Identification of immunoglobulin E (IgE)-binding epitopes and recombinant IgE reactivities of a latex cross-reacting Indian jujube Ziz m 1 allergen

Abstract

SummaryZiz m 1 is a major Indian jujube (Zizyphus mauritiana) allergen involved in latex-fruit syndrome, and cDNA of the allergen has been cloned, sequenced and expressed in yeast by our laboratory previously. In this study, we performed an immunoglobulin E (IgE)-binding epitope analysis of Ziz m 1 using overlapping recombinant fragments. Eight overlapping recombinant fragments were generated from the recombinant Ziz m 1 allergen. The fragments were expressed in Escherichia coli and IgE-binding activities were evaluated by sera of latex-Indian jujube-allergic subjects and normal subjects using immunoblotting. , were synthesized, and these peptides reacted with 70%, 100%, 70% and 70% of 10 allergic sera tested, as revealed by enzymelinked immunosorbent assay. Sensitization to 292 VWNRYYDLKT 301 correlated significantly with the presence of allergic symptoms (P < 0·001). These findings will be useful in designing diagnostic and therapeutic approaches, thereby contributing to the development of specific immunotherapy for subjects with latex-fruit syndrome.

A rare case of anaphylaxis to Indian jujube (Ziziphus Mauritiana Lam)

“…Unlike our patient, all cases reported thus far have occurred in patients who have a concomitant latex allergy [1][2][3][4]. Approximately 30-50% of patients who are allergic to latex have evidence of a coexisting food allergy, which is defined as latex-fruit syndrome [7].…”

“…One patient reacted with worsening of their atopic dermatitis and the other patient developed recurrent angioedema of the lips, tongue and throat [2]. Same authors described a case series of ten latex allergic patients who developed symptoms of upper (allergic rhinitis, oral allergy syndrome) and lower respiratory tract (asthma) after consumption of this fruit [4]. Similar to our case report, a case of systemic anaphylaxis to ingestion of five Indian jujubes in a 28-year-old female has been described.…”

“…It is native to tropical and subtropical regions of Asia that include Southern China, India and Malaysia [1][2][3]. A few case reports have described a latex-fruit syndrome where there is a cross-reaction between the allergenic components of latex and the Indian jujube [1][2][3][4][5]. We present the first case of an anaphylactic reaction to this fruit in a patient without a latex allergy.…”

Facilitated antigen presentation and its inhibition by blocking IgG antibodies depends on IgE repertoire complexity

“…29 Other studies indicate that a relationship between IgE clonality and the severity of allergic reactions in peanuts allergy exists. 37,38 In addition, a link between individual IgE specificities and food-induced allergic reactions [39][40][41] and a connection between IgE affinity and the outcomes of skin prick tests 42 have been found. These observations suggest that a further dissection of the IgE repertoire of the individual patient might help to relate IgE levels to disease severity.…”

Current Overview of Allergens of Plant Pathogenesis Related Protein Families

“…Two stretches of residues from Asn72 - Glu86 and from Val292 - Pro320 are the possible IgE binding epitopes characterized in Ziz m 1 [142]. Recent reports suggest that this chitinase can stimulate multiple cytokines, mainly interleukin-13, from peripheral blood mononuclear cells of latex fruit allergic patients [143].…”