Identification of immunogenic proteins and evaluation of four recombinant proteins as potential vaccine antigens from Vibrio anguillarum in flounder ( Paralichthys olivaceus )

Xing, Jing; Xu, Hongsen; Wang, Yan; Tang, Xiaoqian; Sheng, Xiuzhen; Zhan, Wenbin

doi:10.1016/j.vaccine.2017.04.071

Cited by 21 publications

(12 citation statements)

References 41 publications

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“…The rVAA protein was expressed and purified from E. coli, before being used for polyclonal antibody production in mice, as previously reported (6,17). Western blot analysis revealed that the resulting antiserum specifically bound the purified rVAA, confirming the specificity of produced polyclonal antibodies (Supplementary Figure 2).…”

Section: Antibodies Cells Bacteria and Animalssupporting

confidence: 62%

“…In our previous study, VAA was identified by western blotting with fish antibodies. It was demonstrated that the recombinant protein emulsified with Freund's complete adjuvant was proven to induce a humoral immune response in injected fish, conferring a RPS of 78.38% for flounder (6). Considering the previously observed immunoprotective effect provided by rVAA, the recombinant DNA plasmid assessed here may seem less attractive as it resulted in a lower RPS of 50.00%.…”

Section: Discussionmentioning

confidence: 89%

“…In a previous study, we successfully identified the immunogenic VAA protein from V. anguillarum. Immunization of fish with recombinant VAA (rVAA) protein has been shown to give rise to a humoral immune response that confers effective immune protection against V. anguillarum challenge (6). The successful design of a DNA vaccine and determination of its efficacy requires that any candidate results in a potent stimulation of the immune system, either via humoral, or cellular immune responses (7,8).…”

Section: Introductionmentioning

confidence: 99%

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A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Xing

Tang

et al. 2019

Front. Immunol.

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Vibrio anguillarum is a pathogenic bacterium that infects flounder resulting in significant losses in the aquaculture industry. The VAA protein previously identified in flounder is associated with a role in immune protection within these fish. In the present study, a recombinant DNA plasmid encoding the VAA gene of V. anguillarum was constructed and its potential as a DNA vaccine, to prevent the infection of V. anguillarum in flounder fish, investigated. We verified the expression of the VAA protein both in vitro in cell lines and in vivo in flounder fish. The protective effects of pcDNA3.1-VAA (pVAA) were analyzed by determination of the percentage of sIgM+, CD4-1+, CD4-2+, CD8β+ lymphocytes, and the production of VAA-specific antibodies in flounder following their immunization with the DNA vaccine. Histopathological changes in immune related tissues, bacterial load, and relative percentage survival rates of flounder post-challenge with V. anguillarum, were all investigated to assess the efficacy of the pVAA DNA vaccine candidate. Fish intramuscularly immunized with pVAA showed a significant increase in CD4-1+, CD4-2+, and CD8β+ T lymphocytes at days 9, 11, and 14 post-vaccination, reaching peak T-cell levels at days 11 or 14 post-immunization. The percentage of sIgM+ lymphocytes reached peak levels at weeks 4–5 post-immunization. Specific anti-V. anguillarum or anti-rVAA antibodies were induced in inoculated fish at days 28–35 post-immunization. The liver of vaccinated flounder exhibited only slight histopathological changes compared with a significant pathology observed in control immunized fish. Additionally, a lower bacterial burden in the liver, spleen, and kidney were observed in pVAA protected fish in response to bacterial challenge, compared with pcDNA3.1 vector control injected fish. Moreover, the pVAA vaccine confers a relative percentage survival of 50.00% following V. anguillarum infection. In summary, this is the first study indicating an initial induction of the T lymphocyte response, followed by B lymphocyte induction of specific antibodies as a result of DNA immunization of flounder. This signifies the important potential of pVAA as a DNA vaccine candidate for the control of V. anguillarum infection.

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Section: Antibodies Cells Bacteria and Animalssupporting

confidence: 62%

Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

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A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Xing

Tang

et al. 2019

Front. Immunol.

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“…However, various factors, such as vaccine type, antigen dose, vaccination schedule (with or without booster), vaccine delivery route and the application of adjuvants can influence the vaccine efficacy [95]. Similarly, recent vaccine studies in Vibrio alginolyticus [47,96], Vibrio anguillarum [10,11,97,98], Vibrio harveyi [48], and Edwardsiella tarda [13] indicated that vaccination of recombinant proteins in combination with adjuvants could confer higher RPS levels than immunization with a DNA vaccine alone. Interestingly, pcHrp1, when co-administrated with pcIL-34, could enhance the protection by generating better RPS (82.14%) compared with rHRP1 emulsified with ISA 763 A VG; hence, this result might explain the superiority of IL-34 as an molecular adjuvant.…”

Section: Discussionmentioning

confidence: 99%

Interleukin 34 Serves as a Novel Molecular Adjuvant against Nocardia Seriolae Infection in Largemouth Bass (Micropterus Salmoides)

2020

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DNA vaccines have been widely employed in controlling viral and bacterial infections in mammals and teleost fish. Co-injection of molecular adjuvants, including chemokines, cytokines, and immune co-stimulatory molecules, is one of the potential strategies used to improve DNA vaccine efficacy. In mammals and teleost fish, interleukin-34 (IL-34) had been described as a multifunctional cytokine and its immunological role had been confirmed; however, the adjuvant capacity of IL-34 remains to be elucidated. In this study, IL-34 was identified in largemouth bass. A recombinant plasmid of IL-34 (pcIL-34) was constructed and co-administered with a DNA vaccine encoding hypoxic response protein 1 (Hrp1; pcHrp1) to evaluate the adjuvant capacity of pcIL-34 against Nocardia seriolae infection. Our results indicated that pcIL-34 co-injected with pcHrp1 not only triggered innate immunity and a specific antibody response, but also enhanced the mRNA expression level of immune-related genes encoding for cytokines, chemokines, and humoral and cell-mediated immunity. Moreover, pcIL-34 enhanced the protection of pcHrp1 against N. seriolae challenge and conferred the relative percent survival of 82.14%. Collectively, IL-34 is a promising adjuvant in a DNA vaccine against nocardiosis in fish.

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“…, ), Vibrio anguillarum (Xing et al . ,b) and V. ichthyoenteri (Tang et al . ) that could provide high immunoprotection as potential vaccine antigens have been reported in flounder model in our laboratory, of which, virulence protein A and heat shock protein 33 are the virulence factors of V. anguillarum (Xing et al .…”

Section: Introductionmentioning

confidence: 99%

Recombinant phosphoglucomutase and CAMP factor as potential subunit vaccine antigens induced high protection against Streptococcus iniae infection in flounder (Paralichthys olivaceus )

et al. 2018

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Identification of immunogenic proteins and evaluation of four recombinant proteins as potential vaccine antigens from Vibrio anguillarum in flounder ( Paralichthys olivaceus )

Cited by 21 publications

References 41 publications

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Interleukin 34 Serves as a Novel Molecular Adjuvant against Nocardia Seriolae Infection in Largemouth Bass (Micropterus Salmoides)

Recombinant phosphoglucomutase and CAMP factor as potential subunit vaccine antigens induced high protection against Streptococcus iniae infection in flounder (Paralichthys olivaceus )

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