Identification of Insecticidal Constituents of the Essential Oil of Curcuma wenyujin Rhizomes Active against Liposcelis bostrychophila Badonnel

Abstract

The aim of this research was to determine the chemical composition and insecticidal activity of the essential oil of Curcuma wenyujin Y.H. Chen et C. Ling rhizomes against the booklouse Liposcelis bostrychophila Badonnel and to isolate any insecticidal constituents from the essential oil. The essential oil of C. wenyujin rhizomes was obtained by hydrodistillation and analyzed by GC-MS. A total of 43 components of the essential oil were identified and the principal compounds in the essential oil were 1,8-cineole (15.26%), camphor (10.12%), germacrone (6.86%), β-elemene (6.33%), curzerene (6.70%), and β-elemenone (5.23%). followed by curzerenone (4.52%), curdione (4.45%) and linalool (4.43%). Based on bioactivity-guided fractionation, the two main active constituents were isolated from the essential oil and identified as 1,8-cineole and camphor. The essential oil of C. wenyujin rhizomes exhibited contact toxicity against L. bostrychophila with an LD50 value of 208.85 µg/cm2. Camphor (LD50 = 207.26 µg/cm2) exhibited stronger contact toxicity than 1,8-cineole (LD50 = 1048.75 µg/cm2) against booklouse. The essential oil of C. wenyujin (LC50 = 2.76 mg/L air) also possessed fumigant toxicity against L. bostrychophila, while the two constituents, camphor and 1,8-cineole had LC50 values of 1.03 mg/L air and 1.13 mg/L air, respectively. The results indicate that the essential oil of C. wenyujin rhizomes and its constituent compounds have potential for development as natural insecticides or fumigants for control of insects in stored grains.

Repellent and Insecticidal Effects of the Essential Oil of <I>Kaempferia galanga</I> Rhizomes to <I>Liposcelis bostrychophila</I> (Psocoptera: Liposcelidae)

“…Contact toxicity of the essential oil against the booklice was measured as described by Liu et al (2012). RangeÞnding studies were run to determine the appropriate testing concentrations of the essential oil of K. galanga and pure compounds.…”

Chemical composition and insecticidal properties of the essential oil of <i>Bidens frondosa</i> L (Asteraceae) against booklice (<i>Liposcelis bostrychophila</i>)

“…However, the essential oil of B. frondosa demonstrated stronger contact toxicity against L. bostrychophila adults than several essential oils using the same bioassay, e.g. Artemisia rupestris [11], A. frigida [12], Curcuma wenyujin [13], Foeniculum vulgare [14], and Valeriana jatamansi [15], however, the essential oil of B. frondosa shows less contact toxicity than the essential oils of Illicium henryi [16] and Kaempferia galangal [17]. Among the four isolated constituents, caryophyllene oxide, and borneol displayed stronger contact toxicity (no overlap in 95 % fiducial limit) than the essential oil against the booklice (L. bostrychophila).…”

“…When compared with dichlorvos (LC 50 = 1.35 μg/L), the essential oil of B. frondosa and 4-terpineol showed only 375 times and 248 times less toxicity against L. bostrychophila, respectively. However, the essential oil of B. frondosa demonstrated a stronger fumigant toxicity against booklice than several essential oils using the same bioassay, e.g., Artemisia rupestris (LC 50 = 6.67 mg/L air) [11], A. frigida (LC 50 =1.25 mg/L) [12], C. wenyujin (LC 50 = 2.76 mg/L) [13], V. jatamansi (LC 50 = 6.0 mg/L) [15], and K. galangal (LC 50 = 1.5 mg/L air) [17], but the oil of B. frondosa demonstrated less toxicity than the essential oils of Allium chinense (LC 50 = 186.5 g/L air) [18] and F. vulgare fruits (LC 50 = 34.07 μg/L) [14]. However, the currently commercial fumigants (e.g.…”

Chemical Composition and Insecticidal Activity of Essential Oil of <i>Artemisia frigida</i> Willd (Compositae) against Two Grain Storage Insects

“…The differences in oil composition was slight as 2 to 3 components However, compared with the fumigant activity of the other essential oils reported in the literature and which were tested using a similar bioassay, the essential oil obtained in the present study exhibited the same or stronger fumigant toxicity against maize weevils, e.g., the essential oils of A. vestita [11], A. eriopoda [13], A. lavandulaefolia and A. sieversiana [15], A. capillaris and A. mongolica [23], A. giraldii and A. subdigitata [12], and A. igniaria [24]. The essential oil of A. frigida aerial parts also possessed the same level of fumigant toxicity to the booklice as that of essential oils of Foeniculum vulgare [16], Illicium pachyphyllum fruits [25], and Curcuma wenyujin rhizomes [26].…”