Identification of Inonotus obliquus and Analysis of Antioxidation and Antitumor Activities of Polysaccharides

Abstract

Inonotus obliquus, a wild wood-decay fungus which grows on Betula trees in cool climates, has a variety of biological activities that the scientific community is paying more and more attention to. However, the research work is moving at a snail's pace. The methods of strain identification and the hypha microstructure have not been reported. We isolated one strain of filamentous molds from fruit body which was collected from birch wood on Changbai Mountain, cultivated mycelia on an inclined plane, and examined its micromorphology based on macroscopic examination. The strain was identified as I. obliquus by sequencing its ITS (internal transcribed spacer) domain. We subsequently investigated some of the mycelium polysaccharides' biological activities. The strain used in this study as the producers of antioxidation and anticancer polysaccharides was LNUF008. After fermentation in a 30-L fermenter, mycelia were obtained. The polysaccharides were extracted by transonic recirculation and ethanol precipitation. In order to identify the antioxidation effect, we designed an assay to test the inhibition of endogenous and Fe(2+)-Cys-induced lipid peroxidation as well as ferrous sulfate/ascorbate (Fe(2+)-VC)-induced mitochondrial swelling. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] method was used to study the antiproliferation activity of the polysaccharides on SMMC7721 hepatoma cells. The results indicate that I. obliquus polysaccharides exhibit high antitumor and antioxidation effects. The submerged culture method of growing I. obliquus will enable large-scale production of the polysaccharides.

Exopolysaccharide produced by Bacillus licheniformis strains isolated from Kimchi

“…Statistical signifi cance was defi ned as p 0.05. To evaluate the function of EPSs from B. licheniformis KS-17 and B. licheniformis KS-20, we examined antioxidant activities and compared them to β-glucan extracted from Inonotus obliquus, a medicinal mushroom which is known as an excellent antioxidant polysaccharide (Seo and Lee, 2010;Song et al, 2008). As shown in Fig.…”

Comparative antioxidative characteristics of polysaccharide-enriched extracts from natural sclerotia and cultured mycelia in submerged fermentation of Inonotus obliquus

“…Antioxidant activity has become one of the focuses of the study on mechanisms of the nutraceutical and therapeutical effects of polysaccharides from herbs, microorganisms, and fungi (He, Zhao, Liu, & Zhao, 2001;Song et al, 2008;Zheng et al, 2009). Little information is available about the antioxidative property of the culture broth (the extracellular extract) and the intracellular extract of I. obliquus in submerged culture, and the comparison between the products from the cultured mycelia or the natural sclerotia.…”

Enhancement of exo-polysaccharide production and antioxidant activity in submerged cultures of Inonotus obliquus by lignocellulose decomposition

“…The medicinal mushroom Inonotus obliquus (I. obliquus) belongs to the family Hymenochaetaceae, Basidiomycetes and has long been a folk remedy in Russia and the northern latitudes [1][2][3]. Many triterpenoids, steroids, and phenolic compounds from I. obliquus have various biological activities [1,[4][5][6].…”