Identification of intest\inal bacteria from Japanese flounder (Paralichthys olivaceus) and their ability to digest chitin

Sugita, Haruo; Itō, Yoko

doi:10.1111/j.1472-765x.2006.01943.x

Cited by 72 publications

(49 citation statements)

References 38 publications

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“…Vibrio has been reported as a typical genus in the intestine of the farmed P. olivaceus (Tanasomwang & Muraga, 1988;Sugita & Ito, 2006), P. dentatus (Eddy & Jones, 2002), S. senegalensis (Tapia-Paniagua et al, 2010), H. hippoglossus (Verner-Jeffreys et al, 2003) and S. maximus (Montes et al, 2003). Intriguingly, in our study, Vibrio was isolated from AF but not from WF.…”

Section: Discussionmentioning

confidence: 48%

“…Despite its negative connotation, Vibrio spp. has a wide enzymatic activity (i.e., amylase, protease, lipase, chitinase) (MacDonald et al, 1986;Sugita & Ito, 2006); and further studies are needed to establish its positive or negative role in AF. Psychrobacter and Acinetobacter have been isolated from marine fish (MacDonald et al, 1986;Tanasomwang & Muroga, 1988;Eddy & Jones, 2002;Sugita et al, 2002;Ringø et al, 2006;Sun et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of the intestinal microbiota of wild caught and farmed fine flounder (Paralichthys adspersus)

Salas‐Leiva

Opazo

Remond

et al. 2017

lajar

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ABSTRACT.A comparative analysis of the cultivable intestinal microbiota of farmed (AF) and wild-caught (WF) Paralichthys adspersus was performed. The 16S rRNA gene was used for taxa identification, and the ITS region for strain differentiation. We detected the presence of Vibrio, Bacillus, Photobacterium, Staphylococcus and Carnobacterium in AF, and Exiguobacterium, Klebsiella, Arthrobacter, Raoultella, Kluyvera, Myroides, Streptococcus, Vagococcus, Staphylococcus, Acinetobacter, Psychrobacter, Lactobacillus, Weissella and Lactococcus in WF. The microbial community was more diverse in WF than in AF. Some bacterial groups were only found in wild-caught fish and may be studied as potential beneficial agents for improving production traits in farmed fish. As the first study of microbiota of P. adspersus, it provides significant information that can potentially help improve farming practices by using strains as species-specific probiotics.

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Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Characterization of the intestinal microbiota of wild caught and farmed fine flounder (Paralichthys adspersus)

Salas‐Leiva

Opazo

Remond

et al. 2017

lajar

View full text Add to dashboard Cite

show abstract

“…Moreover, losing the ability to utilize cellobiose did not result in symbiotic attenuation (Table 3). V. fischeri has been isolated from the water column, even in areas where symbiotic hosts are not found (27,45), and it has also been isolated from the bacterial consortium in the guts of herbivorous marine fishes, where it can survive and persist (42,59). These observations suggest a niche where cellobioase activity may be important for V. fischeri.…”

Section: Discussionmentioning

confidence: 82%

Identification of a Cellobiose Utilization Gene Cluster with Cryptic β-Galactosidase Activity in Vibrio fischeri

Adin

Visick

Stabb

2008

Appl Environ Microbiol

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Cellobiose utilization is a variable trait that is often used to differentiate members of the family Vibrionaceae. We investigated how Vibrio fischeri ES114 utilizes cellobiose and found a cluster of genes required for growth on this ␤-1,4-linked glucose disaccharide. This cluster includes genes annotated as a phosphotransferase system II (celA, celB, and celC), a glucokinase (celK), and a glucosidase (celG). Directly downstream of celCBGKA is celI, which encodes a LacI family regulator that represses cel transcription in the absence of cellobiose. When the celCBGKAI gene cluster was transferred to cellobiose-negative strains of Vibrio and Photobacterium, the cluster conferred the ability to utilize cellobiose. Genomic analyses of naturally cellobiosepositive Vibrio species revealed that V. salmonicida has a homolog of the celCBGKAI cluster, but V. vulnificus does not. Moreover, bioinformatic analyses revealed that CelG and CelK share the greatest homology with glucosidases and glucokinases in the phylum Firmicutes. These observations suggest that distinct genes for cellobiose utilization have been acquired by different lineages within the family Vibrionaceae. In addition, the loss of the celI regulator, but not the structural genes, attenuated the ability of V. fischeri to compete for colonization of its natural host, Euprymna scolopes, suggesting that repression of the cel gene cluster is important in this symbiosis. Finally, we show that the V. fischeri cellobioase (CelG) preferentially cleaves ␤-D-glucose linkages but also cleaves ␤-D-galactose-linked substrates such as 5-bromo-4-chloro-3-indolyl-␤-D-galactoside (X-gal), a finding that has important implications for the use of lacZ as a marker or reporter gene in V. fischeri.

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“…For this reason, transient microorganisms probably have more constant and important interaction with fish gastrointestinal ecosystems compared to terrestrial animals. In general, genus Acinetobacter, Vibrio and Pseudomonas were the most common bacteria in the gut of marine fishes [5,6,7], and Vibrio was considered as one of the predominant bacteria [7,8,9,10]. The experiment measured total bacterial numbers in the gastro intestinal region of three estuarine fishes.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Gut Micro Biota from Some Estuarine Fishes

Sivasubramanian¹,

Ravichandran²,

Kavitha³

2012

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Gut flora has a continuous and dynamic effect on the host's gut and systemic immune systems. The gut microbiota of fish has been studied extensively using various techniques; little information is available on the correlation between gut microbiota and host activity. In the present investigation three species of estuarine fishes were taken with regard to their microbial population in the gut region and enzymatic analysis of those gut isolates. The total heterotrophic bacterial load ranged from 0.8x10 6 to 4.5x10 6 CFU/g of gut sample and it was found to be the maximum of 4.5x10 6 CFU/g in Oreochromis mossambicus gut followed by 3.1x10 6 CFU/g in Oreochromis leucostictus gut and minimum of 0.8x10 6 CFU/g in Etroplus suratenis. The isolates were identified by various biochemical tests. Pseudomonas, Vibrio and Bacilli are the common bacteria found in all three fishes. The bacterial isolates were subjected to quantitative estimation of enzymes viz., amylase, lipase and protease. Varied kinds of results were obtained in different fish species. However it was observed that 20% of the isolates produced only amylase. A collection of 10 bacterial isolates from three fishes were screened for antagonistic activity against human and fish bacterial pathogens. Upon testing all these strains, it became apparent that strains isolated from the fish O.mossambicus, exhibited antimicrobial activity against Klebsiella pneumoniae (17mm) and Vibrio cholerae (12mm). Thus the present preliminary study showed the variation in the gut micro-flora of different fish species and the microbes from marine environment may be useful in development of probiotics, drugs and also in the industrial enzyme production.

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Identification of intest\inal bacteria from Japanese flounder (Paralichthys olivaceus) and their ability to digest chitin

Abstract: The present study shows that Japanese flounder harbours bacteria of the V. scophthalmi-V. ichthyoenteri group, and these results are similar to what has been found for turbot (Scophthalmus maximus).

Cited by 72 publications

References 38 publications

Characterization of the intestinal microbiota of wild caught and farmed fine flounder (Paralichthys adspersus)

Characterization of the intestinal microbiota of wild caught and farmed fine flounder (Paralichthys adspersus)

Identification of a Cellobiose Utilization Gene Cluster with Cryptic β-Galactosidase Activity in Vibrio fischeri

Isolation and Characterization of Gut Micro Biota from Some Estuarine Fishes

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