of S. argenteus (Chantratita et al., ; Schuster et al., ; Zhang et al., ). Some sequencing types of S. aureus were previously confirmed to be S. argenteus , such as ST2793, ST1223, and ST2250 (Chantratita et al., ; Schuster et al., ; Thaipadungpanit et al., ; Tong et al., ).…”