Identification of immunogenic proteins associated with protection against haemorrhagic septicaemia after vaccination of calves with a live-attenuated aroA derivative of Pasteurella multocida B:2

Ataei, Saeed; Burchmore, Richard; Hodgson, John; Finucane, Anna; Parton, R.; Coote, J. G.

doi:10.1016/j.rvsc.2009.01.007

Cited by 20 publications

(18 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cattle and buffalo are the usual host of HS (Jesse et al, 2013a;. Pasteurella multocida type B: 2 assumed to be transmitted between the animals by aerosol infection and ingestion of contaminated river water or material with P. multocida type B: 2 especially during the HS outbreak (Shafarin et al, 2009;Ataei et al, 2009;Khaleel et al, 2013). The clinical indication of this disease is often characterised by rapid course, high fever, loud and stertorous breathing, profuse salivation, severe depression and followed by death generally within a day of infection Jesse et al, 2013b).…”

Section: Intoductionmentioning

confidence: 99%

“…The key virulence factors identified in Pasteurella multocida type B: 2 include capsule, lipopolysaccharides, surface adhesions, ironregulated and iron acquisition proteins (Affandi et al, 2012;Kharb et al, 2013). The protein toxin from Pasteurella multocidatype B: 2 experimentally induce all of the major symptoms of a number of economically important diseases in domestic livestock (Jamal et al, 2013;Ataei et al, 2009;Faez et al, 2013). However, Pasteurella multocida is nonetheless an effective immunogen and mutant derivatives which showed potential for vaccine development (Ataei et al, 2009).…”

Section: Intoductionmentioning

confidence: 99%

“…The protein toxin from Pasteurella multocidatype B: 2 experimentally induce all of the major symptoms of a number of economically important diseases in domestic livestock (Jamal et al, 2013;Ataei et al, 2009;Faez et al, 2013). However, Pasteurella multocida is nonetheless an effective immunogen and mutant derivatives which showed potential for vaccine development (Ataei et al, 2009). Despite continuing investigations for several decades, the bacterial components LPS have been reported as virulence factor responsible for immuno-pathological changes (Jesse et al, 2013a;2013c).…”

Section: Intoductionmentioning

confidence: 99%

See 2 more Smart Citations

Alterations in Interleukin-1Î² and Interleukin-6 in Mice Inoculated Through the Oral Routes Using Graded Doses of <i>p. Multocida</i> Type b: 2 and its Lipopolysaccharide

Ali¹,

Adamu²,

Abdullah³

et al. 2015

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

The gram-negative bacterium Pasteurella multocida serotype B: 2 is an important veterinary and opportunistic human pathogen that causes deplorable welfare problems and colossal economic production losses in bovine and other animal species throughout the world. Pasteurella multocida type B: 2 and its LPS are associated with Haemorrhagic Septicaemia (HS) in mice as animal model. Sixty healthy Balb c mice were placed in twelve plastic cages each one containing five mice. The mice were divided into three major groups (A, B and C). Group A is the control group (n = 10) and these were inoculated with 0.4 mL of PBS pH 7.4 orally. The treatment groups (B; n = 25 and C; n = 25) were inoculated with P. multocida type B: 2 and lipopolysaccharide respectively. The mice in group B and C were further divided into five subgroups. The subgroups were designated based on the graded doses as B10 1 , B10 3 , B10 5 , B10 7 and B10 9 for Pasteurella multocida and C10 1 , C10 3 , C10 5 , C10 7 and C10 9 for LPS respectively. The mice were observed for 120 h post-inoculation. The concentration of IL-1β was significantly higher (p<0.001) in the B10 7 of P. multocida type B: 2 and C10 9 of LPS compared to the control group and the other treatment groups. The concentration of IL-6 was significantly higher (p<0.001) in the B10 3 cfu of P. multocida type B: 2 and C10 9 cfu of LPS compared to the control group and the other treatment groups. Interleukin-1β and interleukin-6 evaluated in this study were predominantly associated with HS in varying doses of 10 1 , 10 3 , 10 5 , 10 7 and 10 9 in the whole cell and its LPS respectively. Therefore, the target of this study was to investigate on proinflammatory response in mice infected with graded doses of Pasteurella multocida serotype B: 2 and its LPS via the oral route of inoculation.

