Identification of Interspecific Potato Hybrids with Combined Resistance to Late Blight (Phytophthora Infestans) and Nematode (Globodera Rostochiensis)

Abstract

Late blight (agent Phytophthora infestans) and potato cyst nematode (PCN) caused by Globodera rostochiensis are economically important pathogens, which may significantly reduce potato yields. In this study interspecific potato hybrids were used as a source of resistance for combined resistance to economically important potato pathogens: late blight and cyst nematode. The aim of our study was to identify hybrid progenies with combined resistance to both pathogens and to verify the applicability of several molecular markers associated with resistance to G. rostochiensis pathotype Ro1 to identify resistant plants. Ninety-two clones of eleven original interspecific potato hybrids obtained in crosses with the cultivated S. tuberosum group tuberosum, S. tuberosum group Andigena, S. tuberosum group Phureja and wild S. guerreroense, S. microdontum, S. kurtzianum, S. neoantipoviczii and S. tarijense potato species were screened in bioassays and by molecular markers. PCN resistant or moderately resistant clones were found among the progenies of nine hybrids. Results were highly correlated with resistance status detected by molecular markers linked to the H1 (marker 57R) and Gro1-4 (marker Gro1) genes. Marker CP113 (linked to the H1 gene) was not polymorphic and failed to detect resistance status. Combination of foliar late blight resistance and resistance to PCN was identified in hybrids obtained in crosses with plants of species S. microdontum, S. tarijense and S. phureja and in the hybrid between S. guerreroense and Black’s P. infestans race differential carrying gene R-5.

Studies of potato resistance to <i>Globodera rostochiensis</i> revealed novel alleles for 57R marker

“…These results indicate that both markers are useful for resistance detection in the given set of samples. A strong allelic association between 57R and H1 gene compared to other markers for this gene was previously demonstrated by several studies (Khiutti et al 2020, Milczarek et al 2014, Park et al 2018, Schultz et al 2012, Zoteyeva et al 2020. In addition, the reliability of marker TG689 was reported to be pedigree-specific (Schultz et al 2012).…”

“…The initial aim of our research was to decrease the amount of nematode resistance phenotypic tests, to reduce the time needed to get the results and to enable all-the-yearround testing. Genetic analysis has been reported to be an efficient tool for screening for PCN resistance in a number of studies (Asano et al 2021, Biryukova et al 2008, Milczarek et al 2011, 2014, Schultz et al 2012, Zoteyeva et al 2020. PCR-based methods are less time-consuming than phenotypic tests, require less plant material, are easily repeatable and also more informative with allelic dosage (Meiyalaghan et al 2018).…”

Leaf and tuber resistance to <I>Phytophthora infestans</I> and relationship between these traits in wild potato species

“…Список идентифицированных аллелей постоянно пополняется (Jones et al, 2009;Verzaux et al, 2011Verzaux et al, , 2012Sliwka et al, 2012;Tiwari et al, 2014). Весомым аргументом в пользу включения диких видов в селекционные программы является высокая устойчивость к фитофторозу, часто обусловленная наличием в одном генотипе нескольких генов Rpi (Zoteyeva et al, 2017), а также устойчивостью к другим вредным организмам (Chalaya et al, 2012;Zoteyeva et al, 2012Zoteyeva et al, , 2020Radchenko, 2017). Выявленный широкий внутривидовой и внутрипопуляционный полиморфизм по устойчивости к фитофторозу у большинства видов (Zoteyeva, 2019) приводит к необходимости выделения индивидуальных устойчивых растений для привлечения их в гибридизацию.…”