Identification of immunoreactive proteins of Dirofilaria immitis and D. repens recognized by sera from patients with pulmonary and subcutaneous dirofilariosis

González‐Miguel, Javier; Brás-Rosário, Luís; Rota-Nodari, Elena; Morchón, Rodrigo; Simón, F.

doi:10.1016/j.parint.2010.02.010

Cited by 17 publications

(17 citation statements)

References 53 publications

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“…Together with mass spectrometry, proteomics allowed the simultaneous identification of numerous proteins, comprising 39 proteins from D. immitis and 15 from D. repens, many of which were represented by several isoforms (157,158,313). These proteins belong primarily to four functional groups, including metabolic enzymes, enzymes with redox or detoxification potential, and molecules involved in motility and the stress response ( Table 1).…”

Section: Classical and Proteomic Approaches To The Study Of Proteins mentioning

confidence: 99%

“…Five proteins with redox potential and four with roles in stress responses, including various heat shock proteins, were also identified. Many of these molecules have antioxidant and detoxification properties and have been linked to the parasites' capacity to neutralize reactive oxygen species released by macrophages and neutrophils (158). Many of the proteins within these groups have also been found in D. repens albeit in a lower number than in D. immitis (Table 1).…”

Section: Classical and Proteomic Approaches To The Study Of Proteins mentioning

confidence: 99%

“…Many glycolytic enzymes have been found in D. immitis (70). The presence of several isoforms of some of those glycolytic enzymes has been demonstrated for both D. immitis and D. repens (158,313), which could provide parasites with an increased ability to thwart any host interference in the energy generation processes required for parasite survival. In contrast, the aerobic metabolism of carbohydrates predominates in microfilariae (188), and oxygen is required for motility although apparently not for survival (207).…”

Section: Energy Generation and Molting In Dirofilaria Speciesmentioning

confidence: 99%

“…A recent analysis of D. immitis and D. repens proteomes and immunomes showed that D. immitis stimulates more vigorous antibody production against its energy metabolism and detoxification machinery than D. repens. If these specific antibodies can block the activity of parasitic enzymes, these data could account for the limited capacity for survival of infective D. immitis larvae over the infection time in human patients, due to worm metabolism blockage, and, consequently, could account for a lack of worm maturation in this host (158).…”

Section: Human Dirofilariasismentioning

confidence: 99%

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Human and Animal Dirofilariasis: the Emergence of a Zoonotic Mosaic

et al. 2012

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SUMMARY Dirofilariasis represents a zoonotic mosaic, which includes two main filarial species ( Dirofilaria immitis and D. repens ) that have adapted to canine, feline, and human hosts with distinct biological and clinical implications. At the same time, both D. immitis and D. repens are themselves hosts to symbiotic bacteria of the genus Wolbachia , the study of which has resulted in a profound shift in the understanding of filarial biology, the mechanisms of the pathologies that they produce in their hosts, and issues related to dirofilariasis treatment. Moreover, because dirofilariasis is a vector-borne transmitted disease, their distribution and infection rates have undergone significant modifications influenced by global climate change. Despite advances in our knowledge of D. immitis and D. repens and the pathologies that they inflict on different hosts, there are still many unknown aspects of dirofilariasis. This review is focused on human and animal dirofilariasis, including the basic morphology, biology, protein composition, and metabolism of Dirofilaria species; the climate and human behavioral factors that influence distribution dynamics; the disease pathology; the host-parasite relationship; the mechanisms involved in parasite survival; the immune response and pathogenesis; and the clinical management of human and animal infections.

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Section: Classical and Proteomic Approaches To The Study Of Proteins mentioning

confidence: 99%

Section: Classical and Proteomic Approaches To The Study Of Proteins mentioning

confidence: 99%

Section: Energy Generation and Molting In Dirofilaria Speciesmentioning

confidence: 99%

Section: Human Dirofilariasismentioning

confidence: 99%

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Human and Animal Dirofilariasis: the Emergence of a Zoonotic Mosaic

et al. 2012

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“…The 2-DE of AgSB was performed as described by González-Miguel et al (2010b). Briefly, AgSB aliquots were supplemented with ampholites and DTT, incubated and centrifuged to remove all particulate material, and then applied to 7-cm IPG strips (Bio-Rad) with linear pH ranges of 3-10, 5-8 and 7-10, using a Protean IEF Cell (Bio-Rad) for isoelectric focusing (IEF).…”

Section: Two-dimensional Electrophoresis and Western Blotmentioning

confidence: 99%