Identification of immunodominant regions of Brassica juncea glyoxalase I as potential antitumor immunomodulation targets

Deswal, Renu; Singh, Rohini; Lynn, Andrew M.; Frank, Ronald

doi:10.1016/j.peptides.2004.10.028

Cited by 5 publications

(3 citation statements)

References 46 publications

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“…The most common types of filter or chromatography paper used for SPOT synthesis are Whatman 50, Whatman 540 and *Address correspondence to this author at the Kinexus Bioinformatics Corporation, 8755 Ash Street, Suite 1, Vancouver, BC V6P 6T3, Canada; Tel: +604-323-2547 ext.17; Fax: +604-323-2548; E-mail: peptides@kinexus.ca CHR1 [23][24][25][26]. N-methyl-imidazole (NMI) [27,28]. These functional groups are the only groups accessible for the build-up of peptide chains on cellulose.…”

Section: Modification Of the Cellulose Membranementioning

confidence: 99%

“…N-terminal acetylation is carried out like capping by treatment of the protected peptide with acetic anhydride at various concentrations [27,54] and often with DIPEA as a basic additive [69] after final Fmoc deprotection. N-terminal acetylation is carried out like capping by treatment of the protected peptide with acetic anhydride at various concentrations [27,54] and often with DIPEA as a basic additive [69] after final Fmoc deprotection.…”

Section: Selected Peptide Modificationsmentioning

confidence: 99%

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Chemistry of SPOT Synthesis for the Preparation of Peptide Macroarrays on Cellulose Membranes

Winkler¹

2011

MROC

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The SPOT technique is one of the most frequently used methods for synthesis and screening of peptides on arrays. Materials such as polypropylene and glass are used for the preparation of peptide arrays, however the most commonly used material for SPOT membranes is cellulose. This paper focuses on materials and procedures used in the SPOT synthesis on cellulose membranes as a special type of solid phase peptide synthesis. In particular, different strategies for the modification of cellulose are described which make it more suitable for solid-phase peptide synthesis. This review also provides a short overview of the synthesis procedures including some important types of peptide modification.

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Section: Modification Of the Cellulose Membranementioning

confidence: 99%

Section: Selected Peptide Modificationsmentioning

confidence: 99%

Chemistry of SPOT Synthesis for the Preparation of Peptide Macroarrays on Cellulose Membranes

Winkler¹

2011

MROC

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“…Hence, in many cases synthetic peptides or peptidomimetics, which mimic the sequence of the binding site of any given protein, demonstrate the same biological activity as the protein itself, although sometimes with partial loss of activity. Therefore, arrays of synthetic peptides offer a fast and easy possibility to screen for protein interactions [129], binding regions (epitopes) [130,131] and enzyme activities [132,133]. Moreover, they allow the screening for inhibitors [134] and the engineering of antibodies, enzymes or inhibitors [135] with regard to modified binding sites.…”

Section: Peptide Microarraysmentioning

confidence: 99%

Microarray Technology as a Universal Tool for High-Throughput Analysis of Biological Systems

Sobek¹,

Bartscherer²,

Jacob³

et al. 2006

CCHTS

110

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Over the last years microarray technology has become one of the principal platform technologies for the highthroughput analysis of biological systems. Starting with the construction of first DNA microarrays in the 1990s, microarray technology has flourished in the last years and many different new formats have been developed. Peptide and protein microarrays are now applied for the elucidation of interaction partners, modification sites and enzyme substrates. Antibody microarrays are envisaged to be of high importance for the high-throughput determination of protein abundances in translational profiling approaches. First cell microarrays have been constructed to transform microarray technology from an in vitro technology to an in vivo functional analysis tool. All of these approaches share a common prerequisite: the solid support on which they are generated. The demands on this solid support are thereby as manifold as the applications themselves. This review is aimed to display the recent developments in surface chemistry and derivatization, and to summarize the latest developments in the different application areas of microarray technology.

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Cellulose-bound Peptide Arrays: Preparation and Applications

Hilpert

Winkler

Hancock

2007

Biotechnology and Genetic Engineering Reviews

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Identification of immunodominant regions of Brassica juncea glyoxalase I as potential antitumor immunomodulation targets

Cited by 5 publications

References 46 publications

Chemistry of SPOT Synthesis for the Preparation of Peptide Macroarrays on Cellulose Membranes

Chemistry of SPOT Synthesis for the Preparation of Peptide Macroarrays on Cellulose Membranes

Microarray Technology as a Universal Tool for High-Throughput Analysis of Biological Systems

Cellulose-bound Peptide Arrays: Preparation and Applications

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