show abstract

Section: Intoductionmentioning

confidence: 99%

Section: Intoductionmentioning

confidence: 99%

Section: Intoductionmentioning

confidence: 99%

See 1 more Smart Citation

Alterations in Interleukin-1Î² and Interleukin-6 in Mice Inoculated Through the Oral Routes Using Graded Doses of <i>p. Multocida</i> Type b: 2 and its Lipopolysaccharide

Ali¹,

Adamu²,

Abdullah³

et al. 2015

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…multocida , normally an upper respiratory tract commensal, is responsible for HS outbreaks, which mostly occur in hot humid weather, indicating failure of immunity after vaccination or otherwise. The Asian serotype B:2 is mainly responsible for the disease in domestic ruminants (2). Although many of the molecular determinants for virulence of P. multocida are now identified, the pathogenesis of HS is still not well understood (3).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Pasteurella multocida subsp. multocida B:2 Strain VTCCBAA264 Isolated from Bubalus bubalis in North India

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Outer membrane protein (OMP) profiling and western blot analysis have shown several immunogenic proteins in the outer membrane fractions of P. multocida [3,7,13,15]. Among these, a 39 kDa protein has been reported to be highly immunogenic and cross protective, when challenged with heterologous strains [11,21].…”

Section: Introductionmentioning

confidence: 99%

Molecular heterogeneity ofplpE gene in Indian isolates ofPasteurella multocidaand expression of recombinant PlpE in vaccine strain ofP. multocidaserotype B: 2

Singh

Ranjan

et al. 2010

J Vet Sci

View full text Add to dashboard Cite

Outer membrane proteins of Pasteurella (P.) multocida have been known to be protective immunogens. Pasteurella lipoprotein E (PlpE) has been reported to be an important cross reactive outer membrane protein in P. multocida. The gene encoding the PlpE of P. multocida serotypes A: 3, B: 2 and D: 1 was amplified from the genomic DNA. The amplified products were cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clones revealed a single open reading frame of 1,011 bp, 1,008 bp and 1,017 bp encoding a protein with a calculated molecular mass of 37.829 kDa, 37.389 kDa and 37.965 kDa for serotypes A: 3, B: 2 and D: 1 respectively. The comparison of the plpE sequence in different capsular types revealed a high degree (>90%) of homology. Furthermore, the plpE gene of Haemorhhagic septicaemia causing serotype (B: 2) was expressed in E. coli and recombinant PlpE was strongly immunostained by antiserum against whole cell antigen, indicating that the protein is expressed in vivo.

show abstract

Identification of immunogenic proteins associated with protection against haemorrhagic septicaemia after vaccination of calves with a live-attenuated aroA derivative of Pasteurella multocida B:2

Cited by 20 publications

References 20 publications

Alterations in Interleukin-1Î² and Interleukin-6 in Mice Inoculated Through the Oral Routes Using Graded Doses of <i>p. Multocida</i> Type b: 2 and its Lipopolysaccharide

Alterations in Interleukin-1Î² and Interleukin-6 in Mice Inoculated Through the Oral Routes Using Graded Doses of <i>p. Multocida</i> Type b: 2 and its Lipopolysaccharide

Draft Genome Sequence of Pasteurella multocida subsp. multocida B:2 Strain VTCCBAA264 Isolated from Bubalus bubalis in North India

Molecular heterogeneity ofplpE gene in Indian isolates ofPasteurella multocidaand expression of recombinant PlpE in vaccine strain ofP. multocidaserotype B: 2

Contact Info

Product

Resources

About