Identification of interacting proteins of the TaFVE protein involved in spike development in bread wheat

Abstract

WD-40 repeat-containing protein MSI4 (FVE)/MSI4 plays important roles in determining flowering time in Arabidopsis. However, its function is unexplored in wheat. In the present study, coimmunoprecipitation and nanoscale liquid chromatography coupled to MS/MS were used to identify FVE in wheat (TaFVE)-interacting or associated proteins. Altogether 89 differentially expressed proteins showed the same downregulated expression trends as TaFVE in wheat line 5660M. Among them, 62 proteins were further predicted to be involved in the interaction network of TaFVE and 11 proteins have been shown to be potential TaFVE interactors based on curated databases and experimentally determined in other species by the STRING. Both yeast two-hybrid assay and bimolecular fluorescence complementation assay showed that histone deacetylase 6 and histone deacetylase 15 directly interacted with TaFVE. Multiple chromatin-remodelling proteins and polycomb group proteins were also identified and predicted to interact with TaFVE. These results showed that TaFVE directly interacted with multiple proteins to form multiple complexes to regulate spike developmental process, e.g. histone deacetylate, chromatin-remodelling and polycomb repressive complex 2 complexes. In addition, multiple flower development regulation factors (e.g. flowering locus K homology domain, flowering time control protein FPA, FY, flowering time control protein FCA, APETALA 1) involved in floral transition were also identified in the present study. Taken together, these results further elucidate the regulatory functions of TaFVE and help reveal the genetic mechanisms underlying wheat spike differentiation.

Repressor for hire! The vital roles of TOPLESS‐mediated transcriptional repression in plants

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Identification of the WUSCHEL-Related Homeobox (WOX) Gene Family, and Interaction and Functional Analysis of TaWOX9 and TaWUS in Wheat

“…The FVE protein plays important roles in determining flowering time in Arabidopsis [29]. Previous studies have shown that TaFVE directly interacts with multiple proteins to form multiple complexes that regulate the spike developmental process [30]. Those proteins included histone deacetylase, multiple chromatin-remodeling proteins, polycomb-group proteins, multiple flower development regulation factors, and flowering time control proteins.…”

Transcriptomic Insights into Phenological Development and Cold Tolerance of Wheat Grown in the Field

“…Genes related to flower development and meristem identity, SN is almost as cold tolerant as NO under short days with a delayed plant development, but is 405 less cold tolerant than NO under long days (Limin and Fowler, 2002 (VRT2), which inhibits floral meristem identity, are up-regulated by cold in barley (Trevaskis et 411 al., 2007) and wheat (Kane et al, 2005). Three MADS-box transcription factors and one (Jeon and Kim, 2011;Zheng et al, 2017). In addition, the AGAMOUS-…”